A Cas12a Toolbox for Rapid and Flexible Group B Streptococcus Genomic Editing and CRISPRi [0.03%]
适用于快速灵活的B群链球菌基因组编辑和CRISPRi的Cas12a工具箱
G H Hillebrand,S C Carlin,E J Giacobe et al.
G H Hillebrand et al.
Streptococcus agalactiae (group B Streptococcus; GBS) is a leading cause of neonatal sepsis and meningitis. Despite advances in molecular microbiology, GBS genome engineering remains laborious due to inefficient mutagenesis protocols. Here,...
The Role of the [2Fe-2S] Cluster of Escherichia coli IscR in Responding to Redox-Cycling Agents [0.03%]
脱氧核糖核酸的重组及在大肠杆菌中的表达
Rajdeep Banerjee,Erin L Mettert,Angela S Fleischhacker et al.
Rajdeep Banerjee et al.
The mechanisms by which cells respond to growth inhibitory redox-cycling agents is only partially understood. In Escherichia coli K12, the IscR regulon, which includes the ISC and SUF Fe-S cluster biogenesis machineries, is differentially e...
A Small RNA Derived From the 5' End of the IS200 tnpA Transcript Regulates Multiple Virulence Regulons in Salmonella typhimurium [0.03%]
来源于IS200 tnpA转录本5'-端的小RNA调节沙门氏菌多个毒力调控网络
Ryan S Trussler,Naomi-Jean Q Scherba,Hoda Kooshapour et al.
Ryan S Trussler et al.
The insertion sequence IS200 is widely distributed in Eubacteria. Despite its prevalence, IS200 does not appear to be mobile and as such is considered an ancestral component of bacterial genomes. Previous work in Salmonella enterica reveale...
Defining the Enterococcus faecalis Fatty Acid Kinase System of Exogeneous Fatty Acid Utilization [0.03%]
定义游离脂肪酸利用的外源性脂肪酸激酶系统
Huijuan Dong,Qi Zou,John E Cronan
Huijuan Dong
Phospholipid synthesis in Firmicute bacteria differs markedly from that of the paradigm Escherichia coli pathway in that acyl phosphates are a key intermediate. Acyl phosphates are required for the first acylation step of the phospholipid s...
Homeostatic Influence of Fig4 Outside of the Fab1-Vac14-Fig4 Complex in Saccharomyces cerevisiae [0.03%]
酿酒酵母中Fig4调控脂质代谢的机制研究
Hannah E Reeves,Anna King,Imran Khan et al.
Hannah E Reeves et al.
The lipid phosphatase Fig4 is conserved in all eukaryotes and is associated with human neurological diseases for which there are currently no specific therapies. Fig4 functions in both the production and turnover of its lipid substrate, PI3...
The Monofunctional FMN Riboswitch-Binding Regulator RibR From Bacillus amyloliquefaciens Promotes Riboflavin Biosynthesis [0.03%]
解淀粉芽孢杆菌单功能核糖体开关结合调节因子RibR促进核黄素生物合成
Anna Hübenthal,Matthias Mack
Anna Hübenthal
Flavin mononucleotide (FMN) riboswitches are RNA-based regulatory elements found in many bacteria. FMN riboswitches control genes responsible for the biosynthesis and transport of riboflavin (rib genes). Riboflavin (vitamin B2) is the precu...
Autophagy and the Mitochondrial Lon1 Protease Are Necessary for Botrytis cinerea Heat Adaptation [0.03%]
自噬和线粒体Lon1蛋白酶对葡萄孢菌热适应性是必不可少的
Mingzhe Zhang,Liang Ma,Zhiqun Lyu et al.
Mingzhe Zhang et al.
Heat adaptation is a multilayered universal process involving a coordinated response of general and heat-specific cellular systems and processes. Here, we demonstrate that adaptation of the plant pathogenic fungus Botrytis cinerea to mild h...
Filamentation Profiling Reveals Multiple Transcription Regulators Contributing to the Differences Between Candida albicans and Candida dubliniensis [0.03%]
丝状图谱分析揭示了多种转录调节因子对白假丝酵母和都柏林假丝酵母之间差异的贡献
Teresa Meza-Davalos,Luis F García-Ortega,Eugenio Mancera
Teresa Meza-Davalos
Candida dubliniensis is the most closely related species to C. albicans, one of the leading causes of fungal infections in humans. However, despite sharing many characteristics, C. dubliniensis is significantly less pathogenic. To better un...
Enzymatic and Structural Roles of Candida albicans Rev1 in DNA Damage Response and Disseminated Candidiasis [0.03%]
白念珠菌Rev1在DNA损伤应答和侵袭性念珠菌病中的酶学及结构作用机制研究
Satya Ranjan Sahu,Sushree Subhashree Parida,Bhabasha Gyanadeep Utkalaja et al.
Satya Ranjan Sahu et al.
Translesion DNA synthesis (TLS) is a fundamental biological process that enables DNA replication through various lesions to ensure genome stability and to prevent cell death due to replication fork collapse. Rev1, a member of Y-family DNA p...
Characterization of a Novel RNA Polymerase Mutant of Escherichia coli That Confers Thermotolerance and Chaperone Independence [0.03%]
一个赋予热稳定性和分子伴侣独立性的新型大肠杆菌RNA聚合酶突变体的表征研究
Melody Yeh,Keilen Kelly,Rajeev Misra
Melody Yeh
Bacterial cells activate stress-sensing and stress-mitigating pathways by employing a number of transcription regulators, some of which can bind directly to RNA polymerase to activate stress-specific response pathways. However, mutations in...