Casey J Toft,Holly M Radford,Alanna E Sorenson et al.
Casey J Toft et al.
The characterization of protein-DNA interactions underpinning fundamental biological processes requires methods that are laborious and time-consuming. Here, we introduce a rapid and instrument-free assay leveraging on GFP-tagged proteins an...
Jakub Zastąpiło,Robyn A Emmerson,Liudmila A Mikheeva et al.
Jakub Zastąpiło et al.
Non-genetic variability in gene expression is an inevitable consequence of the stochastic nature of processes driving transcription and translation. While previous studies demonstrated that gene expression noise is negatively correlated wit...
Transposable elements as drivers of genome evolution in Drosophila virilis [0.03%]
Drosophila virilis基因组进化中的可移动元件
Alexander P Rezvykh,Dina A Kulikova,Elena S Zelentsova et al.
Alexander P Rezvykh et al.
Transposable elements (TEs) drive genomic innovation, but their dynamics in non-model species remain unclear. Here, we integrated multi-omics data to explore TE dynamics in Drosophila virilis, an important model for repetitive DNA research....
DNA polymerase λ autoinhibition is relieved via Ku interaction during non-homologous end joining [0.03%]
Ku介导的DNA聚合酶λ去抑制在非同源末端连接中的作用
Brandon C Case,Leonardo Scoccia,Zhihan Zhao et al.
Brandon C Case et al.
DNA ends are frequently damaged during the formation of DNA double-strand breaks (DSBs). These ends must be repaired to enable ligation during non-homologous end joining (NHEJ). NHEJ uses several end processing factors to repair DNA ends wi...
Improved reconstruction of transcripts and coding sequences from RNA-seq data [0.03%]
改进RNA测序数据的转录本和编码序列重建方法
Jan Grau,Deborah Weise,Marika Panster et al.
Jan Grau et al.
Annotation of genes and transcripts is a key prerequisite for understanding the information that is encoded in newly sequenced genomes. One source of information suited for this purpose is RNA-seq data mapped to the respective genome sequen...
Energetically equivalent structural transitions in the Rad17-Rad9-Hus1-Rad1-Rhino complex underlie the sequential progression from activation through maintenance to inactivation of the ATR-dependent DNA damage response [0.03%]
Rad17-Rad9-Hus1-Rad1-Rhino复合物中的能量等效结构转换调控ATR依赖的DNA损伤反应从激活到维持再到失活的顺序进程
Yasunori Fukumoto,Ryuzaburo Yuki,Yasumitsu Ogra
Yasunori Fukumoto
Activation of the ATR-dependent DNA damage response (ATR-DDR) is well characterized; however, the molecular mechanisms underlying its maintenance and inactivation remain largely elusive. Rhino is the least understood component of ATR-DDR. S...
Toehold-VISTA: a machine learning approach to decipher programmable RNA sensor-target interactions [0.03%]
基于机器学习的解析可编程RNA传感器靶点相互作用的方法
James M Robson,Alexander A Green
James M Robson
RNA-based biosensors have emerged as essential tools in synthetic biology and diagnostics, enabling precise and programmable responses to diverse RNA inputs. However, the time to design, produce, and screen high-performance RNA sensors rema...
Yan Xia,Jinyuan Sun,Xiaowen Du et al.
Yan Xia et al.
Deep learning has successfully been applied to design cis-regulatory elements (CREs) for a few species, but a broadly applicable platform for generating functional promoters for thousands of prokaryotes remains lacking. In this study, we in...
Complex cooperativity in DNA origami revealed via design-dependent defectivity [0.03%]
基于设计缺陷性揭示DNA折纸物中的复杂协同作用
Jacob M Majikes,Amna Hasni,Shankar Haridas et al.
Jacob M Majikes et al.
DNA origami has become a ubiquitous platform because it enables straightforward design of nanostructures that self-assemble with high yield. The interactions between the cooperative effects involved in its assembly are currently not well un...
Engineering a human-based translational activator for targeted protein expression restoration [0.03%]
工程化的人源性转录激活剂以实现蛋白质表达的靶向恢复
Riley W Sinnott,Ani Solanki,Anitha P Govind et al.
Riley W Sinnott et al.
Therapeutic modalities to programmably increase protein production are in critical need to address diseases caused by deficient gene expression via haploinsufficiency. Restoring physiological protein levels by increasing translation of thei...