Background removal in fluorescence microscopy images based on baseline estimation [0.03%]
基于基线估计的荧光显微图像背景去除方法
Zhengyu He,Shang Zeng,Heguo Peng et al.
Zhengyu He et al.
Wide-field fluorescence microscopy is fundamentally limited by out-of-focus background haze, which degrades image contrast and obscures subcellular details. To overcome these limitations without the complexity of specialised hardware, we pr...
Clodomiro Cafolla,Marcello Campione
Clodomiro Cafolla
Friction plays a crucial role in both natural phenomena, from the flow of blood cells to earthquakes, and technological applications, from car engines to wind turbines. One of the most fundamental aspects of tribology is friction anisotropy...
Alfonso J Schmidt,Ellie Cho,Nicholas D Condon et al.
Alfonso J Schmidt et al.
Microscopy core facilities are critical to modern research, providing access to advanced microscopy applications and expert support. Despite this, global benchmarking data on facility operations, staffing and sustainability remain limited. ...
Silvia Melina Velasquez,Georgina Fletcher,Jean-Marie Burel et al.
Silvia Melina Velasquez et al.
Effective management of imaging data is critical to ensuring reproducibility, accessibility, and long-term value of image-based biological research. However, good data management remains a major challenge across imaging facilities, with lac...
CryoFluorSEM - A new approach for fluorescence and EM imaging of cryofractured plant samples [0.03%]
用于冷冻断裂植物样本的荧光和电子显微镜成像的新方法CryoFluorSEM
Raymond Wightman,Gareth Evans,Aram Gurzadyan et al.
Raymond Wightman et al.
Cryo-scanning electron microscopy (CryoSEM) permits the preparation and detailed imaging of bulky samples while keeping them in a hydrated state. For plant biology, cryofractures give information on cell ultrastructure and tissue organisati...
Reduced order modelling of air-puff test for corneal material characterisation [0.03%]
简化空气弹试验模型以表征角膜组织材料属性
Osama M Maklad,Muting Hao
Osama M Maklad
Models of the fluid-structure interaction (FSI) model for the air-puff test were analysed. Using Abaqus, the air-puff test is applied to eyes with varying biomechanical parameters, such as material properties, corneal thickness, and radius....
Local spatial distribution of J-aggregates in molecularly oriented bisazo-dye thin films [0.03%]
分子取向的重氮染料薄膜中的J聚集体的局域空间分布
Jian Yu,Tetsuya Aoyama,Atsuya Muranaka et al.
Jian Yu et al.
The spatial distribution of partially grown J-aggregates in films of oriented linear bisazo-dye molecules is analysed using polarised optical microscopy (POM), atomic force microscopy (AFM), and hyperspectral imaging. By depositing the bisa...
From projection to true surface: Curvature-corrected SEM morphometrics of diatom frustules under hydration variability [0.03%]
从投影到真实表面：hydration变化下的曲率修正SEM形态计量学应用于硅藻壳体
Izabela Zglobicka,Maciej Łojkowski,Ewa Borucinska et al.
Izabela Zglobicka et al.
Diatom frustules exhibit highly organised silica architectures with submicron features, which are widely studied. Nowadays, scanning electron microscopy (SEM) is commonly used for studying their morphology. However, SEM provides a two-dimen...
A Bayesian approach to spectroscopic depth sectioning for locating dopant atoms [0.03%]
用于定位受主原子的光谱深度分量的贝叶斯方法
Michael Deimetry,Timothy C Petersen,Matthew Weyland et al.
Michael Deimetry et al.
Locating dopants in 3D is of great interest as advanced materials and devices increasingly rely on control at atomic dimensions, and microscopy tools are constantly in development for this purpose. One potential tool is electron energy loss...