The tumor suppressor menin is a key scaffold mediator for homologous recombination repair [0.03%]
肿瘤抑制因子menin是同源重组修复的关键支架介质
Aobo Lian,Xingwen Zhu,Bangming Jin et al.
Aobo Lian et al.
SETD2-mediated H3K36me3 reprogramming is involved in homologous recombination (HR) repair; however, the dynamics of this repair process remain unclear. Here, we report that the scaffold protein menin, encoded by the Men1 gene, plays a cruci...
Duplication of specialized cis-acting elements SLI and SLIII in the viral RNA 3'UTR enables mosquito-borne orthoflavivirus adaptation to avian hosts [0.03%]
病毒RNA的3' UTR中特化顺式作用元件SLI和SLIII的重复使蚊媒黄病毒能够适应鸟类宿主
Li Mao,Yu He,Tao Wang et al.
Li Mao et al.
Cross-species transmission of mosquito-borne orthoflaviviruses (MBFVs) relies on multi-host switching and adaptation, yet the underlying mechanisms remain incompletely understood. Domain I of the 3'-untranslated region (UTR) is highly struc...
Structural characterization of ribosome recycling and fusidic acid inhibition in Staphylococcus aureus [0.03%]
金黄色葡萄球菌核糖体解离和满山红酸抑制的结构表征
Adrián González-López,Maria Selmer
Adrián González-López
During bacterial ribosome recycling, 70S ribosomes are split into subunits by ribosome recycling factor (RRF) and elongation factor G (EF-G). The antibiotic fusidic acid (FA) inhibits elongation and ribosome recycling by locking EF-G to the...
Nucleostemin promotes RAD51 filament assembly on double-stranded DNA to protect stalled replication forks [0.03%]
核仁干细胞因子通过促进RAD51在双链DNA上形成丝状结构来保护受阻的复制叉
Chih-Chun Chang,Siang-Sheng Tsai,Chang-Le Chou et al.
Chih-Chun Chang et al.
Replication stress threatens genome integrity by stalling replication forks, which may lead to double-stranded DNA (dsDNA) breaks. Stalled replication forks can be rescued through the fork reversal mechanism, yet they remain vulnerable to n...
Genome-wide mapping of replication stress response factors reveals the management of polycistronic transcription, replication initiation, and responses to replication stress in Leishmania [0.03%]
全基因组复制压力反应因子定位揭示莱什曼原虫中多顺反子转录、复制起始和复制压力反应的调控机制
Jennifer A Black,Stela Virgilio,Matheus S Bastos et al.
Jennifer A Black et al.
Exposed single-stranded DNA (ssDNA) accumulating at stalled or collapsed replication forks (RFs) can trigger the replication stress response (RSR) to prevent genome instability. Leishmania are parasitic eukaryotes whose plastic genomes faci...
Fast assembly and in vivo coalescence of ParBF biocondensates involved in bacterial DNA partition [0.03%]
快速装配并在活细胞中聚集参与细菌DNA分配的ParBF生物凝聚物
Perrine Revoil,Linda Delimi,Jérôme Rech et al.
Perrine Revoil et al.
Faithful DNA segregation in bacteria relies on ParABS systems, in which ParB assembles into condensates at centromere-like parS sites, while the ATPase ParA spatially organizes these complexes. How these ParB condensates maintain dynamic be...
Fast assembly and in vivo coalescence of ParBF biocondensates involved in bacterial DNA partition [0.03%]
快速组装并参与细菌DNA分配的ParBF生物凝聚体的体内融合
Perrine Revoil,Linda Delimi,Jérôme Rech et al.
Perrine Revoil et al.
Faithful DNA segregation in bacteria relies on ParABS systems, in which ParB assembles into condensates at centromere-like parS sites, while the ATPase ParA spatially organizes these complexes. How these ParB condensates maintain dynamic be...
A self-iterative orthogonal base-editing platform enables multiplex N-to-N diversification and genome-scale functional screening in Escherichia coli [0.03%]
一种自我迭代的正交碱基编辑平台能够在大肠杆菌中实现多重N到N多样化及全基因组规模的功能筛选
Xiangrui Fan,Liya Liang,Hongle Wang et al.
Xiangrui Fan et al.
Base editing enables precise genome modification without double-strand breaks but remains limited by narrow editing windows, DNA repair pathway biases, and restricted nucleotide diversity. Here, we report MUTATOR, a MUlTiplexAble and self-i...
A self-iterative orthogonal base-editing platform enables multiplex N-to-N diversification and genome-scale functional screening in Escherichia coli [0.03%]
一种自我迭代的正交碱基编辑平台能够使大肠杆菌中进行N到N的多路分型以及基因组范围的功能筛选
Xiangrui Fan,Liya Liang,Hongle Wang et al.
Xiangrui Fan et al.
Base editing enables precise genome modification without double-strand breaks but remains limited by narrow editing windows, DNA repair pathway biases, and restricted nucleotide diversity. Here, we report MUTATOR, a MUlTiplexAble and self-i...
The ZN domain acts as a dynamic switch coordinating multiple-step aminoacylation in bacterial leucyl-tRNA synthetase [0.03%]
ZN结构域作为动态开关协调细菌亮氨酰-tRNA合成酶的多步骤氨酰化作用
Guillaume Hoffmann,Morana Dulić,Ita Gruic-Sovulj et al.
Guillaume Hoffmann et al.
Aminoacyl-tRNA synthetases (AARSs) safeguard translational fidelity by coordinating amino acid activation and tRNA charging within distinct catalytic and editing domains. In leucyl-tRNA synthetase (LeuRS), the small, centrally located zinc-...