Efficient production of native actin upon translation in a bacterial lysate supplemented with the eukaryotic chaperonin TRiC
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Recombinant expression of actin in bacteria results in non-native species that aggregate into inclusion bodies. Actin is a folding substrate of TRiC, the chaperonin of the eukaryotic cytosol. By employing bacterial in vitro translation lysates supplemented wit... ...
