Mapping Lipid C=C Location Isomers in Organ Tissues by Coupling Photochemical Derivatization and Rapid Extractive Mass Spectrometry [0.03%]
通过光化学衍生与快速萃取质谱联用技术在器官组织中定位脂质C=C位置异构体
Yuan Su,Xiaoxiao Ma,Jessica Page et al.
Yuan Su et al.
Lipid desaturation plays important roles in biological processes and the disease states. Here, we report a simple but efficient method for mapping unsaturated phospholipids including the spatial distribution of lipid C=C location isomers in...
Solution thermochemistry of concanavalin A tetramer conformers measured by variable-temperature ESI-IMS-MS [0.03%]
变温ESI-IMS-MS法测定刀豆蛋白A四聚体构象体的溶液热化学
Tarick J El-Baba,David E Clemmer
Tarick J El-Baba
Variable-temperature nano-electrospray ionization coupled with ion mobility spectrometry-mass spectrometry is used to investigate the thermal denaturation of the tetrameric protein concanavalin A. As the solution temperature is increased, c...
Proteome Dynamics from Heavy Water Metabolic Labeling and Peptide Tandem Mass Spectrometry [0.03%]
重水代谢标记与肽串联质谱的蛋白质组动力学研究
Ahmad Borzou,Vugar R Sadygov,William Zhang et al.
Ahmad Borzou et al.
Protein homeostasis (proteostasis) is a result of a dynamic equilibrium between protein synthesis and degradation. It is important for healthy cell/organ functioning and is often associated with diseases such as neurodegenerative diseases a...
Synthesis of New S-S and C-C Bonds by Photoinitiated Radical Recombination Reactions in the Gas Phase [0.03%]
气相中光激发自由基重组反应的新S-S和C-C键合成
Lance E Talbert,Xing Zhang,Nathan Hendricks et al.
Lance E Talbert et al.
Photoinitiated radical chemistry has proven to be useful for breaking covalent bonds within many biomolecules in the gas phase. Herein, we demonstrate that radical chemistry is useful for bond synthesis in the gas phase. Single peptides con...
Characterization of applied fields for ion mobility separations in traveling wave based structures for lossless ion manipulations (SLIM) [0.03%]
基于旅行波的结构中用于无损离子操控下的离子淌度分离所需外场特性的研究
Ahmed M Hamid,Aneesh Prabhakaran,Sandilya V B Garimella et al.
Ahmed M Hamid et al.
Ion mobility (IM) is rapidly gaining attention for the separation and analysis of biomolecules due to the ability to distinguish the shapes of ions. However, conventional constant electric field drift tube IM separations have limited resolv...
Top-Down Analysis of Disulfide-Linked Proteins Using Photoinduced Radical Reactions and ET-DDC [0.03%]
基于光诱导自由基反应和ET-DDC的二硫键连接蛋白自上而下分析法研究
Sarju Adhikari,Yu Xia,Scott A McLuckey
Sarju Adhikari
Top-down characterization of proteins via tandem mass spectrometry (MS/MS) can be challenging due to the presence of multiple disulfide bond linkages; which significantly inhibit the backbone cleavage efficiency for the formation of structu...
Protonated N-Alkyl-2-nitroanilines Undergo Intramolecular Oxidation of the Alkyl Chain upon Collisional Activation [0.03%]
碰撞活化下质子化的N-烷基-2-硝基苯胺分子发生 intramolecular oxidation反应生成2-氨基硝基苯产物
Justin Paulose,June Cyriac,George Mathai et al.
Justin Paulose et al.
The collisional activation of protonated N-propyl-2-nitroaniline obtained by electrospray ionization shows two major competitive dissociation pathways: the elimination of the elements of propionic acid, [M + H - C3H6O2]+ to give an m/z 107 ...
Top-Down Protein Identification using a Time-of-Flight Mass Spectrometer and Data Independent Acquisition [0.03%]
基于飞行时间质谱仪依赖数据顶向下蛋白质鉴定
Rajeswari Lakshmanan,Joseph A Loo
Rajeswari Lakshmanan
Top-down mass spectrometry and direct dissociation of gas phase intact proteins have been demonstrated to be a powerful platform for identifying proteins from complex mixtures and for elucidating post-translational modifications (PTMs). Fra...
Two-Dimensional Separation Using High-pH and Low-pH Reversed Phase Liquid Chromatography for Top-down Proteomics [0.03%]
基于反相高效液相色谱的二维分离用于顶视蛋白质组学研究
Zhe Wang,Hongyan Ma,Kenneth Smith et al.
Zhe Wang et al.
Advancements in chromatographic separation are critical to in-depth top-down proteomics of complex intact protein samples. Reversed-phase liquid chromatography is the most prevalent technique for top-down proteomics. However, in cases of hi...
Protonation of Curcumin Triggers Sequential Double Cyclization in the Gas-Phase: An Electrospray Mass Spectrometry and DFT Study [0.03%]
电喷雾质谱及DFT研究姜黄素质子化后气相中连续双环化反应机制
June Cyriac,Justin Paulose,M George et al.
June Cyriac et al.
ESI-protonated natural curcumin (1) undergoes gas-phase cyclization and dissociates via competitive expulsions of 2-methoxy phenol and C4H4O2 (diketene or an isomer). Evidence from mechanistic mass spectrometry and from Density Functional T...