Shuttling of RecQ helicase HIM-6 coordinates iterative cycle of unwinding, pulling, and backsliding [0.03%]
HIM-6 RecQ解旋酶的穿梭作用协调了重复进行的解开、拉动和回拖循环过程
Son Truong Le,Seung-Won Lee,Chanwoo Kim et al.
Son Truong Le et al.
RecQ DNA helicases are a highly conserved family of proteins essential for maintaining genome stability. Despite smFRET studies on repetitive DNA unwinding by RecQ using fluorophore-labeled DNA substrates, the domains controlling this behav...
Orn-mediated c-di-GMP regulates the CRISPR-Cas system to confer stress response in Mycobacterium tuberculosis [0.03%]
由Orn介导的c-di-GMP通过调节CRISPR-Cas系统赋予结核分枝杆菌压力响应反应
Wenjing Yu,Lisha Yuan,Wei Zhou et al.
Wenjing Yu et al.
Mycobacterium tuberculosis (Mtb) possesses a type III-A CRISPR-Cas system and has anti-plasmid immune activity. However, whether this system exerts other additional functions remains to be characterized. Here, we investigated the in vivo ro...
In vitro reconstitution of heterochromatin compartments reveals the spontaneous formation of tunable liquid-liquid interfaces [0.03%]
体外重构异染色质隔室揭示了可调液-液界面的自发形成
Lucy D Brennan,Hyeong-Ku Kim,Serafin Colmenares et al.
Lucy D Brennan et al.
Compartmentalization of the genome into heterochromatin and euchromatin is a highly conserved and essential process across eukaryotes. Constitutive heterochromatin (C-Het) packages the repetitive regions of the genome within a biomolecular ...
The Lsm1-7 complex couples 3'-end protection to histone H4 acetylation to maintain mRNA homeostasis in Fusarium graminearum [0.03%]
Lsm1-7复合体通过将3'-端保护与组蛋白H4乙酰化相偶联来维持小麦赤霉病菌中的mRNA稳态
Yiyi Ren,Jiayue Yan,Xingmin Han et al.
Yiyi Ren et al.
Eukaryotic messenger RNA (mRNA) homeostasis requires precise coordination between synthesis and decay, yet the mechanisms governing this balance in fungal pathogens remain elusive. Here we provide a comprehensive characterization of the Lsm...
Alternative polyadenylation links RNA processing to iron metabolism in human erythropoiesis [0.03%]
人类红细胞生成中的替代聚腺苷酸化将RNA加工与铁代谢联系起来
Shan Yu,Xianyan Zeng,Jing Chen et al.
Shan Yu et al.
Erythropoiesis requires precise coordination of transcriptional and co-/post-transcriptional programs, yet the role of alternative polyadenylation (APA) in this process remains poorly understood. Here, we profiled the genome-wide dynamic AP...
Tamara Eleanore Hamann,Angela Wieland,Farbod Mohseni et al.
Tamara Eleanore Hamann et al.
The BLM helicase is a critical genome maintenance protein involved in diverse cellular processes including DNA replication, repair, transcription, and chromosome segregation. During mitosis, it cooperates with the PICH helicase and topoisom...
Cruciform-forming AT/TA repeats are acted upon by structure-selective endonucleases and Rad51 prior to repositioning to the nuclear periphery for repair [0.03%]
十字形结构的AT重复序列由结构特异性内切酶和Rad51处理，并在修复前移至核周区域
Jan Leendert Boer,Sara M Crippen,Amelia J Kim et al.
Jan Leendert Boer et al.
Structure-forming DNA repeats can pose a barrier to DNA replication and repair, creating chromosomal fragile sites. An AT/TA DNA repeat, derived from the Flex1 region of human common fragile site FRA16D, can form hairpin and cruciform struc...
La protein binding to telomerase RNA supports an evolutionary relationship between plant and ciliate telomerase pathways [0.03%]
LA蛋白与端粒酶RNA的结合支持了植物和纤毛动物端粒酶途径之间的进化关系
Leon Jenner,Dzmitry Pruchkouski,Barbora Štefanovie et al.
Leon Jenner et al.
The Arabidopsis thaliana La1 (AtLa1) protein is a member of the genuine La family of RNA biogenesis proteins, which are structurally similar to the La-related protein 7 (LARP7) family. LARP7 proteins participate in the biogenesis of the tel...
A spring-loaded grip-and-pull mechanism for stepwise RNA duplex unwinding by Xrn1 [0.03%]
Xrn1通过弹簧加载的夹持和拉伸机制分步解开RNA二聚体
Junhyuk Rhee,Hyeokjin Cho,Semi Hong et al.
Junhyuk Rhee et al.
Xrn1 is a highly conserved 5'→3' exoribonuclease that plays a central role in RNA turnover and quality control in eukaryotic cells. Although Xrn1 is known to degrade single-stranded RNA in a processive manner, the mechanism by which it eng...
Repeat-rich RNA guides repetitive genomic elements into biomolecular condensates for heterochromatin organization and muscle integrity [0.03%]
富含重复序列的RNA通过引导基因组重复元件进入生物分子凝聚物来组织异染色质并维持肌肉完整性
Jinmi Choi,Sangha Park,Nahee Kim et al.
Jinmi Choi et al.
Biomolecular condensation is a pivotal mechanism in chromatin organization and nuclear compartmentalization. However, the molecular mechanism that drives heterochromatin organization and selectively partitions heterochromatin components in ...