L-arginine influences the structure and function of arginase mRNA in Aspergillus nidulans [0.03%]
L-精氨酸影响嗜渗曲霉鸟氨酸酶mRNA的结构和功能
Piotr Borsuk,Anna Przykorska,Karina Blachnio et al.
Piotr Borsuk et al.
Expression of the arginase structural gene (agaA) in Aspergillus nidulans is subject to complex transcriptional and post-transcriptional regulation. Arginase mRNA has a long 5'-UTR sequence. Analysis of this sequence in silico revealed its ...
Activation profiles of the zymogen of aspergilloglutamic peptidase [0.03%]
激活aspergillongo glutamic蛋白酶原的活性谱型
Xiang-Ping Huang,Yutaka Yabuki,Masaki Kojima et al.
Xiang-Ping Huang et al.
Aspergilloglutamic peptidase produced by Aspergillus niger var. macrosporus belongs to the novel glutamic peptidase family. Its zymogen is autocatalytically activated under acidic conditions to the mature enzyme with a two-chain structure. ...
The insect metalloproteinase inhibitor gene of the lepidopteran Galleria mellonella encodes two distinct inhibitors [0.03%]
家蚕血凝因子基因编码两种不同的金属蛋白酶抑制剂蛋白质
Marianne Wedde,Christoph Weise,Rolf Nuck et al.
Marianne Wedde et al.
The insect metalloproteinase inhibitor (IMPI) from the greater wax moth, Galleria mellonella, represents the first and to date only specific inhibitor of microbial metalloproteinases reported from animals. Here, we report on the characteriz...
Binding of aflatoxins to the 20S proteasome: effects on enzyme functionality and implications for oxidative stress and apoptosis [0.03%]
黄曲霉毒素与20S蛋白酶体的结合：对酶功能的影响及其对氧化应激和凋亡的意义
Manila Amici,Valentina Cecarini,Assuntina Pettinari et al.
Manila Amici et al.
Aflatoxins (AF) are contaminants of improperly stored foods; they are potent genotoxic and carcinogenic compounds, exerting their effects through damage to DNA. They can also induce mutations that increase oxidative damage. The goal of this...
Tissue-specific transcription factor HNF4alpha inhibits cell proliferation and induces apoptosis in the pancreatic INS-1 beta-cell line [0.03%]
组织特异性转录因子HNF4α通过诱导凋亡和抑制增殖来抑制胰腺β细胞系INS-1细胞的生长
Silke Erdmann,Sabine Senkel,Tanja Arndt et al.
Silke Erdmann et al.
Hepatocyte nuclear factor 4alpha (HNF4alpha) is a tissue-specific transcription factor expressed in many cell types, including pancreatic beta-cells. Mutations in the HNF4alpha gene in humans give rise to maturity-onset diabetes of the youn...
Interaction of the cellular prion protein with raft-like lipid membranes [0.03%]
细胞型朊病毒蛋白与类似脂筏的脂质膜相互作用
Kerstin Elfrink,Luitgard Nagel-Steger,Detlev Riesner
Kerstin Elfrink
Conversion of the cellular isoform of the prion protein (PrP(C)) into the disease-associated isoform (PrP(Sc)) plays a key role in the development of prion diseases. Within its cellular pathway, PrP(C) undergoes several posttranslational mo...
Metal-binding sites at the active site of restriction endonuclease BamHI can conform to a one-ion mechanism [0.03%]
金属结合位点在限制性内切核酸酶BamHI活性部位通过单一离子机制起作用
Letif Mones,István Simon,Monika Fuxreiter
Letif Mones
The number of metal ions required for phosphoryl transfer in restriction endonucleases is still an unresolved question in molecular biology. The two Ca(2+) and Mn(2+) ions observed in the pre- and post-reactive complexes of BamHI conform to...
Katja Gotthardt,Mohammad Reza Ahmadian
Katja Gotthardt
Asef is a member of the Dbl-family of guanine nucleotide exchange factors (GEFs) with a proposed specificity for the small GTPase Rac1. Here we investigated the specificity and regulation of Asef by measuring its GEF activity in vitro and o...
Glycine-assisted enhancement of 1,4-beta-d-xylan xylanohydrolase activity at alkaline pH with a pH optimum shift [0.03%]
甘氨酸辅助增强1,4-β-D-木糖烷 xylohydrolase在碱性pH下的活性并使最适pH发生转移
Vinod Vathipadiekal,Anamika Verma,Mala Rao
Vinod Vathipadiekal
This is the first report describing the enhancement of xylanase activity by the neutral amino acid glycine. Xylanase activity is increased seven-fold at alkaline pH in the presence of glycine and its pH optimum is shifted from pH 7 to 8 wit...
Conformational studies on Arabidopsis sulfurtransferase AtStr1 with spectroscopic methods [0.03%]
阿拉伯芥硫转移酶AtStr1的构象研究（光谱法）
Andrea Bartels,Fabio Forlani,Silvia Pagani et al.
Andrea Bartels et al.
Sulfurtransferases/rhodaneses (Str) are enzymes widely distributed in archaea, prokaryota and eukaryota, and catalyze the transfer of sulfur from a donor molecule to a thiophilic acceptor substrate. In this reaction, Str cycles between the ...