Evaluation of Cleavable Crosslinking for Characterization of Proteoform Structural Differences by Top-Down Mass Spectrometry [0.03%]
可裂解交联在顶部-down质谱表征蛋白质形式结构差异中的评估
Erika A Renbarger,Ian K Webb
Erika A Renbarger
Cleavable crosslinking has traditionally been employed in bottom-up mass spectrometry to elucidate protein structure and protein-protein interactions through identification of peptides bearing characteristic mass adducts. Here, we demonstra...
Structural differentiation of protein charge state conformers via gas-phase ion/ion cross-linking mass spectrometry [0.03%]
基于气相离子/离子交联质谱的蛋白质电荷态构象体的结构区分
Woo-Young Kang,Arup Mondal,Alberto Perez et al.
Woo-Young Kang et al.
Determining structures of multiply charged protein ions in mass spectrometry is critical for understanding the relationship between condensed-phase protein structures found in biological environments and gas-phase structures produced during...
Selecting the Correct Autosampler Vial and Insert to Improve Reproducibility and Accuracy in Mass Spectrometry Analyses [0.03%]
选择正确的自动取样瓶和插入物以提高质量谱分析的重复性和准确性
Ashlee T Falls,Erin S Baker
Ashlee T Falls
Acquiring accurate and reproducible data is crucial for analytical chemists, however, sample storage and manipulation play a large role in achieving high-quality results. Autosampler vials and small-volume inserts are used for sample prepar...
Demonstration of a Nested Electrostatic Linear Ion Trap for Flexibility in Selecting Analyzer Figures-of-Merit [0.03%]
用于选择分析器性能指标的嵌套静电线性离子阱的演示
Jordan M Fritz,Ian J Carrick,Nicholas R Ellin et al.
Jordan M Fritz et al.
An electrostatic linear ion trap (ELIT) is used to trap ions between two ion mirrors with image current detection by central detection electrode. Transformation of the time-domain signal to the frequency-domain via Fourier transform (FT) yi...
Spatial mapping of phosphatidylcholine sn-positional isomers using CID of divalent metal complexes in imaging mass spectrometry [0.03%]
基于成像质谱中二价金属配合物CID的磷脂酰胆碱sn构型异构体的空间分布映射研究
Tingting Yan,Zunaira Naeem,Zhongling Liang et al.
Tingting Yan et al.
Phosphatidylcholines (PCs) are the main components of cellular membranes. The high degree of structural heterogeneity leads to significant variations in PC functions and complicates structural characterization. For example, the complex mixt...
An Analytical Evaluation of Tools for Lipid Isomer Differentiation in Imaging Mass Spectrometry [0.03%]
影像质谱法分离分析脂质异构体工具的评估研究进展
Boone M Prentice
Boone M Prentice
Imaging mass spectrometry has emerged as a powerful tool to map the spatial distributions of lipid and metabolites in biological tissues. However, these analyses are challenged by the multitude of isobaric (i.e., same nominal mass) and isom...
Determining β-Monosaccharide Head Group Composition with High-Resolution Cyclic Ion Mobility Separations Coupled to Tandem Mass Spectrometry as a First Step for Unknown Cerebroside Analysis [0.03%]
利用高分辨率循环离子淌度分离与串联质谱测定β-单糖头部组分作为未知脑苷脂分析的第一步
Cameron N Naylor,Gabe Nagy
Cameron N Naylor
Cerebrosides, a class of biologically important lipids, are comprised of a monosaccharide head group along with their ceramide tail. However, their accurate characterization is challenging because of the isomerism in both the tail, from pot...
Stepwise Optimization of Traveling Wave Profiles and Inverse Gating Pattern in Structure for Lossless Ion Manipulation Platform [0.03%]
无损离子操控平台上行波场及逆栅信号的逐步优化
Latif Mohsen,Xi Chen,Gandhi Viraj et al.
Latif Mohsen et al.
The Structure for Lossless Ion Manipulation (SLIM) platform is a powerful analytical separation tool allowing ultra-long serpentine separation path spanning hundreds of meters. In SLIM, transient fields called Traveling waves (T-wave) roll ...
Rapid and Accurate Identification of Microorganisms Using Ion Mobility-Mass Spectrometry [0.03%]
使用离子迁移-质量光谱法快速准确地识别微生物
Ahmed M Hamid
Ahmed M Hamid
Accurate identification of microorganisms to the strain and substrain levels in clinical and environmental samples is essential to provide an appropriate anti-biotherapy to the patients and reduce the prescription of broad-spectrum antimicr...
Kemi E Osho,Keshari Kunwor,Nicholas B Borotto
Kemi E Osho
Free radical-initiated peptide sequencing (FRIPS) is a tandem mass spectrometry technique (MS/MS) that enables radical-based dissociation on instruments only capable of collisional activation. In FRIPS, peptides are chemically-derivatized w...