Enhancing CRISPR Homology Directed Repair in IAL-PiD2 Insect Cells via Reagent Delivery Optimization and Cell Synchronization [0.03%]
通过优化试剂递送和细胞同步化提高昆虫IAL-PiD2细胞中的CRISPR同源定向修复效率
Bryce D Shirk,Cecilia Z Rodriguez,Marisa O Pacheco et al.
Bryce D Shirk et al.
CRISPR/Cas9-mediated homology-directed repair (HDR) enables precise genome editing, yet its application in insect cell lines remains largely unexplored. Insect systems, already used in recombinant protein production via baculovirus expressi...
Ross R Klauer,Mekhi Williams,Darien K Nguyen et al.
Ross R Klauer et al.
Biological plastics deconstruction and upcycling have emerged as sustainable alternatives to traditional recycling technologies for plastics waste. The discovery and engineering of efficient thermostable poly(ethylene terephthalate) (PET) h...
An Elastin-like Polymer Targeting Vascular Endothelial Growth Factor Receptor-1 Reduces Survival in Serum-Starved Endothelial Cells [0.03%]
一种针对血管内皮生长因子受体-1的弹性蛋白多肽减少了饥饿状态下内皮细胞的存活率
M Lisa Phipps,Antonietta M Lillo,Demosthenes P Morales et al.
M Lisa Phipps et al.
Peptides often exhibit biological activity that depends on the context in which they are displayed and delivered. Understanding and controlling these contextual effects on peptide function is critical for designing targeted and responsive p...
Soft surfaces induce neural differentiation via the neuron restrictive silencer factor [0.03%]
软基质通过神经限制沉默因子诱导细胞分化成为神经元
Ryan Thompson,Nick Mazur,Jie Yang et al.
Ryan Thompson et al.
The physical and mechanical environment influence the differentiation and culture of stem cells. Early research in this field showed that mesenchymal stem cells (MSCs) adopt preferential lineages when cultured on surfaces of varying stiffne...
The Role of Endoplasmic Reticulum Stress on Reducing Recombinant Protein Production in Mammalian Cells [0.03%]
内质网压力对降低哺乳动物细胞重组蛋白生产的作用
R Chauncey Splichal,Kevin Chen,S Patrick Walton et al.
R Chauncey Splichal et al.
Therapeutic recombinant protein production relies on industrial scale culture of mammalian cells to produce active proteins in quantities sufficient for clinical use. The combination of stresses from industrial cell culture environment and ...
Evaluation of the efficiency of various methods to load fluoroquinolones into Escherichia coli outer membrane vesicles as a novel antibiotic delivery platform [0.03%]
评估各种方法向大肠杆菌外膜囊泡中装载氟喹诺酮类药物的效率以作为新型抗生素递送平台
Meishan Wu,Lauryn Holgado,Rachael M Harrower et al.
Meishan Wu et al.
The development of novel antibacterial agents that are effective against Gram-negative bacteria is limited primarily by transport issues. This class of bacteria maintains a complex cell envelope consisting of two membrane bilayers, preventi...
Engineering Alkaline-Stable Barley Stripe Mosaic Virus-Like Particles for Efficient Surface Modification [0.03%]
用于高效表面修饰的工程型碱性稳定大麦条纹花叶病毒样颗粒
Akash J Vaidya,Mruthula Rammohan,Yu-Hsuan Lee et al.
Akash J Vaidya et al.
Viruses and virus-like particles are powerful templates for materials synthesis because of their capacity for precise protein engineering and diverse surface functionalization. We recently developed a recombinant bacterial expression system...
Incorporating a β-hairpin sequence motif to increase intracellular stability of a peptide-based PROTAC [0.03%]
通过引入β-发夹序列模体提高基于肽的PROTAC胞内稳定性
Hannah C Hymel,Jeffery C Anderson,Dong Liu et al.
Hannah C Hymel et al.
Proteolysis targeting chimeras (PROTACs) have emerged as a new class of therapeutics that utilize the ubiquitin-proteasome system (UPS) to facilitate proteasomal degradation of "undruggable" targets. Peptide-based PROTACs contain three esse...
Effects of Sequence Features on Machine-Learned Enzyme Classification Fidelity [0.03%]
序列特征对机器学习酶分类保真度的影响
Sakib Ferdous,Ibne Farabi Shihab,Nigel F Reuel
Sakib Ferdous
Assigning enzyme commission (EC) numbers using sequence information alone has been the subject of recent classification algorithms where statistics, homology and machine-learning based methods are used. This work benchmarks performance of a...
Bioreactor Expansion Reconfigures Metabolism and Extracellular Vesicle Biogenesis of Human Adipose-derived Stem Cells In Vitro [0.03%]
生物反应器扩增体外重构人脂肪来源干细胞的代谢和细胞外囊泡发生过程
Richard Jeske,Xingchi Chen,Shaoyang Ma et al.
Richard Jeske et al.
Human mesenchymal stem cells (hMSCs), including human adipose tissue-derived stem cells (hASCs), as well as the secreted extracellular vesicles (EVs), are promising therapeutics in treating inflammatory and neural degenerative diseases. How...