Remotely accessible optical microscopy education through a wide (and global) lens [0.03%]
远程访问的光学显微镜教育——透过广泛(全球性)的角度看问题
Zachary Sanchez,Miguel de Jesus,Moses David et al.
Zachary Sanchez et al.
Access to rigorous optical microscopy education remains unevenly distributed across the globe despite widespread use of optical methods. While lower-resourced settings certainly feel this burden, it is far from a foregone conclusion that su...
RNA-based CLEM (RCLEM) bridging RNA localisation and ultrastructural mapping in 3D [0.03%]
基于RNA的CLEM（RCLEM）在3D中连接RNA定位和超结构映射
Daan Verhaege,Lore Van Acker,Camille Wagner et al.
Daan Verhaege et al.
Spatial transcriptomics and in situ hybridisation techniques have become essential for contextualising single-cell RNA sequencing (scRNA-seq) data by mapping cell type- and state-specific mRNA expression within intact tissues. Beyond valida...
BIOMERO 2.0: End-to-end FAIR infrastructure for bioimaging data import, analysis, and provenance [0.03%]
BIOMERO 2.0：生物影像数据导入、分析和原点的端到端FAIR基础设施
Torec T Luik,Joost de Folter,Rodrigo Rosas-Bertolini et al.
Torec T Luik et al.
We present BIOMERO 2.0, a major evolution of the BIOMERO framework that transforms OMERO into a FAIR-compliant (findable, accessible, interoperable, and reusable), provenance-aware bioimaging platform. BIOMERO 2.0 integrates data import, pr...
From EM to AI: Multiscale imaging of the secretory pathway with insights from plant cells [0.03%]
从电子显微镜到人工智能：借助植物细胞的多尺度分泌途径成像及新见解
Charlotte Pain,Emily Farley
Charlotte Pain
The secretory pathway is a central and evolutionarily conserved feature of eukaryotic cells, responsible for protein and lipid trafficking, membrane biogenesis, signalling, and cellular homeostasis. Its complexity, dynamic behaviour, and na...
Mohammad Goudarzi,Maximilian Schuster,Arthur Milberger et al.
Mohammad Goudarzi et al.
Three-dimensional (3D) printing has rapidly developed from a niche hobbyist activity into a widely accessible and indispensable technology across multiple scientific disciplines. Within microscopy, optical engineering laboratories and imagi...
Kirk J Czymmek,Alexander Scott,Jessica B Back et al.
Kirk J Czymmek et al.
As part of two open international core facility surveys focused on microscopy and flow cytometry, we obtained an in-depth snapshot of their state-of-play. Here we focus on microscopy cores across 'the Americas', 'Asia Pacific' and 'Europe a...
Alison J North,Kurt I Anderson
Alison J North
Scientific reproducibility is an ethical and societal responsibility. Discussions of reproducibility and repeatability often focus on the roles of stakeholders in the scientific process, including researchers, publishers, and funders. Core ...
Hyperspectral tomographic diffractive microscopy: Development and applications [0.03%]
超光谱层析衍射显微镜：发展与应用
Leonardo Pestana Legori,Carlos Alberto Chacón Ávila,Matthieu Debailleul et al.
Leonardo Pestana Legori et al.
Tomographic Diffractive Microscopy (TDM) provides label-free three-dimensional imaging of transparent samples with resolution surpassing confocal limits. At IRIMAS, successive instrumental developments since 2009 have enhanced TDM capabilit...