Molecular basis of the complex formation between the two calcium-binding proteins S100A8 (MRP8) and S100A9 (MRP14) [0.03%]
S100A8(MRP8)与S100A9(MRP14)两个钙结合蛋白形成复合物的分子基础研究
Nadja Leukert,Clemens Sorg,Johannes Roth
Nadja Leukert
S100 proteins form characteristic homo- and/or heterodimers that play a role in calcium-mediated signaling. We characterized the formation of the human S100A8/S100A9 heterodimer using the yeast two-hybrid system. Employing site-directed mut...
Evolution of vitamin B2 biosynthesis: riboflavin synthase of Arabidopsis thaliana and its inhibition by riboflavin [0.03%]
维生素B2生物合成的进化：阿拉伯芥核糖FLV合酶及核黄素对其抑制作用
Markus Fischer,Ilka Haase,Richard Feicht et al.
Markus Fischer et al.
A synthetic gene specifying the catalytic domain of the Arabidopsis thaliana riboflavin synthase was expressed with high efficiency in a recombinant Escherichia coli strain. The recombinant pseudomature protein was shown to convert 6,7-dime...
Saccharomyces cerevisiae translational activator Cbs1p is associated with translationally active mitochondrial ribosomes [0.03%]
酿酒酵母转译激活因子Cbs1p与转译活跃的线粒体核糖体相关
Udo Krause-Buchholz,Karina Schöbel,Susann Lauffer et al.
Udo Krause-Buchholz et al.
In the yeast Saccharomyces cerevisiae, mitochondrial translation of most, if not all, mitochondrially encoded genes is regulated by an individual set of gene-specific activators. Translation of the COB mRNA encoding cytochrome b requires th...
Labelling of four distinct trophozoite falcipains of Plasmodium falciparum by a cystatin-derived probe [0.03%]
一种来源于半胱氨酸蛋白酶抑制剂的荧光探针同时标记恶性疟原虫四种不同的裂殖体丝状体期半胱氨酸蛋白酶
Isabelle Florent,Fabien Lecaille,Jean-Jacques Montagne et al.
Isabelle Florent et al.
Trophozoite cysteine protease (TCP) activity, isolated from Plasmodium falciparum soluble 100,000 g extracts, displayed native falcipain-1 kinetic parameters towards peptidyl substrates. The labelling of either isolated TCP or soluble 100,0...
Proteolytic susceptibility of the serine protease inhibitor trappin-2 (pre-elafin): evidence for tryptase-mediated generation of elafin [0.03%]
弹性蛋白酶抑制剂trappin-2(前elafin)的蛋白水解敏感性：证据表明组胺酶介导生成elafin
Nicolas Guyot,Marie-Louise Zani,Patrick Berger et al.
Nicolas Guyot et al.
A number of serine, cysteine, metallo- and acid proteases were evaluated for their ability to proteolytically cleave the serine protease inhibitor trappin-2, also known as pre-elafin, and to release elafin from its precursor. None of the me...
cDNA cloning and heterologous expression of a wheat proteinase inhibitor of subtilisin and chymotrypsin (WSCI) that interferes with digestive enzymes of insect pests [0.03%]
一种能干扰昆虫害虫消化酶的小麦蛋白酶抑制剂(WSCI)cDNA的克隆和异源表达
Simone Di Gennaro,Anna G Ficca,Daniela Panichi et al.
Simone Di Gennaro et al.
A cDNA encoding the proteinase inhibitor WSCI (wheat subtilisin/chymotrypsin inhibitor) was isolated by RT-PCR. Degenerate oligonucleotide primers were designed based on the amino acid sequence of WSCI and on the nucleotide sequence of the ...
Visualisation of transforming growth factor-beta 1, tissue kallikrein, and kinin and transforming growth factor-beta receptors on human clear-cell renal carcinoma cells [0.03%]
人透明细胞肾癌细胞中转化生长因子-β1、组织激肽释放酶和缓激肽以及转化生长因子-β受体的可视化研究
Rumesha Moodley,Celia Snyman,Bharti Odhav et al.
Rumesha Moodley et al.
Transforming growth factor-beta1 (TGF-beta1) has a biphasic effect on the growth of renal epithelial cells. In transformed cells, TGF-beta1 appears to accelerate the proliferation of malignant cells. The diverse cellular functions of TGF-be...
Comparative Study
Biological chemistry. 2005 Apr;386(4):375-82. DOI:10.1515/BC.2005.045 2005
The snake venom metalloproteases berythractivase and jararhagin activate endothelial cells [0.03%]
伯厄特拉激活酶和贾阿拉毒素可以活化内皮细胞
Mirta Schattner,Márcio Fritzen,Janaína de Souza Ventura et al.
Mirta Schattner et al.
PIII snake venom metalloproteases (SVMPs) are metalloproteases structurally related to ADAMs (a disintegrin and metalloprotease human family of proteins). Berythractivase and jararhagin are PIII SVMPs with 69% homology that have different h...
Comparative Study
Biological chemistry. 2005 Apr;386(4):369-74. DOI:10.1515/BC.2005.044 2005
P. falciparum pro-histoaspartic protease (proHAP) protein peptides bind specifically to erythrocytes and inhibit the invasion process in vitro [0.03%]
疟原虫前-组胺天冬氨酸蛋白酶（proHAP）多肽特异性结合红细胞并抑制体外入侵过程
John Valbuena,Ricardo Vera,Alvaro Puentes et al.
John Valbuena et al.
Plasmodium falciparum histoaspartic protease (HAP) is an active enzyme involved in haemoglobin degradation. HAP is expressed as an inactive 51-kDa zymogen and is cleaved into an active 37-kDa enzyme. It has been proposed that this kind of p...
Interpretation of the reactivity of peroxidase compound II with phenols and anilines using the Marcus equation [0.03%]
Marcus方程指导的过氧化物酶复合体II与酚和芳胺反应活性的理论研究
Lorena G Fenoll,Francisco García-Molina,María A Gilabert et al.
Lorena G Fenoll et al.
The catalytic cycle of heme peroxidases involves three processes: the formation of compound I, its conversion to compound II and regeneration of the native enzyme. Each of the processes consists of a reversible binding stage followed by an ...