Defocus correction and noise reduction using a hybrid ptychography and Centre-of-Mass algorithm [0.03%]
基于混合全息成像和质心算法的离焦矫正及去噪方法
Zhiyuan Ding,Chen Huang,Adrián Pedrazo-Tardajos et al.
Zhiyuan Ding et al.
Integrated Centre-of-Mass (iCOM) is a widely used phase-contrast imaging method based on Centre-of-Mass (COM), which makes use of a 4D Scanning Transmission Electron Microscopy (STEM) dataset using an in-focus probe. In this paper, we intro...
John Rodenburg
John Rodenburg
Application of STEM tomography to investigate smooth ER morphology under stress conditions [0.03%]
应用STEM断层扫描研究应激条件下的光滑内质网形态
V Heinz,R Rachel,C Ziegler
V Heinz
The endoplasmic reticulum (ER) is a highly dynamic organelle that undergoes significant morphological alterations in response to cellular stress. While conventional transmission electron microscopy (TEM) has provided valuable insights into ...
Clarissa Read,Ulla Neumann
Clarissa Read
Editorial
Journal of microscopy. 2025 Aug 11. DOI:10.1111/jmi.70023 2025
PRIAMOS: A technique for mixing embedding media for freely adjusting pH value and refractive index (RI) for optical clearing (OC) of whole tissue samples [0.03%]
PRIAMOS：一种混合嵌入介质的技术以自由调节pH值和折射率（RI）对整个组织样本进行光学清除（OC）
Ulrich Leischner,Martin Reifarth,Monika S Brill et al.
Ulrich Leischner et al.
Investigations of biological samples often require sample transparency, which is achieved by embedding the sample in a high-refractive index (RI) medium to obtain a homogenous RI distribution in the sample, referred to as optical clearing (...
A quantitative irradiation microscopy platform for in situ observation of living cells with subcellular resolution under radiation conditions [0.03%]
一种定量辐照显微镜平台 可在辐射条件下对活细胞进行亚细胞分辨率的原位观察
Zhao Chen,Liang Li,Jianli Liu et al.
Zhao Chen et al.
To address the critical need for investigating proton radiation effects on living cells in space environments and deciphering biological mechanisms underlying low-dose cumulative radiation effects, this study developed a microbeam irradiati...
Dawei Gao,Yu Yuan,Nicolas Brodusch et al.
Dawei Gao et al.
This manuscript presents a comparative analysis of two software packages, MC X-ray and PENELOPE, focusing on their accuracy and efficiency in simulating k-ratios for binary compounds and comparing their spectra with experimental data for pu...
A novel experimental platform to monitor solid/water interfaces under freeze-thaw cycles [0.03%]
一种监测固/水界面冻融过程的新实验平台
Chiara Recalcati,Rossella Yivlialin,Lamberto Duò et al.
Chiara Recalcati et al.
We design and implement an original experimental platform resting on Atomic Force Microscopy (AFM) to capture nanoscale insights into key characteristics of solid/water interfaces subject to freeze-thaw conditions. The work is motivated by ...
Ultrastructural expansion microscopy reveals unexpected levels of glycosome heterogeneity in African trypanosomes [0.03%]
超结构膨胀显微镜揭示非洲锥虫糖体异质性的新水平
Heidi Anderson,Rhonda Reigers Powell,Meredith Teilhet Morris
Heidi Anderson
Kinetoplastid parasites include several species. Trypanosoma brucei causes African sleeping sickness in humans and a wasting disease nagana in livestock. Trypanosoma cruzi is the causative agent of Chagas disease and Leishmania species caus...
Analytical solution of the classical Rayleigh length definition, including truncation at arbitrary values [0.03%]
经典Rayleigh长度定义的分析解，包括任意值截断的情况
Aufried Lenferink,Cees Otto
Aufried Lenferink
We present the analytical solution to the diffraction integral that describes the Rayleigh length for a focused Gaussian beam with any value of a spherical truncating aperture. This exact solution is in precise agreement with numerical calc...