Development of an optimised method for the analysis of human blood plasma samples by atmospheric solids analysis probe mass spectrometry [0.03%]
一种优化的大气固相探针质谱测定人体血浆样本的方法的发展
Annabel S J Eardley-Brunt,Anna Jones,Thomas Mills et al.
Annabel S J Eardley-Brunt et al.
Analysis of small-molecule metabolites in plasma has the potential for development as a clinical diagnostic and prognostic tool. Atmospheric solids analysis probe mass spectrometry (ASAP-MS) is capable of performing rapid metabolite and sma...
Morphology Assessment Enabled by Room-Temperature Soft-Landing in Native MS [0.03%]
基于室温原位软着陆的母体MS形貌评估技术
Zihao Qi,Yu-Fu Lin,Sophie R Harvey et al.
Zihao Qi et al.
Native mass spectrometry (nMS) preserves non-covalent interactions, thereby maintaining native-like protein complex assemblies in the gas phase. Herein, the combination of nMS and room temperature ion soft-landing is assessed as a preparati...
Impact of Supercharging on Top-Down Characterization of Monoclonal Antibodies by Collision Induced Dissociation [0.03%]
质子化对单克隆抗体顶部特征化的影响碰撞诱导解离
Lasini Amunugama,Jared B Shaw
Lasini Amunugama
Native top-down characterization of monoclonal antibodies (mAbs) is an attractive approach due to the narrow charge state distribution of the intact mAb and the spectral decongestion afforded by relatively low charge product ions being spre...
Influence of Acceleration Field and Inlet Temperature on Ion Yields of Small Molecules in Atmospheric Pressure MALDI [0.03%]
加速场及入射气温度对大气压基质辅助激光解吸电离过程中小分子离子产额的影响研究
Eugene Moskovets
Eugene Moskovets
The optimization of the accelerating electric field near a target plate as well as temperature of the inlet capillary in a mass spectrometer is essential for achieving a high ion yield in atmospheric pressure matrix-assisted laser desorptio...
Optimising the choice of normalisation method for use in machine-learning classification of human blood plasma ambient ionisation mass spectra [0.03%]
优化机器学习方法在人类血浆离子化质量谱分类中标准化方法的选择
Annabel S J Eardley-Brunt,Liwen Song;Oxford Acute Myocardial Infarction Study;Oxford Abdominal Aortic Aneurysm Study;Claire Vallance
Annabel S J Eardley-Brunt
Construction of large mass spectrometric data sets usually involves some combination of normalisation, scaling, and transformation of individual mass spectra in order to correct for technical (and sometimes biological) variation. Many diffe...
Mass spectrometry structural analysis of intrinsically disordered phosphoproteins [0.03%]
基于质谱的固有无序磷酸蛋白结构分析
Carter Lantz,Muhammad A Zenaidee,Denise Tran et al.
Carter Lantz et al.
Phosphorylation is a ubiquitous protein modification that is known to play important roles in many biological phenomena including cell signaling, the opening and closing of membrane protein channels, and even triggering of amyloid protein a...
Evaluation of Cleavable Crosslinking for Characterization of Proteoform Structural Differences by Top-Down Mass Spectrometry [0.03%]
可裂解交联在顶部-down质谱表征蛋白质形式结构差异中的评估
Erika A Renbarger,Ian K Webb
Erika A Renbarger
Cleavable crosslinking has traditionally been employed in bottom-up mass spectrometry to elucidate protein structure and protein-protein interactions through identification of peptides bearing characteristic mass adducts. Here, we demonstra...
Structural differentiation of protein charge state conformers via gas-phase ion/ion cross-linking mass spectrometry [0.03%]
基于气相离子/离子交联质谱的蛋白质电荷态构象体的结构区分
Woo-Young Kang,Arup Mondal,Alberto Perez et al.
Woo-Young Kang et al.
Determining structures of multiply charged protein ions in mass spectrometry is critical for understanding the relationship between condensed-phase protein structures found in biological environments and gas-phase structures produced during...
Selecting the Correct Autosampler Vial and Insert to Improve Reproducibility and Accuracy in Mass Spectrometry Analyses [0.03%]
选择正确的自动取样瓶和插入物以提高质量谱分析的重复性和准确性
Ashlee T Falls,Erin S Baker
Ashlee T Falls
Acquiring accurate and reproducible data is crucial for analytical chemists, however, sample storage and manipulation play a large role in achieving high-quality results. Autosampler vials and small-volume inserts are used for sample prepar...
Demonstration of a Nested Electrostatic Linear Ion Trap for Flexibility in Selecting Analyzer Figures-of-Merit [0.03%]
用于选择分析器性能指标的嵌套静电线性离子阱的演示
Jordan M Fritz,Ian J Carrick,Nicholas R Ellin et al.
Jordan M Fritz et al.
An electrostatic linear ion trap (ELIT) is used to trap ions between two ion mirrors with image current detection by central detection electrode. Transformation of the time-domain signal to the frequency-domain via Fourier transform (FT) yi...