Minor spliceosome inhibition via ablation of its U11 snRNA gene results in multiple defects in murine spermatogenesis [0.03%]
通过消除其U11小核RNA基因抑制次要剪接体导致小鼠精子发生过程中出现多种缺陷
Kazumasa Takemoto,Kaitlin N Girardini,Abigail Boria et al.
Kazumasa Takemoto et al.
Minor intron-containing genes (MIGs), which require the minor spliceosome (MiS) for their splicing, are highly represented among genes involved in mouse spermatogenesis. Leveraging gene- and intron-level ortholog data, we show that conserva...
Mirko Weber,Felix Erichson,Maciej Antczak et al.
Mirko Weber et al.
Integrative biomolecular modeling of RNA relies on refined structural collections and accurate experimental data that reflect binding and folding behavior. However, predicting such collections remains challenging due to the rugged energy la...
hnRNPA2B1 induces HBV cccDNA degradation by recruiting APOBEC3B [0.03%]
hnRNPA2B1通过募集APOBEC3B诱导HBV cccDNA降解
Zhendong Fu,Liyuan Wang,Yang Sun et al.
Zhendong Fu et al.
Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) constitutes a viral persistence reservoir that sustains chronic infection. Although the DNA damage response (DDR) facilitates cccDNA biogenesis, its role in regulating cccDNA s...
SSB-mediated enhancement of argonaute activity triggers SOS filamentation in bacteria [0.03%]
SSB介导的Argonaute活性增强触发细菌SOS丝状生长反应
Yuan Shao,Pan Zhang,Mengyuan Su et al.
Yuan Shao et al.
Prokaryotic Argonaute proteins (pAgos) are emerging as versatile tools in nucleic acid processing; however, their roles in bacterial physiology remain poorly defined. Here, we demonstrate that overexpression of Thermus thermophilus Argonaut...
Spatially concentrated adenine base editors efficiently correct PLP1 mutations in oligodendrocytes [0.03%]
空间集中腺嘌呤碱基编辑器可有效纠正少突胶质细胞中的PLP1突变
Chi Zhang,Ke Ye,Yafang Shang et al.
Chi Zhang et al.
Oligodendrocytes (OLs), the myelinating cells of the central nervous system, are particularly prone to pathogenic G-to-A mutations, such as PLP1A243V, which causes Pelizaeus-Merzbacher disease (PMD), a lethal hypomyelinating disorder lackin...
Advancing codon language modeling with synonymous codon constrained masking [0.03%]
基于同义密码子约束掩码的密码子语言模型研究进展
James Heuschkel,Laura Kingsley,Noah Pefaur et al.
James Heuschkel et al.
Codon language models offer a promising framework for modeling protein-coding DNA sequences, yet current approaches often conflate codon usage with amino acid semantics, limiting their ability to capture DNA-level biology. We introduce SynC...
Luis F Montano-Gutierrez,Sophie Müller,Ana P Kutschat et al.
Luis F Montano-Gutierrez et al.
A prime goal of regenerative medicine is to replace dysfunctional cells in the body. To design protocols for producing target cells in the laboratory, one may need to consider exponentially large combinations of culture components. Here, we...
Olena Parilova,Piia Bartos,Anssi M Malinen
Olena Parilova
RNA polymerase I (Pol I) synthesizes precursor ribosomal RNA, a key step in ribosome biogenesis. Elevated Pol I activity supports rapid cell growth-a hallmark of cancer-making Pol I a therapeutic target. The initial step in synthesis involv...
Engineering of tRNAPro1E2 anticodon stem enhances multiple/consecutive ribosomal incorporation of N-methyl-l-α-amino acids and d-α-amino acids [0.03%]
工程化tRNAPro1E2反密码子茎可增强N-甲基-L-/D-α-氨基酸的多个/连续核糖体掺入
Ryoichi Hirashima,Takayuki Katoh,Hiroaki Suga
Ryoichi Hirashima
Transfer RNA (tRNA) structures influence the incorporation efficiency of amino acids, particularly when nonproteinogenic amino acids (npAAs), such as N-methyl-l-α-amino acids (MeAAs) and d-α-amino acids (d-AAs), are charged. Such npAAs ar...
NAD+ capping of sibD transcripts in E. coli is mediated by its minimal promoter and enhanced by ppGpp [0.03%]
Ecoli中sibD转录本的NAD+帽由其最小启动子介导并由ppGpp增强
Wuzhen Liu,Kefan Hu,Shiqi Nie et al.
Wuzhen Liu et al.
Recently, nicotinamide adenine dinucleotide (NAD+) and other nucleotide analogs have been identified as non-canonical RNA caps in both prokaryotes and eukaryotes. In Escherichia coli, NAD capping has been shown to be influenced by environme...