Reciprocal targeting of the unfolded protein response regulator Xbp1 and the DOM-A nucleosome remodeler in Drosophila [0.03%]
果蝇中未折叠蛋白反应调节因子Xbp1和DOM-A核小体重塑酶的互为目标对象
Gizem Kars,Peter B Becker,Zivkos Apostolou
Gizem Kars
The DOM-A complex regulates cell growth and proliferation in Drosophila. Like the orthologous human P400 complex, DOM-A combines two epigenetic effectors: a SWR1-type histone exchange enzyme, Dom-A, and the Tip60 acetyltransferase. We found...
Automated high-throughput selection of DNA aptamers using a common optical next-generation sequencer [0.03%]
基于下一代测序仪的DNA适配体高通量筛选方法
Alissa Drees,Christian Ahlers,Timothy Kehrer et al.
Alissa Drees et al.
Aptamers are conventionally selected via 'Systematic Evolution of Ligands by Exponential Enrichment' (SELEX). However, this process is laborious, time-consuming, and has a relatively low efficacy. In this study, we present a novel automated...
FOXN3 integrates the KU70/KU80/SREBP-1 complex to regulate lipid metabolism in non-alcoholic fatty liver disease [0.03%]
FOBN3通过整合KU70/KU80/SREBP-1复合体调节非酒精性脂肪性肝病的脂质代谢
Jiang Du,Lele Jin,Suhui Wang et al.
Jiang Du et al.
The sterol regulatory element-binding transcription factor 1 (SREBP-1) plays a crucial role in the transcriptional regulation of lipogenic response genes, thereby contributing to the development of non-alcoholic fatty liver disease (NAFLD)....
U2AF2 controls alternative splicing in speckle-proximal regions in an RS domain-dependent manner [0.03%]
U2AF2通过依赖RS结构域的方式控制斑点临近区域的可变剪接
Serhii Pankivskyi,Asaki Kobayashi,Jean de Matha Salone et al.
Serhii Pankivskyi et al.
Splicing factor U2AF2 is known to play a pivotal role for 3' splice site recognition at an early step of spliceosome assembly. Here, using proximity labeling and biochemical confirmations, we extend the repertoire of putative functional par...
Structural basis of Cas8-independent Cas3 recruitment in Type I-F2 CRISPR-Cas [0.03%]
I-F2型Cas3募集的无Cas8结构基础
Thomas Noé Perry,Christopher-Nils Mais,Mariana Sanchez-Londono et al.
Thomas Noé Perry et al.
CRISPR-Cas systems provide adaptive immunity in prokaryotes by targeting and degrading invasive genetic elements. Among them, the Type I-F2 system represents the most compact Type I CRISPR-Cas variant, distinguished by the complete absence ...
Structural basis of B-to-Z DNA transition mediated by an anti-Z-DNA antibody [0.03%]
反Z型DNA抗体介导的B型DNA向Z型DNA转换的结构基础
Cheng-Chung Lee,Shu-Fang Hsu,Ya-Wen Chang et al.
Cheng-Chung Lee et al.
Z-DNA is a left-handed double-helical form of DNA that plays roles in transcription, immune responses, viral infection, bacterial biofilm formation, and autoimmune diseases. Despite its importance, the instability of Z-DNA under physiologic...
A de novo H3.2K9me2 deposition pathway establishes heterochromatin for suppressing transposon mobilization during fly somatic development [0.03%]
一种新的H3.2K9me2沉积途径在果蝇体细胞发育过程中建立异染色质以抑制转座子动员
Yi Ni Luo,Yazi Deng,Yu Liang et al.
Yi Ni Luo et al.
Histone variants along with their associated chaperones have been considered as one of the major complexes to provide versatility in organizing chromatin structure. Post-translational modifications (PTMs) of H3 variants serve as very import...
CELF1 is a non-canonical eIF4E binding protein that promotes translation of epithelial-mesenchymal transition effector mRNAs [0.03%]
CELF1是一种非经典的eIF4E结合蛋白，可促进上皮-间充质转化效应mRNA的翻译
Arindam Chaudhury,Natee Kongchan,Shebna A Massey et al.
Arindam Chaudhury et al.
Mounting evidence is revealing an increasing complexity of gene regulation at the level of messenger RNA (mRNA) translation. Within mammalian cells, canonical cap-dependent mRNA translation depends on the eIF4F complex, consisting of the m7...
Nodavirus protein A's interdomain elbow controls RNA replication organelle formation and function [0.03%]
蛋白质A的跨界肘部控制了 nodaviruses 的RNA复制细胞器的形成和功能
Helena Jaramillo-Mesa,Megan Bracken,Hong Zhan et al.
Helena Jaramillo-Mesa et al.
Positive-strand [(+)RNA] viruses replicate their RNA genomes in poorly understood membrane-associated replication organelles (ROs). Cryo-electron microscopy of nodaviral ROs revealed that viral RNA replication protein A, with polymerase and...
Nascent transcriptome of embryonic genome activation reveals a regulatory axis linking transcriptional priming to early lineage specification in mouse embryos [0.03%]
胚胎基因激活的新生转录组揭示了小鼠胚胎早期谱系分化的转录启动与调节轴心
Yue Hu,Yuxiang Wang,Maosheng Ye et al.
Yue Hu et al.
Embryonic genome activation (EGA) marks a critical developmental transition, yet its regulatory architecture remains incompletely defined. Here, we employed optimized low-input SLAM-seq (thiol(SH)-linked alkylation for the metabolic sequenc...