Metal-binding sites at the active site of restriction endonuclease BamHI can conform to a one-ion mechanism
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The number of metal ions required for phosphoryl transfer in restriction endonucleases is still an unresolved question in molecular biology. The two Ca(2+) and Mn(2+) ions observed in the pre- and post-reactive complexes of BamHI conform to the classical two-m... ...
