The effects of protein solubility on the RNA Integrity Number (RIN) for recombinant Escherichia coli [0.03%]
蛋白质溶解性对重组大肠杆菌RNA完整性数(RIN)的影响
Mary Alice Salazar,Lawrence P Fernando,Faraz Baig et al.
Mary Alice Salazar et al.
High quality, intact messenger RNA (mRNA) is required for DNA microarray and reverse transcriptase polymerase chain reaction analysis and is generally obtained from total RNA isolations. The most widely recognized measure of RNA integrity i...
Reproducible culture and differentiation of mouse embryonic stem cells using an automated microwell platform [0.03%]
小鼠胚胎干细胞培养和定向分化过程中可重复性的研究——微孔板自动化平台的应用
Waqar Hussain,Nathalie Moens,Farlan S Veraitch et al.
Waqar Hussain et al.
The use of embryonic stem cells (ESCs) and their progeny in high throughput drug discovery and regenerative medicine will require production at scale of well characterized cells at an appropriate level of purity. The adoption of automated b...
Nasim Annabi,Suzanne M Mithieux,Gulden Camci-Unal et al.
Nasim Annabi et al.
Elastomeric protein-based biomaterials, produced from elastin derivatives, are widely investigated as promising tissue engineering scaffolds due to their remarkable properties including substantial extensibility, long-term stability, self-a...
Michael C Naill,Martin E Kolewe,Susan C Roberts
Michael C Naill
The transport of paclitaxel in Taxus canadensis suspension cultures was studied with a fluorescence analogue of paclitaxel (Flutax-2(®)) in combination with flow cytometry detection. Experiments were carried out using both isolated protopl...
Self-interaction chromatography of proteins on a microfluidic monolith [0.03%]
微流体单体结构上的蛋白质自我相互作用色谱法
Cristina Martin,Abraham M Lenhoff
Cristina Martin
A novel miniaturized system has been developed for measuring protein-protein interactions in solution with high efficiency and speed, and minimal use of protein. A chromatographic monolith synthesized in a capillary is used in the method to...
Samira M Azarin,Sean P Palecek
Samira M Azarin
The use of human pluripotent stem cells, including embryonic and induced pluripotent stem cells, in therapeutic applications will require the development of robust, scalable culture technologies for undifferentiated cells. Advances made in ...
Cholesterol Supplementation During Production Increases the Infectivity of Retroviral and Lentiviral Vectors Pseudotyped with the Vesicular Stomatitis Virus Glycoprotein (VSV-G) [0.03%]
胆固醇补充增加假病毒型逆转录病毒和慢病毒载体的感染力
Yong Chen,Christopher J Ott,Kay Townsend et al.
Yong Chen et al.
Cholesterol, a major component of plasma membrane lipid rafts, is important for assembly and budding of enveloped viruses, including influenza and HIV-1. Cholesterol depletion impairs virus assembly and infectivity. This study examined the ...
Dynamics of Positional Enrichment: Theoretical Development and Application to Carbon Labeling in Zymomonas mobilis [0.03%]
位置富集的动力学：理论发展及应用于Zymomonas mobilis的碳标记研究
Fernando Alvarez-Vasquez,Yusuf A Hannun,Eberhard O Voit
Fernando Alvarez-Vasquez
Positional enrichment analysis has become an important technique for assessing detailed flux distributions and the fates of specific atoms in metabolic pathway systems. The typical approach to positional enrichment analysis is performed by ...
A modified method for determination of diffusivities of low molecular substances in non-Newtonian liquids [0.03%]
一种测定低分子物质在非牛顿流体中扩散系数的改进方法研究
G Peev,A Nikolova,V Beschkov et al.
G Peev et al.
A modified method for determination of diffusivities of low molecular substances in non-Newtonian liquids described by the power-law model has been proposed. It is based on the dissolution of Geiss body, with a parameter m=1/3 rotating in a...
Carbon sources affect metabolic capacities of Bacillus species for the production of industrial enzymes: theoretical analyses for serine and neutral proteases and alpha-amylase [0.03%]
碳源影响芽孢杆菌属物种生产工业酶的代谢能力：关于丝氨酸和中性蛋白酶及淀粉酶的理论分析
P Çalik,T H. Özdamar
P Çalik
The metabolic fluxes through the central carbon pathways were calculated for the genus Bacillus separately for the enzymes serine alkaline protease (SAP), neutral protease (NP) and alpha-amylase (AMY) on five carbon sources that have differ...