Inhibition of human kallikreins 5 and 7 by the serine protease inhibitor lympho-epithelial Kazal-type inhibitor (LEKTI) [0.03%]
Norman M Schechter,Eun-Jung Choi,Zhe-Mei Wang et al.
Norman M Schechter et al.
LEKTI is a 120-kDa protein that plays an important role in skin development, as mutations affecting LEKTI synthesis underlie Netherton syndrome, an inherited skin disorder producing severe scaling. Its primary sequence indicates that the pr...
B-Raf and C-Raf signaling investigated in a simplified model of the mitogenic kinase cascade [0.03%]
Armin Robubi,Tobias Mueller,Jochen Fueller et al.
Armin Robubi et al.
Signaling pathways based on the reversible phosphorylation of proteins control most aspects of cellular life in higher organisms. Extracellular stimuli can induce growth, differentiation, survival and the stress response through a number of...
Ralf P Meyer,Raija L P Lindberg,Francine Hoffmann et al.
Ralf P Meyer et al.
Previous work has demonstrated that the function of extrahepatic cytochrome P450 CYP1A1 is dependent on the availability of heme. CYP1A1 is involved in the activation of polyaromatic hydrocarbons. In the present study we used a transgenic m...
The polysaccharide scaffold of PrP 27-30 is a common compound of natural prions and consists of alpha-linked polyglucose [0.03%]
Christian Dumpitak,Michael Beekes,Nicole Weinmann et al.
Christian Dumpitak et al.
An inert polysaccharide scaffold identified as a 5-15% component of prion rods (PrP 27-30) is unambiguously distinguishable from the N-glycosyl groups and the GPI anchor of PrP, and consists predominantly of 1,4-linked glucose with some bra...
Lysophosphatidic acid affinity chromatography reveals pyruvate kinase as a specific LPA-binding protein [0.03%]
Sophie Desmaret,Lian Qian,Berlinda Vanloo et al.
Sophie Desmaret et al.
Lysophosphatidic acid is a pleiotropic lipid signaling molecule that evokes a broad array of cellular responses including proliferation, tumor cell invasion, neurite retraction, cytoskeletal rearrangements and smooth muscle contraction. Gen...
Christine Nickel,Madia Trujillo,Stefan Rahlfs et al.
Christine Nickel et al.
Thioredoxin peroxidase 1 (TPx1) of the malarial parasite Plasmodium falciparum is a 2-Cys peroxiredoxin involved in the detoxification of reactive oxygen species and - as shown here - of reactive nitrogen species. As novel electron acceptor...
Christian Schlieker,Hanswalter Zentgraf,Petra Dersch et al.
Christian Schlieker et al.
Hsp100/Clp proteins are key players in the protein quality control network of prokaryotic cells and function in the degradation and refolding of misfolded or aggregated proteins. Here we report the identification of a new class of Hsp100/Cl...
Monitoring the real-time kinetics of the hydrolysis reaction of guanine nucleotide-binding proteins [0.03%]
Alexander Eberth,Radovan Dvorsky,Christian F W Becker et al.
Alexander Eberth et al.
The conversion of guanosine triphosphate (GTP) to guanosine diphosphate (GDP) and inorganic phosphate (Pi) by guanine nucleotide-binding proteins (GNBPs) is a fundamental enzyme reaction in living cells that acts as an important timer in a ...
Sven Vopel,Hermine Mühlbach,Arne Skerra
Sven Vopel
The anticalin FluA is an artificial lipocalin with novelspecificity for the fluorescein group, which was engineered from an insect bilin-binding protein by targeted random mutagenesis and selection. Based on the crystal structure of FluA, a...
Tobias Lamkemeyer,Rüdiger J Paul,Walter Stöcker et al.
Tobias Lamkemeyer et al.
The haemoglobin (Hb) of Daphnia magna acclimated to different oxygen conditions was sampled, and in its natively assembled state it was separated by chromatofocusing. The Hb isoforms were analysed for their subunit composition under denatur...