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Cloning, expression and characterization of insulin-degrading enzyme from tomato (Solanum lycopersicum)

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A cDNA encoding insulin-degrading enzyme (IDE) was cloned from tomato (Solanum lycopersicum) and expressed in Escherichia coli in N-terminal fusion with glutathione S-transferase. GST-SlIDE was characterized as a neutral thiol-dependent metallopeptidase with i... ...