On the presence of C2-ceramide in mammalian tissues: possible relationship to etherphospholipids and phosphorylation by ceramide kinase [0.03%]
关于C2-鞘氨醇在哺乳动物组织中的存在：与醚型磷脂和由鞘氨醇激酶催化的磷酸化作用的可能关系
Helena Van Overloop,Yves Denizot,Myriam Baes et al.
Helena Van Overloop et al.
C(2)-ceramide (N-acetyl-sphingenine) is often used as an analog to study ceramide-mediated cellular processes. According to Lee et al. [J. Biol. Chem. 271 (1996), 209-217], C(2)-ceramide is formed by an acetyl transfer from platelet-activat...
Raft association and lipid droplet targeting of flotillins are independent of caveolin [0.03%]
raft聚集和flotillin的脂滴定位独立于caveolin
Lawrence Rajendran,Soazig Le Lay,Harald Illges
Lawrence Rajendran
Lipid rafts are liquid ordered platforms that dynamically compartmentalize membranes. Caveolins and flotillins constitute a group of proteins that are enriched in these domains. Caveolin-1 has been shown to be an essential component of cave...
Properties and partial purification of sialate-O-acetyltransferase from bovine submandibular glands [0.03%]
牛下颌腺岩藻糖基-O-乙酰转移酶的性质及部分纯化
L Aicha Lrhorfi,G Vinayaga Srinivasan,Roland Schauer
L Aicha Lrhorfi
The O-acetylation of sialic acids in various positions is a frequent modification of these residues in glycoproteins and glycolipids of higher animals and some bacteria. Sialic acid O-acetylation is involved in the regulation of many cell b...
A highly conserved protein secreted by the prostate cancer cell line PC-3 is expressed in benign and malignant prostate tissue [0.03%]
一种由前列腺癌细胞系pc-3分泌的高度保守的蛋白质在良性及恶性前列腺组织中表达
Camilla Valtonen-André,Anders Bjartell,Rebecka Hellsten et al.
Camilla Valtonen-André et al.
In this study we characterize a novel gene on human chromosome 9 and its translation product, PC3-secreted microprotein (PSMP). The gene contains three exons that encode a protein of 139 amino acid residues, including a predicted signal pep...
Maximal Ca2+i stimulation of cardiac Na+/Ca2+ exchange requires simultaneous alkalinization and binding of PtdIns-4,5-P2 to the exchanger [0.03%]
心肌Na+/Ca2+交换器介导的钙离子刺激需要磷脂PtdIns-4, 5-P2与该交换器结合和细胞碱化同时存在
Velia Posada,Luis Beaugé,Graciela Berberián
Velia Posada
Using bovine heart sarcolemma vesicles we studied the effects of protons and phosphatidylinositol-4,5-bisphosphate (PtdIns-4,5-P2) on the affinity of the mammalian Na(+)/Ca(2+) exchanger (NCX1) for intracellular Ca(2+). By following the eff...
Purification, characterization, and molecular gene cloning of an antifungal protein from Ginkgo biloba seeds [0.03%]
银杏种子抗真菌蛋白的分离纯化、鉴定及分子克隆
Yoriko Sawano,Takuya Miyakawa,Hiroshi Yamazaki et al.
Yoriko Sawano et al.
A novel basic protein with antifungal activity was isolated from the seeds of Ginkgo biloba and purified to homogeneity. The protein inhibited the growth of some fungi (Fusarium oxysporum, Trichoderma reesei, and Candida albicans) but did n...
Characterization of the large subunit of EcoHK31I methyltransferase by structural modeling and mutagenesis [0.03%]
EcoHK31I甲基转移酶大亚基的结构建模与点突变表征研究表明
Amanda N-S Mak,Wai-To Fung,Kathy P-S Kong et al.
Amanda N-S Mak et al.
M.EcoHK31I is a naturally occurring mC5-methyltransferase with a large alpha polypeptide and a small beta polypeptide. Polypeptide alpha contains conserved motifs I-VIII and X, and polypeptide beta contains motif IX. To understand how polyp...
Evolutionary selection pressure and family relationships among connexin genes [0.03%]
连接蛋白基因的进化选择压力及其家族关系
Véronique Cruciani,Svein-Ole Mikalsen
Véronique Cruciani
We suggest an extension of connexin orthology relationships across the major vertebrate lineages. We first show that the conserved domains of mammalian connexins (encoding the N-terminus, four transmembrane domains and two extracellular loo...
Cysteine protease inhibitors effectively reduce in vivo levels of brain beta-amyloid related to Alzheimer's disease [0.03%]
半胱氨酸蛋白酶抑制剂有效降低与阿尔茨海默病相关的脑内β-淀粉样蛋白的含量
Vivian Hook,Mark Kindy,Gregory Hook
Vivian Hook
Abnormal accumulation of neurotoxic beta-amyloid peptides (Abeta) in brain represents a key factor in the progression of Alzheimer's disease (AD). Identification of small molecules that effectively reduce brain levels of Abeta is important ...
The His-Pro-Phe motif of angiotensinogen is a crucial determinant of the substrate specificity of renin [0.03%]
血管紧张素原的His-Pro-Phe基序是肾素底物特异性的关键决定因素
Tsutomu Nakagawa,Jyunji Akaki,Ryousuke Satou et al.
Tsutomu Nakagawa et al.
The amino acid sequence His-Pro-Phe as N-terminal residues 6-8 of the natural renin substrate, angiotensinogen, is conserved among species. We investigated whether this His-Pro-Phe motif functions as the determinant of the substrate specifi...