Mechanism of interaction between the transactivation domain of N-myc and the DNA-binding surface of TFIIIC5 [0.03%]
N-myc的反式激活域与TFIIIC5的DNA结合面相互作用的机制研究
Eoin Leen,Sharon Yeoh,Eka Sahak et al.
Eoin Leen et al.
N-myc is a transcription factor, a powerful driver of cellular growth and an important oncoprotein. N-myc interacts with many factors, including the RNA Polymerase III assembly factor, TFIIIC, a six-subunit complex that is essential for the...
Glycine max Jumonji C domain-containing proteins JMJ19/20 exhibit endopeptidase activity and interact with LUXs to mediate flowering time [0.03%]
大豆JMJ19/20基因通过介导开花时间调控影响产量性状研究得新进展
Yapeng Han,Qian Jia,Mengshi Liu et al.
Yapeng Han et al.
Histone demethylases serve essential functions in plant growth and development across various species. However, their roles in Glycine max remain largely unexplored. This study identified GmJMJ19 and GmJMJ20, encoding JmjC domain-containing...
Distinct steps of nuclear maturation of human pre-60S complexes require the activity of GTPases including GNL3L [0.03%]
包括GNL3L在内的GTP酶活性对于人类pre-60S复合物核成熟的不同步骤是必不可少的
Chairini C Thomé,Nicolas Lemus-Diaz,Katja I Bloch von Blottnitz et al.
Chairini C Thomé et al.
Production of the eukaryotic ribosomal subunits (40S and 60S) is a highly dynamic process in which numerous assembly factors (AFs) coordinate structural rearrangements of pre-ribosomal complexes to achieve their mature, functional architect...
RAPseq enables large-scale identification of RBP-RNA interactions and reveals essentials of post-transcriptional gene regulation [0.03%]
基于RNA的高通量测序技术能够大规模识别RNA结合蛋白与RNA相互作用并揭示其在转录后基因调控中的重要作用
Riccardo Mosca,Carlos J Gallardo-Dodd,Qun Li et al.
Riccardo Mosca et al.
Over the past decade, thousands of putative human RNA-binding proteins (RBPs) have been identified, increasing the need for methods that define their RNA-binding capacities across diverse biological settings. Existing methods rely either on...
CRISPR/Cas9 screening with destabilized bicistronic fluorescent protein reporter revealed PABPN1 as a hub of regulators for alternative polyadenylation [0.03%]
基于不稳定的双顺反子荧光蛋白报告基因的CRISPR/Cas9筛选发现PABPN1是替代多腺苷酸化调节因子的核心枢纽
Zhijie Hu,Yuchi Liu,Yuting Han et al.
Zhijie Hu et al.
Alternative polyadenylation (APA) is intricately intertwined with diverse biological processes. Efficient approaches for screening the regulatory factors of specific APA events are essential to elucidate their regulation mechanisms. Here, w...
A novel Dual-guide CRISPR-Cas13 strategy improves specificity for single-nucleotide variant detection [0.03%]
一种新型的Dual-guide CRISPR-Cas13策略可提高单核苷酸变异检测的特异性
Araceli Aguilar-González,Ismael Martos-Jamai,Iris Ramos-Hernández et al.
Araceli Aguilar-González et al.
The emergence of CRISPR-Cas systems has transformed nucleic acid detection and manipulation. Cas13, a type VI CRISPR effector, targets RNA with high sensitivity through both cis (target RNA) and trans (collateral RNA) cleavage. This propert...
Multisite phosphorylation of the AML-linked C-terminal of nucleophosmin (NPM1) orchestrates protein stability, DNA binding and charge block-driven phase separation [0.03%]
AML相关核仁磷酸蛋白C端的多泛素化调控蛋白质稳定性、DNA结合及电荷区块驱动的相分离事件
Pablo Rivero-García,Rafael L Giner-Arroyo,Joaquín Tamargo-Azpilicueta et al.
Pablo Rivero-García et al.
Nucleophosmin (NPM1) is a nucleolar protein commonly mutated in ~30% of newly diagnosed acute myeloid leukemia (AML) cases. These mutations occur in the terminal exon of the NPM1 gene, affecting the C-terminal DNA-binding domain of the prot...
Development of a competitive immunoassay platform for quantifying the oligonucleotide products of nucleotide excision repair in UV-irradiated cells [0.03%]
一种定量紫外线照射细胞中核苷酸切除修复寡核苷酸产物的免疫测定平台的发展
Geun Hoe Kim,Ha-Jeong Kwon,Youngmin Kim et al.
Geun Hoe Kim et al.
The nucleotide excision repair (NER) system removes UV photoproducts from the genome in the form of small, excised, damage-containing DNA oligonucleotides (sedDNAs). However, the methods that are traditionally used to detect these repair pr...
Iterative design of a NAND hybrid riboswitch by deep batch Bayesian optimization [0.03%]
基于深批处理贝叶斯优化的NAND杂交核糖开关迭代设计
Daniel Kelvin,Erik Kubaczka,Marianna Karava et al.
Daniel Kelvin et al.
The design of large genetic circuits requires genetic regulatory devices capable of performing complex logic operations that place no excessive metabolic burden on the host cell. Hybrid riboswitches, synthetically enhanced compact RNA eleme...
The role of the VirB ligand CTP in the molecular mechanism of transcriptional anti-silencing in Shigella flexneri [0.03%]
志贺氏菌转录去沉默的分子机制中VirB配体CTP的作用
Taylor M Gerson,Monika M A Karney,Helen J Wing
Taylor M Gerson
In bacteria, nucleoid-structuring proteins bind and constrain DNA, often leading to transcriptional silencing. In Shigella spp., the histone-like nucleoid-structuring protein H-NS silences many genes on the large virulence plasmid. Upon a s...