Adam A Golabek,Elizabeth Kida
Adam A Golabek
The lysosomal lumen contains numerous acidic hydrolases involved in the degradation of carbohydrates, lipids, proteins, and nucleic acids, which are basic cell components that turn over continuously within the cell and/or are ingested from ...
Proteolytic mechanism of a novel mitochondrial and chloroplastic PreP peptidasome [0.03%]
一种新型线粒体和叶绿体PreP肽酶的蛋白水解机制研究
Shashi Bhushan,Kenneth A Johnson,Therese Eneqvist et al.
Shashi Bhushan et al.
The 2.1-A-resolution crystal structure of the novel mitochondrial and chloroplastic metalloendopeptidase, AtPreP1, revealed a unique peptidasome structure, in which the two halves of the enzyme completely enfold a huge proteolytic cavity. B...
Treatment of MCF-7 cells with taxol and etoposide induces distinct alterations in the expression of apoptosis-related genes BCL2, BCL2L12, BAX, CASPASE-9 and FAS [0.03%]
紫杉醇和依托泊苷处理的MCF-7细胞中凋亡相关基因BCL2，BCL2L12，BAX，CASPASE-9和FAS表达差异分析
Hellinida Thomadaki,Maroulio Talieri,Andreas Scorilas
Hellinida Thomadaki
We studied alterations in the mRNA expression levels of BCL2 (Bcl-2), BCL2L12, BAX, FAS and CASPASE-9 genes in the MCF-7 breast cancer cell line in response to treatment with two anticancer drugs. Cell toxicity was evaluated by the MTT meth...
Single-cell resolution imaging of membrane-anchored hepatitis C virus NS3/4A protease activity [0.03%]
单细胞分辨率成像检测膜结合丙型肝炎病毒NS3/4A蛋白酶活性
Morgan M Martin,François Jean
Morgan M Martin
The study of host and viral membrane-associated proteases has been hampered due to a lack of in vivo assays. We report here the development of a cell-based fluorescence assay for detecting hepatitis C virus (HCV) NS3/4A juxtamembrane protea...
Development of a red-shifted fluorescence-based assay for SARS-coronavirus 3CL protease: identification of a novel class of anti-SARS agents from the tropical marine sponge Axinella corrugata [0.03%]
开发出了一种用于SARS冠状病毒3CL蛋白酶的红色荧光基检测方法：从热带海洋海绵Axinella corrugata中鉴定出一类新的抗SARS试剂类化合物
Pamela Hamill,Derek Hudson,Richard Y Kao et al.
Pamela Hamill et al.
SARS-coronavirus (SARS-CoV) encodes a main protease, 3CLpro, which plays an essential role in the viral life cycle and is currently the prime target for discovering new anti-coronavirus agents. In this article, we report our success in deve...
Production and processing of a recombinant Fasciola hepatica cathepsin B-like enzyme (FhcatB1) reveals potential processing mechanisms in the parasite [0.03%]
重组肝片吸虫半胱氨酸蛋白酶(FhcatB1)的表达纯化及加工处理揭示其在寄生过程中的潜在作用机制
Simone A Beckham,Ruby H-P Law,Peter M Smooker et al.
Simone A Beckham et al.
The liver fluke, Fasciola hepatica, apparently uses a number of cysteine proteases during its life cycle, most likely for feeding, immune evasion and invasion of tissues. A cathepsin B-like enzyme (herein referred to as FhcatB1) appears to ...
Homologous substitution of ACE C-domain regions with N-domain sequences: effect on processing, shedding, and catalytic properties [0.03%]
ACE类似物的同源替换：对加工、脱落和催化特性的影响
Zenda L Woodman,Sylva L U Schwager,Pierre Redelinghuys et al.
Zenda L Woodman et al.
Angiotensin-converting enzyme (ACE) exists as two isoforms: somatic ACE (sACE), comprised of two homologous N and C domains, and testis ACE (tACE), comprised of the C domain only. The N and C domains are both active, but show differences in...
Charles N Pagel,Sutharshani Sivagurunathan,Lay Hoon Loh et al.
Charles N Pagel et al.
Cells responsible for the formation and maintenance of bone express thrombin-responsive members of the protease-activated receptor family of G protein-coupled receptors. Thrombin has been shown to elicit a number of functional responses in ...
C. elegans as a model system to study the function of the COG complex in animal development [0.03%]
利用秀丽隐杆线虫研究COG复合体在动物发育中的功能
Yukihiko Kubota,Kiyoji Nishiwaki
Yukihiko Kubota
The conserved oligomeric Golgi (COG) complex is an octameric protein complex associated with the Golgi apparatus and is required for proper sorting and glycosylation of Golgi resident enzymes and secreted proteins. Although COG complex func...
Michael N G James
Michael N G James
Fungi and viruses encode a variety of peptidases having a plethora of functions. Many fungal peptidases are extracellular and are likely used to degrade proteins in their environment. Viral peptidases are processing enzymes, intimately invo...