Y Kato,S Hiraoka,Y Tada et al.
Y Kato et al.
To improve solid particle suspensions in liquids in a shaking vessel, a pole was installed at the axis of the shaking vessel, which was referred to as the "current pole". The performance of a shaking vessel with current pole at its central ...
Out-of-phase operating conditions, a hitherto unknown phenomenon in shaking bioreactors [0.03%]
离相操作状态:搅拌式生物反应器中此前未被发现的现象
J Büchs,S Lotter,C Milbradt
J Büchs
One of the important parameters in characterising fermentations of aerobic microorganisms is the specific power consumption. A new method has been introduced which enables the accurate determination of the power consumption in shaking biore...
Scale-up of filamentous organisms from tubes and shake-flasks into stirred vessels [0.03%]
丝状菌由试管和摇瓶扩大到搅拌罐中的培养
W Katzer,M Blackburn,K Charman et al.
W Katzer et al.
The choice of small-scale fermentation systems contributes significantly to a successful scale-up. Creasing of flasks and the chosen shaker parameters influence the production of secondary metabolites in a strain- and even compound-specific...
Development of a shaking bioreactor system for animal cell cultures [0.03%]
一种用于动物细胞培养的震荡生物反应器系统的开发研究论文标题翻译
C -M. Liu,L -N. Hong
C -M. Liu
The feasibility of using shake flasks to culture animal cells was evaluated using various sizes of cylindrical shaped vessels as bioreactors. It was found that conditions can be optimized so that hybridoma, Chinese Hamster Ovary cells, and ...
P Girard,M Jordan,M Tsao et al.
P Girard et al.
An agitated 12-well microtiter plate system with a working volume of 2ml was investigated for cell culture process development. Agitation assures homogeneity in wells and enhances mass transfer between the gas and the liquid phase, thus imp...
Effectiveness of orbital shaking for the aeration of suspended bacterial cultures in square-deepwell microtiter plates [0.03%]
方形深孔微滴定板中悬浮细菌培养的通气效果研究
W A. Duetz,B Witholt
W A. Duetz
Growth of heterogeneous culture collections in microtiter plates is advantageous for logistic reasons and also in enabling significant savings in medium costs, labor input and use of equipment during large screening projects. The main hurdl...
C Mrotzek,T Anderlei,H -J. Henzler et al.
C Mrotzek et al.
One of the mass transfer resistances for the gas exchange of shaking flasks is the sterile plug. The gas exchange through the sterile plug is described by an extended model of Henzler and Schedel [Bioprocess Eng. 7 (1991) 123]. Based on thi...
Characterisation of the gas-liquid mass transfer in shaking bioreactors [0.03%]
shake flask中气液传质过程的表征
U Maier,J Büchs
U Maier
The maximum gas-liquid mass transfer capacity of 250ml shaking flasks on orbital shaking machines has been experimentally investigated using the sulphite oxidation method under variation of the shaking frequency, shaking diameter, filling v...
J Büchs
J Büchs
Shaking bioreactors are the most frequently used reaction vessels in biotechnology and have been so for many decades. In spite of their large practical importance, very little is known about the characteristic properties of shaken cultures ...
Editor's preface [0.03%]
编者前言
C Webb,S Furusaki
C Webb