Enhancing Performance of Liquid Sample Desorption Electrospray Ionization Mass Spectrometry Using Trap and Capillary Columns [0.03%]
利用捕集管和毛细管柱优化液体样品DESI质谱分析性能
Si Cheng,Jun Wang,Yi Cai et al.
Si Cheng et al.
Desorption electrospray ionization mass spectrometry (DESI-MS) is a recent and important advance in the field that has extensive applications in surface analysis of solid samples but has also been extended to analysis of liquid samples. The...
Evaluation of dimethyl sulfoxide (DMSO) as a mobile phase additive during top 3 label-free quantitative proteomics [0.03%]
三甲基二氧化硫(DMSO)作为顶空标签定量蛋白质组学中流动相添加剂的评价研究
Dominika Strzelecka,Stephen W Holman,Claire E Eyers
Dominika Strzelecka
Dimethyl sulfoxide (DMSO) has been advocated as a beneficial additive to electrospray solvents for peptide analysis due to the improved ionisation efficiency conferred. Previous reports have shown that the resultant improvements in peptide ...
Evaluating the use of HILIC in large-scale, multi dimensional proteomics: Horses for courses? [0.03%]
评估HILIC在大规模多维蛋白质组学中的应用：因事任人？
Dalila Bensaddek,Armel Nicolas,Angus I Lamond
Dalila Bensaddek
Despite many recent advances in instrumentation, the sheer complexity of biological samples remains a major challenge in large-scale proteomics experiments, reflecting both the large number of protein isoforms and the wide dynamic range of ...
Protein profiling of mefloquine resistant Plasmodium falciparum using mass spectrometry-based proteomics [0.03%]
基于质谱的蛋白质组学研究抗氯喹恶性疟原虫蛋白谱图
Onrapak Reamtong,Krongkan Srimuang,Naowarat Saralamba et al.
Onrapak Reamtong et al.
Malaria is a mosquito borne infectious disease caused by protozoa of genus Plasmodium. There are five species of Plasmodium that are found to infect humans. Plasmodium falciparum can cause severe malaria leading to higher morbidity and mort...
Systematic analysis of the use of amphipathic polymers for studies of outer membrane proteins using mass spectrometry [0.03%]
两性聚合物在外膜蛋白质谱研究中的系统分析方法学探究
Thomas G Watkinson,Antonio N Calabrese,Fabrice Giusti et al.
Thomas G Watkinson et al.
Membrane proteins (MPs) are essential for numerous important biological processes. Recently, mass spectrometry (MS), coupled with an array of related techniques, has been used to probe the structural properties of MPs and their complexes. T...
Probing Asymmetric Charge Partitioning of Protein Oligomers during Tandem Mass Spectrometry [0.03%]
利用串联质谱研究蛋白质寡聚体解离过程中的不对称电荷分布现象
Philip D Compton,Luca Fornelli,Neil L Kelleher et al.
Philip D Compton et al.
Dissociation of gaseous protein complexes produced by native electrospray often induces an asymmetric partitioning of charge between ejected subunits. We present a simple asymmetric charge partitioning factor (ACPF) to quantify the magnitud...
RADICAL-INDUCED FRAGMENTATION OF PHOSPHOLIPID CATIONS USING METASTABLE ATOM-ACTIVATED DISSOCIATION MASS SPECTROMETRY (MAD-MS) [0.03%]
利用亚稳态原子活化解离质谱(MAD-MS)诱导磷脂阳离子的自由基断裂反应
Robert E Deimler,Madlen Sander,Glen P Jackson
Robert E Deimler
The fragmentation pattern of several protonated 1+ phosphatidylcholines (PCs) were studied using low energy collision induced dissociation (CID) and helium metastable atom-activated dissociation (He-MAD). He-MAD of the protonated compounds ...
Enhancing Protein Disulfide Bond Cleavage by UV Excitation and Electron Capture Dissociation for Top-Down Mass Spectrometry [0.03%]
用于顶部向下质谱的蛋白质二硫键裂解的UV激发和电子捕获解离增强技术
Piriya Wongkongkathep,Huilin Li,Xing Zhang et al.
Piriya Wongkongkathep et al.
The application of ion pre-activation with 266 nm ultraviolet (UV) laser irradiation combined with electron capture dissociation (ECD) is demonstrated to enhance top-down mass spectrometry sequence coverage of disulfide bond containing prot...
Xiang Yu,Nadezda P Sargaeva,Christopher J Thompson et al.
Xiang Yu et al.
Deamidation and the subsequent formation of isoaspartic acid (isoAsp) are common modifications of asparagine (Asn) residues in proteins. Differentiation of isoAsp and Asp residues is a challenging task owing to their similar chemical proper...
Zhou Peng,Scott A McLuckey
Zhou Peng
The formation of peptide bonds is of great importance from both a biological standpoint and in routine organic synthesis. Recent work from our group demonstrated the synthesis of peptides in the gas-phase via ion/ion reactions with sulfo-NH...