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Monitoring the real-time kinetics of the hydrolysis reaction of guanine nucleotide-binding proteins

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The conversion of guanosine triphosphate (GTP) to guanosine diphosphate (GDP) and inorganic phosphate (Pi) by guanine nucleotide-binding proteins (GNBPs) is a fundamental enzyme reaction in living cells that acts as an important timer in a variety of biologica... ...