Characterization of gRNA-dependent and gRNA-independent off-target binding sites of PspCas13b and RfxCas13d in mammalian cells [0.03%]
PspCas13b和RfxCas13d在哺乳动物细胞中gRNA依赖性和非依赖性脱靶结合位点的表征
Huanhuan Feng,Zhixue Li,Hongdao Zhang et al.
Huanhuan Feng et al.
CRISPR-Cas13 systems, harnessed for RNA-guided transcriptome editing, hold significant promise for clinical and in vivo therapeutic applications. However, understanding their in vivo target specificity and recognition rules remains a challe...
PolyP inhibits CobB deacetylase triggering a regulatory coupling to prevent DNA replication initiation [0.03%]
聚磷酸盐通过抑制CobB脱乙酰酶触发调控耦合以防止DNA复制启动
Ewelina Boguszewska,Zuzanna Hirsz,Magdalena Sroka et al.
Ewelina Boguszewska et al.
Polyphosphate (polyP) is considered having regulatory functions in both procaryotic and eucaryotic cells. Under certain stress conditions, bacteria accumulate polyP, which results in liquid-liquid phase separation and polyP granules formati...
Isoform-specific single-cell perturb-seq reveals distinct functions of alternative promoters in drug response [0.03%]
同种型特异性单细胞扰动测序揭示了替代启动子在药物反应中的不同功能
Helen E King,Savannah O&#x;Connell,Daisy Kavanagh et al.
Helen E King et al.
CRISPR interference (CRISPRi) screens have emerged as powerful tools for dissecting gene function, yet their application to genes with multiple promoters, which comprise over 60% of human genes, remains poorly understood. Here, we demonstra...
HCR-Proxy resolves site-specific proximal RNA microenvironments at subcompartmental resolution [0.03%]
利用HCR-Proxy在亚区室分辨率下解析位点特异性邻近RNA微环境
Anja Trupej,Valter Bergant,Jona Novljan et al.
Anja Trupej et al.
The spatial organization of RNA-scaffolded condensates is fundamental for understanding of basic cellular functions, but may also provide pivotal insights into diseases. One of the major challenges to understanding the role of condensates i...
Bromodomain protein IBD1 bridges histone acetylation and H2A.Z deposition to fine-tune transcription [0.03%]
溴结构域蛋白IBD1通过连接组蛋白乙酰化和H2A.Z沉积来精细调节转录
Zhe Zhang,Haicheng Li,Aili Ju et al.
Zhe Zhang et al.
Eukaryotic gene expression is dynamically regulated through the interplay between histone modifications and chromatin remodeling, yet how these processes are coordinated remains incompletely understood. Here, we uncover IBD1 as a critical a...
DDX24 modulates angiogenesis by promoting CCR4-NOT complex-dependent mRNA decay [0.03%]
DDX24通过促进CCR4-NOT复合物依赖的mRNA降解来调节血管生成
Simeng He,Bin Li,Fangbin Chen et al.
Simeng He et al.
DEAD-box (DDX) RNA helicases play critical roles in gene regulation by interacting with RNAs and influencing RNA fate and function. Our previous study associated DDX24 dysfunction with vascular development, but its precise role in RNA metab...
CRISPR-associated transposon for programmable viral vector engineering and prime editing [0.03%]
用于程序病毒载体工程和prime编辑的 crispr 相关转座子
Quyen T Dang,Chin-Wei Chang,Pin-Yan Chen et al.
Quyen T Dang et al.
Baculovirus, an insect virus commonly used for recombinant protein expression in insect cells and gene delivery in mammalian systems, is often generated through bacmid-based engineering. To enable flexible and programmable bacmid engineerin...
Xeno-nucleic acids support formation of Ag(I)-mediated duplexes and silver nanoclusters [0.03%]
外源核糖核酸支持形成Ag（I）介导的双链和银纳米团簇
Rweetuparna Guha,Eshana Bethur,Nicholas Chim et al.
Rweetuparna Guha et al.
The expanded backbone chemistries of xeno-nucleic acids (XNAs) hold significant promise for emerging areas of synthetic biology and nanomaterials, but metal-mediated XNA interactions remain largely unexplored. Here, we use a combination of ...
Takayuki Katoh,Hiroaki Suga
Takayuki Katoh
Sense codon reassignment enables ribosomal incorporation of nonproteinogenic amino acids (npAAs) at any of the 61 sense codons. Because npAAs replace proteinogenic amino acids (pAAs), the total number of available building blocks usually re...
TORCphysics: a physical model of DNA-topology-controlled gene expression [0.03%]
TORCphysics：DNA拓扑控制基因表达的物理模型
Victor Velasco-Berrelleza,Penn Faulkner Rainford,Aalap Mogre et al.
Victor Velasco-Berrelleza et al.
DNA superhelicity and transcription are intimately related because changes to DNA topology can influence gene expression and vice versa. Information is transferred through the modulation of local DNA torsional stress, where the expression o...