Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila [0.03%]
评估表征果蝇组织流动的生物图像分析工作流程的可重复性
Mélodie Ambroset,Cesar Augusto Valades-Cruz,Libert Brice Tonfack et al.
Mélodie Ambroset et al.
Reproducibility challenges in bioimage analysis are common. The proliferation of tools and workflows creates a complex ecosystem that is difficult to reproduce without deliberate, explicit reporting and support. We assess the reproducibilit...
Christian Tischer,Antonio Zaccaria Politi,Tim-Oliver Buchholz et al.
Christian Tischer et al.
Modern microscopy enables us to measure structural and dynamical properties of many biological processes and is therefore an indispensable research tool. However, the amount and complexity of the produced imaging data is steadily increasing...
Evaluating integrative strategies for incorporating phenotypic features in spatial transcriptomics [0.03%]
评估在空间转录组学中整合表型特征的综合策略
Levin M Moser,Ahmad Kamal Hamid,Esteban Miglietta et al.
Levin M Moser et al.
The key advantage of spatial transcriptomics (ST) technologies lies in the spatial domain: these techniques not only offer an unprecedented opportunity to interrogate intact biological samples in a spatially informed manner, but also set th...
20th Anniversary of human-induced pluripotent stem cells and the role of microscopy [0.03%]
诱导多能干细胞诞生二十周年及显微学的作用
Philomena Hallford,Colette J Mustard,Nicole Brace et al.
Philomena Hallford et al.
It has been 20 years since the pioneering work of Shinya Yamanaka and Kazutoshi Takahashi at Kyoto University led to the first successful generation of induced pluripotent stem cells (iPSCs) from mouse embryonic and adult fibroblast cells. ...
Comment on 'A novel experimental platform to monitor solid/water interfaces under freeze-thaw cycles' [0.03%]
关于“一种监测固/水界面冻融循环的新实验平台”的评论
Maajid Mohi Ud Din Malik,Abhay Saraf,Milind P Bhrushundi
Maajid Mohi Ud Din Malik
An affordable fiducial marker strategy for reliable autofocus in long-term live microscopy [0.03%]
一种可靠的长期活体显微镜自动对焦的定位标志策略
Ilyas Djafer-Cherif,Bartlomiej Waclaw
Ilyas Djafer-Cherif
Long-term time-lapse imaging of biological samples requires correcting for focal drift, which would otherwise gradually push the sample out of focus. We present a software-based method that eliminates this time-dependent blur using only a m...
Simplex-based model for nanoparticle grain identification in four-dimensional scanning transmission electron microscopy data [0.03%]
基于单纯形的四维扫描透射电子显微镜纳米晶粒识别模型
Wei Liu,Roberto Dos Reis,Chad A Mirkin et al.
Wei Liu et al.
Grain identification in polycrystalline nanoparticles, for example, determining which crystal phases are present at each spatial location, is fundamental to materials characterisation. This is particularly challenging when grains overlap ex...
Assessing mature and immature collagen in tonsillar tissue using second harmonic generation microscopy [0.03%]
利用二次谐波产生显微镜评估扁桃体组织中成熟和不成熟的胶原蛋白
Kay Polland,Megan Clapperton,Thushitha Kunanandam et al.
Kay Polland et al.
Recurrent tonsillitis (RT) and obstructive sleep apnoea (OSA) are major indications for tonsillectomy, yet their tissue-level pathology remains unclear. Recurrent inflammation in RT may induce collagen remodelling through excess extracellul...
Mar García-Ferrés,Amy L Bottomley,Louise Cole et al.
Mar García-Ferrés et al.
Global BioImaging (GBI) and its regional and national partners, such as Canada BioImaging (CBI), BioImaging North America (BINA), Latin America BioImaging (LABI), the African BioImaging Consortium (ABIC) and Microscopy Australia (MA), have ...
The changing landscape of training in microscopy facilities: Reflections on seven decades of research user support at the University of Sydney [0.03%]
显微设施培训格局的变化：悉尼大学七十年研究支持的回顾
Eleanor Kable,Matthew Foley,Vijay Bhatia et al.
Eleanor Kable et al.
This thematic contribution highlights the critical role of established professional training regimes in microscopy and microanalysis in supporting high-quality research. As microscopy technologies continue to evolve in complexity, the risk ...