Transcriptional responses of Plasmodium falciparum to alpha-difluoromethylornithine-induced polyamine depletion [0.03%]
α-差氟甲基鸟氨酸诱导的多胺消耗对疟原虫转录的影响
Katherine Clark,Minishca Dhoogra,Abraham I Louw et al.
Katherine Clark et al.
Abstract Polyamines are essential polycationic molecules involved in multiple cellular events, including cell differentiation, division and death. Inhibition of polyamine biosynthesis has been considered in diverse therapeutic strategies ra...
Functional tyrosine residue in the active center of human dipeptidyl peptidase III [0.03%]
人二肽基肽酶III活性中心的功能酪氨酸残基
Branka Salopek-Sondi,Bojana Vukelić,Jasminka Spoljarić et al.
Branka Salopek-Sondi et al.
Abstract Human dipeptidyl peptidase III (DPP III) is a member of the metallopeptidase family M49 with an implied role in the pain-modulatory system and endogenous defense against oxidative stress. Here, we report the heterologous expression...
Effects of gastric inhibitory polypeptide (GIP) and related analogues on glucagon release at normo- and hyperglycaemia in Wistar rats and isolated islets [0.03%]
胃抑制多肽(GIP)及其相关类似物在正常血糖和高血糖情况下对胰高糖素释放的影响：温带大鼠及分离出的胰岛上的作用
Roslyn S Cassidy,Nigel Irwin,Peter R Flatt
Roslyn S Cassidy
Glucose-dependent insulinotropic polypeptide (GIP) is an incretin hormone secreted by endocrine K-cells in response to nutrient absorption. This study has utilised numerous well-characterised dipeptidyl peptidase IV-resistant GIP analogues ...
Evaluation of the 'side door' in carboxylesterase-mediated catalysis and inhibition [0.03%]
评估羧酸酯酶介导催化和抑制作用中的“侧门”效应
Timothy M Streit,Abdolsamad Borazjani,Shellaine E Lentz et al.
Timothy M Streit et al.
Abstract Structures of mammalian carboxylesterases (CEs) reveal the presence of a 'side door' that is proposed to act as an alternative pore for the trafficking of substrates and products. p-Nitrobenzyl esterase (pnb CE) from Bacillus subti...
Secondary structures and functional requirements for thiM riboswitches from Desulfovibrio vulgaris, Erwinia carotovora and Rhodobacter spheroides [0.03%]
脱硫弧菌、欧文氏胡萝卜亚种和红螺菌的thiM核糖调节元件的二级结构及功能要求
Andrea Rentmeister,Günter Mayer,Nicole Kuhn et al.
Andrea Rentmeister et al.
Abstract Bacterial thiM riboswitches contain aptamer domains that bind the metabolite thiamine pyrophosphate (TPP). Binding of TPP to the aptamer domain induces structural rearrangements that are relayed to the expression domain, thereby in...
Development of a human adipocyte model derived from human mesenchymal stem cells (hMSC) as a tool for toxicological studies on the action of TCDD [0.03%]
开发源自人类间充质干细胞（hMSC）的人类脂肪细胞模型作为TCDD作用毒理学研究的工具
Wen Li,Christoph F A Vogel,Phillip Fujiyoshi et al.
Wen Li et al.
Abstract Efforts were made to develop a human adipocyte model that is useful for toxicological studies in vitro. For this purpose, a stem cell line derived from human bone marrow cells, originally from an adult, was induced to differentiate...
Cloning, expression and characterization of insulin-degrading enzyme from tomato (Solanum lycopersicum) [0.03%]
番茄胰岛素降解酶的克隆、表达及特性分析
Yoann Huet,Jochen Strassner,Andreas Schaller
Yoann Huet
A cDNA encoding insulin-degrading enzyme (IDE) was cloned from tomato (Solanum lycopersicum) and expressed in Escherichia coli in N-terminal fusion with glutathione S-transferase. GST-SlIDE was characterized as a neutral thiol-dependent met...
Inhibition of human mu-calpain by conformationally constrained calpastatin peptides [0.03%]
构象约束型钙蛋白酶素肽对人μ-钙蛋白酶的抑制作用
José Pfizer,Irmgard Assfalg-Machleidt,Werner Machleidt et al.
José Pfizer et al.
The 27-mer peptide CP1B-[1-27] derived from exon 1B of calpastatin stands out among the known inhibitors for mu- and m-calpain due to its high potency and selectivity. By systematical truncation, a 20-mer peptide, CP1B-[4-23], was identifie...
Heparinase selectively sheds heparan sulphate from the endothelial glycocalyx [0.03%]
肝素酶选择性地从内皮糖蛋白层脱落硫酸乙酰肝素
Daniel Chappell,Matthias Jacob,Markus Rehm et al.
Daniel Chappell et al.
A healthy vascular endothelium is coated by the endothelial glycocalyx. Its main constituents are transmembrane syndecans and bound heparan sulphates. This structure maintains the physiological endothelial permeability barrier and prevents ...
Employing Rhodobacter sphaeroides to functionally express and purify human G protein-coupled receptors [0.03%]
利用Rhodobacter sphaeroides表达和纯化人源G蛋白偶联受体
Ankita Roy,Arun Kumar Shukla,Winfried Haase et al.
Ankita Roy et al.
G protein-coupled receptors (GPCRs) represent the largest class of cell surface receptors and play crucial roles in many cellular and physiological processes. Functional production of recombinant GPCRs is one of the main bottlenecks to obta...