Optical properties of cicada wings covered by graphene studied by nano-Raman spectroscopy [0.03%]
基于纳米拉曼光谱的石墨烯修饰蝉翅光学性质研究
Vitor A F Torres,André Pereira,Diego Sier et al.
Vitor A F Torres et al.
Some biological systems exhibit nanoscale constructions to produce optical effects. This study utilises Atomic Force Microscopy (AFM) and Tip-Enhanced Raman Spectroscopy (TERS) to study the complex bionanometric structure of cicada wings. T...
Revisiting PSF models: Unifying framework and high-performance implementation [0.03%]
重新审视点扩散函数模型：统一框架和高性能实现方法
Yan Liu,Vasiliki Stergiopoulou,Jonathan Chuah et al.
Yan Liu et al.
Localisation microscopy often relies on detailed models of point-spread functions. For applications such as deconvolution or PSF engineering, accurate models for light propagation in imaging systems with a high numerical aperture are requir...
A detailed protocol for expansion microscopy of Paracentrotus lividus embryos and larvae: Incorporating decalcification for improved imaging [0.03%]
一只海胆胚胎和幼体的详细膨胀显微镜协议：改进成像的脱钙作用
Thomas M D Sheard,Davide Sartori,Matteo Colombo et al.
Thomas M D Sheard et al.
Expansion microscopy (ExM) enables superresolution imaging by embedding biological specimens in a swellable hydrogel, followed by optical clearing and physical expansion. Here we present a detailed protocol for applying ExM to embryonic sta...
Reza Yazdi,Hassan Khotanlou
Reza Yazdi
Accurate detection of mitosis is crucial in automated cell analysis, yet many existing methods depend heavily on deep learning models or complex detection techniques, which can be computationally intensive and error-prone, particularly when...
Andrew Maiden,Laura Clark,Peter O&#x;Toole
Andrew Maiden
Depth of field of multi-slice electron ptychography: Investigating energy and convergence angle [0.03%]
多切片电子ptycho图形的景深：研究能量和收敛角的影响
Frederick Allars,Andrew Maiden,Darren J Batey et al.
Frederick Allars et al.
Multi-slice electron ptychography has attracted significant interest in recent years, thanks to notable experimental successes in ultra-high resolution, depth-resolved imaging of atomic structure. However, the theoretical dependence of dept...
Surface visualisation of bacterial biofilms using neutral atom microscopy [0.03%]
中性原子显微镜在细菌生物被膜表面观测中的应用
Nick A von Jeinsen,David J Ward,Matthew Bergin et al.
Nick A von Jeinsen et al.
The scanning helium microscope (SHeM) is a new technology that uses a beam of neutral helium atoms to image surfaces non-destructively and with extreme surface sensitivity. Here, we present the application of the SHeM to image bacterial bio...
A fluorescence lifetime separation approach for FLIM live-cell imaging [0.03%]
一种用于FLIM活细胞成像的荧光寿命分离方法
Cornelia Wetzker,Marcelo Leomil Zoccoler,Svetlana Iarovenko et al.
Cornelia Wetzker et al.
Fluorescence lifetime imaging microscopy (FLIM) translates the duration of excited states of fluorophores into lifetime information as an additional source of contrast in images of biological samples. This offers the possibility to separate...
Correlation of near-field optical microscopy and tip-assisted photoluminescence [0.03%]
近场光学显微镜与针尖增强光致发光的关联性研究
W Pfeiffer,N S Mueller,R Hillenbrand et al.
W Pfeiffer et al.
Nanoscale optical imaging has unlocked unprecedented opportunities for exploring the structural, electronic, and optical properties of low-dimensional materials with spatial resolutions far beyond the diffraction limit. Techniques such as t...
Microstructural characterisation of polycrystalline ice with an etch-pitting replication method [0.03%]
利用腐蚀凹坑复制法表征多晶冰的显微组织结构特征
Hatsuki Yamauchi,Lucy Davidson,Christine McCarthy et al.
Hatsuki Yamauchi et al.
Etch-pitting replication is a classical method to characterise the microstructure of ice crystals. In this method, a solution of polyvinyl formal (Formvar) is applied to a polished surface of ice. A plastic film, created after the solvent i...