Metabolic isotopic labeling of (deoxy)ribose enables dual neutral loss scanning for profiling nucleic acid modifications [0.03%]
代谢同位素标记的(脱氧)核糖可实现双重中性丢失扫描以表征核酸修饰
Shao-Qin Rong,Zhi-Han Jin,Dong-Rui Yin et al.
Shao-Qin Rong et al.
Modifications on DNA and RNA, even at trace levels, play critical roles in diverse biological processes, yet their accurate quantification and discovery of novel modified constituents remain challenging. Here, we present a metabolic (deoxy)...
Correction to 'Precise homology-directed installation of large genomic edits in human cells with cleaving and nicking high-specificity Cas9 variants' [0.03%]
对「利用切割和切口型高特异性Cas9变异体在人类细胞中精确诊断大型基因组编辑的精确性」一文进行更正
Published Erratum
Nucleic acids research. 2026 Feb 5;54(4):gkag158. DOI:10.1093/nar/gkag158 2026
Ongoing structural changes highlight the dynamic nature of chloroplast genomes [0.03%]
正在进行的结构变化突显了叶绿体基因组的动态性质
Eranga Pawani Witharana,Nobuhiro Kotoda,Kiyohiko Seki et al.
Eranga Pawani Witharana et al.
High-throughput sequencing has transformed chloroplast genome research by enabling cost-effective analyses from shallow whole-genome sequencing datasets generated using short-read platforms, yet standardized approaches often overlook hidden...
Kęstutis Timinskas,Darius Kazlauskas,Albertas Timinskas et al.
Kęstutis Timinskas et al.
DNA polymerases are key players in DNA replication, repair, and maintenance. However, the overall abundance, diversity, and distribution of bacterial DNA polymerases have not been systematically explored. To close this knowledge gap, we com...
CFP1-mediated H3K4me3 broad domains control early B cell lineage fate determination [0.03%]
CFP1介导的H3K4me3广域调控早期B细胞谱系命运决定
Wanyu Bai,Yalin Yang,Yanan Zhao et al.
Wanyu Bai et al.
The histone H3K4me3 broad domain (BD) is a unique epigenetic feature marking cell identity-associated genes, but its physiological role in lineage differentiation remains unclear. Here we show that CFP1, an integral component of the Setd1A/...
Sabab Hasan Khan,Neela Yennawar,C Denise Okafor
Sabab Hasan Khan
The farnesoid X receptor (FXR) is a ligand-regulated transcription factor that primarily functions as an obligate heterodimer with retinoid X receptor alpha (RXRα). While evidence suggests that FXR can also bind DNA independently, the natu...
Genome-wide dynamic nascent transcript profiles reveal that most paused RNA polymerases terminate [0.03%]
全基因组新生动态转录本谱图表明大多数暂停的RNA聚合酶终止转录
Rudradeep Mukherjee,Michael J Guertin
Rudradeep Mukherjee
We present a simple model for analyzing and interpreting data from kinetic experiments that measure engaged RNA polymerase occupancy. The framework represents the densities of nascent transcripts within the pause region and the gene body as...
Cat_Wiz: a stereochemistry-guided toolkit for locating, diagnosing, and annotating Mg2+ ions in RNA structures [0.03%]
Cat_Wiz：一个立体化学导向的Mg2+离子定位、诊断及标注RNA结构工具包
Nawavi Naleem,Anja Henning-Knechtel,Serdal Kirmizialtin et al.
Nawavi Naleem et al.
Misassigned Mg2+ ions are pervasive in RNA structural databases, obscuring mechanistic interpretation, undermining comparative analyses, and compromising machine-learning training sets. Here, we present Cat_Wiz, a Coot-integrated, stereoche...