Beatriz Herguedas,Isaias Lans,María Sebastián et al.
Beatriz Herguedas et al.
Riboflavin kinases (RFKs) catalyse the phosphorylation of riboflavin to produce FMN. In most bacteria this activity is catalysed by the C-terminal module of a bifunctional enzyme, FAD synthetase (FADS), which also catalyses the transformati...
Native sulfur/chlorine SAD phasing for serial femtosecond crystallography [0.03%]
用于序列飞秒衍射的天然硫/氯SAD相位法
Takanori Nakane,Changyong Song,Mamoru Suzuki et al.
Takanori Nakane et al.
Serial femtosecond crystallography (SFX) allows structures to be determined with minimal radiation damage. However, phasing native crystals in SFX is not very common. Here, the structure determination of native lysozyme from single-waveleng...
Serial crystallographic analysis of protein isomorphous replacement data from a mixture of native and derivative microcrystals [0.03%]
系列晶体学分析蛋白同形替换混合数据的微晶原体和衍生体
Tao Zhang,Deqiang Yao,Jiawei Wang et al.
Tao Zhang et al.
A post-experimental identification/purification procedure similar to that described in Zhang et al. [(2015), IUCrJ, 2, 322-326] has been proposed for use in the treatment of multiphase protein serial crystallography (SX) diffraction snapsho...
The first crystal structure of the peptidase domain of the U32 peptidase family [0.03%]
U32肽酶家族肽酶结构域的首个晶体结构
Magdalena Schacherl,Angelika A M Montada,Elena Brunstein et al.
Magdalena Schacherl et al.
The U32 family is a collection of over 2500 annotated peptidases in the MEROPS database with unknown catalytic mechanism. They mainly occur in bacteria and archaea, but a few representatives have also been identified in eukarya. Many of the...
Atomic resolution crystal structure of Sapp2p, a secreted aspartic protease from Candida parapsilosis [0.03%]
白色念珠菌分泌型天冬氨酸蛋白酶Sap2p的原子分辨率晶体结构
Jiří Dostál,Adam Pecina,Olga Hrušková-Heidingsfeldová et al.
Jiří Dostál et al.
The virulence of the Candida pathogens is enhanced by the production of secreted aspartic proteases, which therefore represent possible targets for drug design. Here, the crystal structure of the secreted aspartic protease Sapp2p from Candi...
Structural characterization of a mitochondrial 3-ketoacyl-CoA (T1)-like thiolase from Mycobacterium smegmatis [0.03%]
来自鸟分支杆菌的线粒体3-酮酰辅酶A(thiolase，T1)样蛋白的结构研究
Neelanjana Janardan,Rajesh K Harijan,Tiila Riikka Kiema et al.
Neelanjana Janardan et al.
Thiolases catalyze the degradation and synthesis of 3-ketoacyl-CoA molecules. Here, the crystal structures of a T1-like thiolase (MSM-13 thiolase) from Mycobacterium smegmatis in apo and liganded forms are described. Systematic comparisons ...
Sequence-dependent structural changes in a self-assembling DNA oligonucleotide [0.03%]
DNA寡核苷酸自组装中的序列依赖性结构变化
Maithili Saoji,Paul J Paukstelis
Maithili Saoji
DNA has proved to be a remarkable molecule for the construction of sophisticated two-dimensional and three-dimensional architectures because of its programmability and structural predictability provided by complementary Watson-Crick base pa...
Octameric structure of Staphylococcus aureus enolase in complex with phosphoenolpyruvate [0.03%]
金黄色葡萄球菌烯醇酶与磷酸烯醇式丙酮酸复合物的八聚体结构
Yunfei Wu,Chengliang Wang,Shenglong Lin et al.
Yunfei Wu et al.
Staphylococcus aureus is a Gram-positive bacterium with strong pathogenicity that causes a wide range of infections and diseases. Enolase is an evolutionarily conserved enzyme that plays a key role in energy production through glycolysis. A...
Crystal structure and kinetic studies of a tetrameric type II β-carbonic anhydrase from the pathogenic bacterium Vibrio cholerae [0.03%]
霍乱病菌中四聚体的II型β-碳酸酐酶的晶体结构和动力学研究
Marta Ferraroni,Sonia Del Prete,Daniela Vullo et al.
Marta Ferraroni et al.
Carbonic anhydrase (CA) is a zinc enzyme that catalyzes the reversible conversion of carbon dioxide to bicarbonate (hydrogen carbonate) and a proton. CAs have been extensively investigated owing to their involvement in numerous physiologica...
Structure-function relationships in Gan42B, an intracellular GH42 β-galactosidase from Geobacillus stearothermophilus [0.03%]
地芽胞杆菌的细胞内GH42β-半乳糖苷酶Gan42B的结构与功能关系
Hodaya V Solomon,Orly Tabachnikov,Shifra Lansky et al.
Hodaya V Solomon et al.
Geobacillus stearothermophilus T-6 is a Gram-positive thermophilic soil bacterium that contains a battery of degrading enzymes for the utilization of plant cell-wall polysaccharides, including xylan, arabinan and galactan. A 9.4 kb gene clu...