HIST-DIP: histogram thresholding and deep image priors assisted smartphone-based fluorescence microscopy imaging [0.03%]
基于直方图阈值和深度图像先验的智能手机荧光显微镜成像技术
Harshitha Govindaraju,Muhammad Nabeel Tahir,Umer Hassan
Harshitha Govindaraju
Portable fluorescence microscopes coupled with smartphones offer accessible and cost-effective point-of-care diagnostic solutions, but often produce noisy and blurry images with poor contrast. Here, we introduce HIST-DIP (HIStogram Threshol...
A colloidal gold immunochromatographic assay for on-site lead detection in vegetable oil [0.03%]
一种现场检测植物油中铅的胶体金免疫层析方法
Jiaxun Li,Liuping Zhang,Guoping Qian et al.
Jiaxun Li et al.
Lead (Pb) contamination in vegetable oil poses a significant threat to human health, and traditional detection methods have many limitations. In this study, a monoclonal antibody (mAb) against Pb2+ was prepared, and a colloidal gold immunoc...
PAM-free activation of CRISPR/Cas12a via semi-nested asymmetric RPA: highly specific detection of HPV16 dsDNA [0.03%]
基于半嵌合不对称重组酶聚合酶放大技术的Cas12a检测HPV16 DNA
Xiaozhi Zou,Tao Gu,Xuheng Li et al.
Xiaozhi Zou et al.
Early and accurate detection of HPV16 nucleic acids is therefore critical for the effective screening, diagnosis, and prevention of cervical cancer. Although CRISPR/Cas12a-based molecular diagnostics offer a rapid and sensitive approach for...
Qianying Xu,Hadeer Mattar,Emmanuelle Claude et al.
Qianying Xu et al.
Scope: The Caco2/HT29-MTX co-culture system is widely used as a cell model of the intestinal epithelium. Although the gut epithelium plays an important role in the uptake of free fatty acids and the resynthesis of triglycerides, the lipid d...
Secretion analysis during the invasion process of Salmonella typhimurium into HT-29 intestinal epithelial cells on a microfluidic chip [0.03%]
一种用于分析鼠伤寒沙门氏菌入侵HT-29肠上皮细胞过程中分泌情况的微流控芯片及其应用方法
Hong He,Haolan Tan,Chuang Ge et al.
Hong He et al.
In this study, a contact co-culture model of bacteria and cells on a microfluidic chip with arrayed circular chambers was established to simulate the invasion process of Salmonella typhimurium (S. typhimurium) into HT-29 intestinal epitheli...
Advanced Raman assisted metabolic labelling: an impending approach for exploring metabolites in cellular biosynthetic processes [0.03%]
先进的拉曼辅助代谢标记法：探索细胞生物合成过程中代谢物的即将来临的方法
Madhukrishnan Murali,Kaustabh Kumar Maiti
Madhukrishnan Murali
Metabolic labelling has become essential in revealing the complex characteristics of metabolite activity and the metabolic processes within cells. When coupled with non-destructive, ultra-sensitive Raman spectroscopy, it enables the visuali...
Review
The Analyst. 2025 Jul 25. DOI:10.1039/d5an00586h 2025
X-ray induced modifications in U87 glioma cells probed by Raman- and infrared-based spectromicroscopy [0.03%]
基于拉曼和红外光谱的显微技术研究X射线照射引起的U87胶质瘤细胞改变
T Senapati,M R Bittermann,R Nadar et al.
T Senapati et al.
A combination of spontaneous Raman, stimulated Raman, and photothermal expansion (AFM-IR) spectromicroscopy is reported for probing the impact of different radiation doses (2-10 Gy) on U87 glioma cells ex vivo. Most significant are alterati...
ESIPT-active fluorescent probes for ratiometric analysis of carbonate ions in aqueous media: structural modifications for enhanced selectivity and response [0.03%]
用于水相中碳酸根离子比色分析的ESIPT型荧光探针：结构改造增强选择性和响应性
M Gayathri,Sourav Mondal,D Sriram et al.
M Gayathri et al.
This study focuses on the design and synthesis of two ESIPT-active fluorescent probes that differ in the position of the hydroxyl group. The isomer with the hydroxyl group at the ortho position in compound 1 exhibited a large population of ...
Development of a light-initiated chemiluminescence system for the detection of human plasma p-tau181 [0.03%]
开发用于检测人类血浆p- tau181的光引发化学发光系统
Yang Yang,Xu Wang
Yang Yang
Phosphorylated tau 181 (p-tau181) is a promising biomarker for predicting and monitoring Alzheimer's disease (AD) neuropathology. However, existing commercial assays suffer from disadvantages such as high cost and insufficient detection sen...
A holistic approach to understanding biochemical degradation of human tissues using high resolution MALDI MS [0.03%]
基于高分辨MALDI质谱的人体组织生化降解的全面分析方法研究
Jerika Ho,Naomi L Stock,Vaughn Mangal et al.
Jerika Ho et al.
Estimating the postmortem interval (PMI) is crucial in medico-legal death investigations, but existing methods are highly influenced by environmental and physiological factors. This study proposes a novel biochemical strategy for PMI estima...