Atomic force microscopy: The creator of a new paradigm from nanoscale topography imaging to mechanobiology and medicine [0.03%]
原子力显微镜：从纳米形貌表征到机械生物学与医学的新范例创造者
Qianhui Xu,Huaiwei Zhang,Junmei Chen et al.
Qianhui Xu et al.
Atomic force microscopy (AFM) has transcended its role as a mere provider of high-resolution imaging in medical research, catalysing a paradigm shift from 'morphological observation' to 'quantitative mechanics' and 'single molecule manipula...
A detailed protocol for open and low-cost six-plex immunofluorescence (Flex-6 mIHF) with a proof-of-concept study on breast cancer tissue [0.03%]
一种用于乳腺组织的开放且低成本六重免疫荧光技术（Flex-6 mIHF）的详细实验方案及其概念验证研究
Julia A M Riggi,Aurélie Daumerie,Naïma Benhaddi et al.
Julia A M Riggi et al.
Multiplex immunohistofluorescence (mIHF) allows to investigate protein (co)-localisation in the tumour microenvironment, which can facilitate research on carcinogenesis. Most available technologies for multiplex staining are expensive and i...
Mariana De Niz,Leandro Lemgruber
Mariana De Niz
3SD: Rotational symmetry single-shot denoising in fluorescence microscopy [0.03%]
3SD：荧光显微镜旋转对称性单次去噪法
Tijmen H de Wolf,Pleun Engbers,Justine Perrin et al.
Tijmen H de Wolf et al.
Image noise is a fundamental problem in fluorescence microscopy analysis, especially in live cell imaging applications where the number of detected photons is limited due to low power of excitation lasers to prevent phototoxicity during ext...
Single-molecule validation and optimised protocols for the use of secondary nanobodies in multiplexed immunoassays [0.03%]
单分子验证和用于多重免疫测定中次级纳米抗体使用的优化方案
Rebecca Saleeb,Judi O&#x;Shaughnessy,Ryan Ferguson et al.
Rebecca Saleeb et al.
Recently developed secondary nanobodies or single-domain antibodies present a powerful tool for immunodetection. Unlike traditional antibodies, their monovalence enables pre-association with primary antibodies prior to sample staining, pres...
Multimodal optical microscopy defect detection method for indium bump arrays based on multi-scale Demons deformation field difference [0.03%]
基于多尺度Demons形变场差的铟柱阵列多模态光学显微缺陷检测方法
Yifei Li,Ziyi Wang,Yong Li et al.
Yifei Li et al.
Persistent challenges in indium bump defect detection for infrared focal plane array (IRFPA) include low detection accuracy and high false-positive rates caused by subtle deformations from process variations, imaging distortions and scale v...
Erythrocyte 'Feierzeit' reaction: Novel filamentous and vesicular response to n-butyl acetate [0.03%]
乙酰丁二酸对红细胞的新型丝状和囊泡反应
Philip W Kuchel
Philip W Kuchel
Human erythrocytes (red blood cells; RBCs) undergo spontaneous disassembly after several hours of exposure to n-butyl acetate (nBA). Images of the morphological changes were captured in time-lapse sequences using differential interference c...
From observation to understanding: A multi-agent framework for smart microscopy [0.03%]
从观察到理解：智能显微镜的多主体框架
P S Kesavan,Pontus Nordenfelt
P S Kesavan
Smart microscopy represents a paradigm shift in biological imaging, moving from passive observation tools to active collaborators in scientific inquiry. Enabled by advances in automation, computational power, and artificial intelligence, th...
Exploring poly-L-lysine-based particle capture for atomic force microscopy studies of extracellular vesicles [0.03%]
基于聚L-赖氨酸的颗粒捕获用于原子力显微镜研究外泌体的研究
L Conti,A Ridolfi,A Borup et al.
L Conti et al.
We herein investigate the effects of varying the main experimental variables in one of the most used protocols for extracellular vesicle (EV) immobilisation on substrates for subsequent atomic force microscopy (AFM) quantitative morphometry...
A hybrid model for structured illumination microscopy reconstruction using attention mechanism and deep Laplacian pyramid network with Fourier loss [0.03%]
基于注意力机制和深层拉普拉斯金字塔网络傅里叶损失的结构照明显微镜重建混合模型
Sarfaraj Mirza,Vivek Bohane,Balpreet S Ahluwalia et al.
Sarfaraj Mirza et al.
Structured illumination microscopy (SIM) enables superresolution imaging of biological samples but suffers from artefacts, noise, and loss of high-frequency details in low-light conditions. These problems arise due to limitations in traditi...