Structural and functional evidence for membrane docking and disruption sites on phospholipase A2-like proteins revealed by complexation with the inhibitor suramin [0.03%]
与抑制剂Suramin结合的磷脂酶A2样蛋白的膜对接和破坏位点的结构和功能证据
Guilherme H M Salvador,Thiago R Dreyer,Walter L G Cavalcante et al.
Guilherme H M Salvador et al.
Local myonecrosis resulting from snakebite envenomation is not efficiently neutralized by regular antivenom administration. This limitation is considered to be a significant health problem by the World Health Organization. Phospholipase A2-...
Structural and functional studies of a metallo-β-lactamase unveil a new type of structurally encoded nickel-containing heterodinuclear site [0.03%]
结构和功能研究揭示了一种新的镍结合异二核位点金属-β-内酰胺酶的结构与功能研究揭露了一种新型结构性编码镍结合异二核站点
Hwajung Choi,Hee Jung Kim,Atsushi Matsuura et al.
Hwajung Choi et al.
The selection of correct metal ions with high fidelity against competing cellular cations is crucial for the function of many metalloenzymes; however, the understanding of the principles that govern metal selectivity is still incomplete. In...
Crystallizing the 6S and 8S spliceosomal assembly intermediates: a complex project [0.03%]
六/八元剪接体组装中间态的纯化与重构
Jann Patrick Pelz,Hermann Schindelin,Katharina van Pee et al.
Jann Patrick Pelz et al.
The small nuclear ribonucleoproteins (snRNPs) U1, U2, U4/6 and U5 are major constituents of the pre-mRNA processing spliceosome. They contain a common RNP core that is formed by the ordered binding of Sm proteins onto the single-stranded Sm...
Nicotinamide mononucleotide adenylyltransferase displays alternate binding modes for nicotinamide nucleotides [0.03%]
尼克酸单核苷酸腺苷酰转移酶显示了烟酸核苷酸的交替结合模式
Roland Pfoh,Emil F Pai,Vivian Saridakis
Roland Pfoh
Nicotinamide mononucleotide adenylyltransferase (NMNAT) catalyzes the biosynthesis of NAD(+) and NaAD(+). The crystal structure of NMNAT from Methanobacterium thermoautotrophicum complexed with NAD(+) and SO4(2-) revealed the active-site re...
Acoustic vibrations contribute to the diffuse scatter produced by ribosome crystals [0.03%]
声振动有助于核糖体晶体产生的散射光信号中的漫散射成分
Yury S Polikanov,Peter B Moore
Yury S Polikanov
The diffuse scattering pattern produced by frozen crystals of the 70S ribosome from Thermus thermophilus is as highly structured as it would be if it resulted entirely from domain-scale motions within these particles. However, the qualitati...
Gino L Turra,Romina B Agostini,Carolina M Fauguel et al.
Gino L Turra et al.
The glyoxalase system is ubiquitous among all forms of life owing to its central role in relieving the cell from the accumulation of methylglyoxal, a toxic metabolic byproduct. In higher plants, this system is upregulated under diverse meta...
Structural basis for the sequestration of the anti-σ(70) factor Rsd from σ(70) by the histidine-containing phosphocarrier protein HPr [0.03%]
含组氨酸磷载体蛋白HPr使σ(70)与抗-σ(70)因子Rsd相互隔离的机制及其结构基础
Young Ha Park,Si Hyeon Um,Saemee Song et al.
Young Ha Park et al.
Histidine-containing phosphocarrier protein (HPr) is a general component of the bacterial phosphoenolpyruvate:sugar phosphotransferase system (PTS) involved in the phosphorylation-coupled transport of numerous sugars called PTS sugars. HPr ...
A high-transparency, micro-patternable chip for X-ray diffraction analysis of microcrystals under native growth conditions [0.03%]
一种高透明、可微图案化的芯片，用于在原始生长条件下对微晶体进行X射线衍射分析
Thomas D Murray,Artem Y Lyubimov,Craig M Ogata et al.
Thomas D Murray et al.
Microcrystals present a significant impediment to the determination of macromolecular structures by X-ray diffraction methods. Although microfocus synchrotron beamlines and X-ray free-electron lasers (XFELs) can enable the collection of int...
Response from Tanley et al. to Crystallography and chemistry should always go together: a cautionary tale of protein complexes with cisplatin and carboplatin [0.03%]
塔纳利等人的回应：晶体学和化学密不可分——顺铂和卡铂蛋白复合物的一个警示故事
Simon W M Tanley,Kay Diederichs,Loes M J Kroon-Batenburg et al.
Simon W M Tanley et al.