The NC1 dimer of human placental basement membrane collagen IV: does a covalent crosslink exist? [0.03%]
人胎盘基底膜IV型胶原的NC1二聚体：是否存在共价交联？
Manuel E Than,Gleb P Bourenkov,Stefan Henrich et al.
Manuel E Than et al.
Triple-helical collagen IV protomers associate through their N- and C-termini, forming a three-dimensional network that provides basement membranes with mechanical strength. Within this network, the C-terminal non-collagenous (NC1) domains ...
Efficient production of native actin upon translation in a bacterial lysate supplemented with the eukaryotic chaperonin TRiC [0.03%]
一种高效原核细胞裂解体系利用真核分子伴侣TRiC辅助表达纯化全长人源G-actin蛋白质的新方法
Markus J Stemp,Suranjana Guha,F Ulrich Hartl et al.
Markus J Stemp et al.
Recombinant expression of actin in bacteria results in non-native species that aggregate into inclusion bodies. Actin is a folding substrate of TRiC, the chaperonin of the eukaryotic cytosol. By employing bacterial in vitro translation lysa...
Anton Eberharter,Roger Ferreira,Peter Becker
Anton Eberharter
The flexibility of chromatin that enables translation of environmental cues into changes in genome utilisation, relies on a battery of enzymes able to modulate chromatin structure in a highly targeted and regulated manner. The most dynamic ...
Novel insights into the mechanism of chaperone-assisted protein disaggregation [0.03%]
伴侣介导的蛋白质解聚的新见解
Jimena Weibezahn,Christian Schlieker,Peter Tessarz et al.
Jimena Weibezahn et al.
Cell survival under severe thermal stress requires the activity of a bi-chaperone system, consisting of the ring-forming AAA+ chaperone ClpB (Hsp104) and the DnaK (Hsp70) chaperone system, which acts to solubilize and reactivate aggregated ...
Marion Schmidt,John Hanna,Suzanne Elsasser et al.
Marion Schmidt et al.
The proteasome is a compartmentalized, ATP-dependent protease composed of more than 30 subunits that recognizes and degrades polyubiquitinated substrates. Despite its physiological importance, many aspects of the proteasome's structural org...
Splicing of a rare class of introns by the U12-dependent spliceosome [0.03%]
由U12依赖性剪接体介导的一类稀有内含子的拼接
Cindy L Will,Reinhard Lührmann
Cindy L Will
Pre-mRNA splicing is catalyzed by two unique spliceosomes, designated U2- or U12-dependent. In contrast to the well-characterized U2-dependent spliceosome, much remains to be learned about the less abundant U12-type spliceosome. This review...
Highlight: molecular machines [0.03%]
聚焦：分子机器
Walter Neupert
Walter Neupert
Activation processing of cathepsin H impairs recognition by its propeptide [0.03%]
猫hepsin H的活化加工影响其前肽的识别作用
Martin Horn,Lucie Dolecková-Maresová,Lubomír Rulísek et al.
Martin Horn et al.
Free propeptides are known to function as inhibitors of the parental mature cysteine cathepsins. This general rule, however, does not apply to the aminopeptidase cathepsin H. Screening of propeptide fragments for their inhibitory potency re...
Inhibition of cathepsin B reduces beta-amyloid production in regulated secretory vesicles of neuronal chromaffin cells: evidence for cathepsin B as a candidate beta-secretase of Alzheimer's disease [0.03%]
猫肉豆蔻B抑制可减少神经肾上腺素能细胞调节分泌囊泡中β-淀粉样蛋白的产生：猫肉豆蔻B是阿尔茨海默氏病β-分泌酶候选者的证据
Vivian Hook,Thomas Toneff,Matthew Bogyo et al.
Vivian Hook et al.
The regulated secretory pathway of neurons is the major source of extracellular A beta that accumulates in Alzheimer's disease (AD). Extracellular A beta secreted from that pathway is generated by beta-secretase processing of amyloid precur...
Focal adhesion kinase is redistributed to focal complexes and mediates cell spreading in macrophages in response to M-CSF [0.03%]
焦点黏附蛋白激酶在巨噬细胞中重新分布至焦点复合物并介导M-CSF响应下的细胞铺展现象
Elisabetta Rovida,Benedetta Lugli,Valentina Barbetti et al.
Elisabetta Rovida et al.
The macrophage colony-stimulating factor (M-CSF, CSF-1) regulates survival, proliferation and differentiation of mononuclear phagocytes, as well as macrophage motility and morphology. The latter features are usually regulated by ECM-mediate...