Structures of yeast peroxisomal Δ(3),Δ(2)-enoyl-CoA isomerase complexed with acyl-CoA substrate analogues: the importance of hydrogen-bond networks for the reactivity of the catalytic base and the oxyanion hole [0.03%]
酵母过氧化物酶体Δ(3)，Δ(2)烯酰辅酶a异构酶与acyl-coa底物类似物配合物的结构：氢键网络对催化碱和超离子洞反应性的重要性
Goodluck U Onwukwe,M Kristian Koski,Petri Pihko et al.
Goodluck U Onwukwe et al.
Δ(3),Δ(2)-Enoyl-CoA isomerases (ECIs) catalyze the shift of a double bond from 3Z- or 3E-enoyl-CoA to 2E-enoyl-CoA. ECIs are members of the crotonase superfamily. The crotonase framework is used by many enzymes to catalyze a wide range of...
Miroslaw Tarnawski,Thomas R M Barends,Ilme Schlichting
Miroslaw Tarnawski
Cyclic di-GMP is a bacterial second messenger that is involved in switching between motile and sessile lifestyles. Given the medical importance of biofilm formation, there has been increasing interest in understanding the synthesis and degr...
Deformable complex network for refining low-resolution X-ray structures [0.03%]
用于改进低分辨率X射线结构的可变形复杂网络
Chong Zhang,Qinghua Wang,Jianpeng Ma
Chong Zhang
In macromolecular X-ray crystallography, building more accurate atomic models based on lower resolution experimental diffraction data remains a great challenge. Previous studies have used a deformable elastic network (DEN) model to aid in l...
Structure determination of uracil-DNA N-glycosylase from Deinococcus radiodurans in complex with DNA [0.03%]
辐射耐性菌Deinococcus radiodurans的尿嘧啶-DNA N-糖基化酶与DNA复合物结构测定
Hege Lynum Pedersen,Kenneth A Johnson,Colin E McVey et al.
Hege Lynum Pedersen et al.
Uracil-DNA N-glycosylase (UNG) is a DNA-repair enzyme in the base-excision repair (BER) pathway which removes uracil from DNA. Here, the crystal structure of UNG from the extremophilic bacterium Deinococcus radiodurans (DrUNG) in complex wi...
Structural basis for amino-acid recognition and transmembrane signalling by tandem Per-Arnt-Sim (tandem PAS) chemoreceptor sensory domains [0.03%]
串联型Per-Arnt-Sim(串联型PAS)化学感受器感觉域识别氨基酸及跨膜信号转导的结构基础
Yu C Liu,Mayra A Machuca,Simone A Beckham et al.
Yu C Liu et al.
Chemotaxis, mediated by methyl-accepting chemotaxis protein (MCP) receptors, plays an important role in the ecology of bacterial populations. This paper presents the first crystallographic analysis of the structure and ligand-induced confor...
The novel double-folded structure of d(GCATGCATGC): a possible model for triplet-repeat sequences [0.03%]
d(GCATGCATGC)的新型双折叠结构：三联重复序列的一种可能模型
Arunachalam Thirugnanasambandam,Selvam Karthik,Pradeep Kumar Mandal et al.
Arunachalam Thirugnanasambandam et al.
The structure of the decadeoxyribonucleotide d(GCATGCATGC) is presented at a resolution of 1.8 Å. The decamer adopts a novel double-folded structure in which the direction of progression of the backbone changes at the two thymine residues....
Structure of RC1339/APRc from Rickettsia conorii, a retropepsin-like aspartic protease [0.03%]
康拉姆立克次体的RC1339/APRc结构是一种类似逆转丝氨酸蛋白酶的天冬氨酸蛋白酶
Mi Li,Alla Gustchina,Rui Cruz et al.
Mi Li et al.
The crystal structures of two constructs of RC1339/APRc from Rickettsia conorii, consisting of either residues 105-231 or 110-231 followed by a His tag, have been determined in three different crystal forms. As predicted, the fold of a mono...
Complete epitopes for vaccine design derived from a crystal structure of the broadly neutralizing antibodies PGT128 and 8ANC195 in complex with an HIV-1 Env trimer [0.03%]
基于HIV-1衣壳蛋白包膜三聚体与广谱中和抗体PGT128和8ANC195的复合物晶体结构鉴定疫苗设计中的完全表位
Leopold Kong,Alba Torrents de la Peña,Marc C Deller et al.
Leopold Kong et al.
The HIV-1 envelope gp160 glycoprotein (Env) is a trimer of gp120 and gp41 heterodimers that mediates cell entry and is the primary target of the humoral immune response. Broadly neutralizing antibodies (bNAbs) to HIV-1 have revealed multipl...
Comparison of epsilon- and delta-class glutathione S-transferases: the crystal structures of the glutathione S-transferases DmGSTE6 and DmGSTE7 from Drosophila melanogaster [0.03%]
epsilon和delta类谷胱甘肽S转移酶的比较：果蝇Drosophila melanogaster的谷胱甘肽S转移酶DmGSTE6和DmGSTE7的晶体结构
Michele Scian,Isolde Le Trong,Aslam M A Mazari et al.
Michele Scian et al.
Cytosolic glutathione transferases (GSTs) comprise a large family of enzymes with canonical structures that diverge functionally and structurally among mammals, invertebrates and plants. Whereas mammalian GSTs have been characterized extens...
In vacuo X-ray data collection from graphene-wrapped protein crystals [0.03%]
气相条件下石墨烯包覆蛋白晶体的X射线衍射数据分析
Anna J Warren,Adam D Crawshaw,Jose Trincao et al.
Anna J Warren et al.
The measurement of diffraction data from macromolecular crystal samples held in vacuo holds the promise of a very low X-ray background and zero absorption of incident and scattered beams, leading to better data and the potential for accessi...