Milena Bikova,Christopher T Clarkson,Vladimir B Teif
Milena Bikova
Nucleosome spacing patterns in the genome form a unique signature of a given cell, reflecting its chromatin organization and gene expression. Recently, studies of nucleosome spacing have expanded substantially due to the development of nove...
Control of retrotransposon-driven activation of the interferon response by the double-stranded RNA binding protein DGCR8 [0.03%]
双链RNA结合蛋白DGCR8控制逆转座子激活的干扰素反应
Ana Gázquez-Gutiérrez,Priscilla Chin,Guillermo Peris et al.
Ana Gázquez-Gutiérrez et al.
The type I interferon (IFN) response is the main innate immune pathway against viruses in mammals. This pathway must be tightly regulated to prevent viral spread while avoiding excessive immune responses. Here, we show that inactivation of ...
Recognition and remodelling of nucleosomes and hexasomes by the human INO80 complex [0.03%]
INO80复合物识别和重塑核小体和六聚体
Priyanka Aggarwal,Manmohan Sharma,Stephan Woike et al.
Priyanka Aggarwal et al.
The ATP-dependent INO80 chromatin remodeller slides and repositions nucleosomes to shape and maintain chromatin around gene regulatory elements and replication origins. Recent work uncovered capabilities of yeast and fungal INO80 to bind an...
HPF1 regulates the formation of FUS-dependent compartments by PARP1 and PARP2 activation on damaged DNA [0.03%]
PARP1和PARP2激活后在受损DNA上调节FUS依赖型腔室的形成
Anastasia S Singatulina,Maria V Sukhanova,Loic Hamon et al.
Anastasia S Singatulina et al.
FUS participates in the formation of biomolecular condensates associated with PARP1-dependent synthesis of poly(ADP-ribose) (PAR). HPF1 regulates auto- and hetero-PARylation activities of PARP1 and PARP2 and may influence the formation of F...
Caffeine inhibits nonhomologous end joining by impairing ligase IV/XRCC4 function [0.03%]
咖啡因通过破坏DNA连接酶IV/XRCC4的功能来抑制非同源末端连接作用
Susmita Kumari,Divya Sathees,Prashant Kumar Rai et al.
Susmita Kumari et al.
DNA double-strand breaks (DSBs), the most lethal DNA lesions, are repaired primarily by homologous recombination (HR) or nonhomologous end joining (NHEJ). Caffeine is known to inhibit HR by displacing Rad51 from single-stranded DNA, but its...
TDG orchestrates ATF4-dependent gene transcription during retinoic acid-induced cell fate acquisition [0.03%]
TDG通过调控ATF4依赖性基因转录介导视黄酸诱导的细胞命运决定
Marion Turpin,Thierry Madigou,Maud Bizot et al.
Marion Turpin et al.
Acquisition of cell identity is associated with a remodeling of the epigenome in part through active DNA demethylation. The T:G mismatch DNA glycosylase (TDG) participates to this process by removing 5-methylcytosines that have been oxidize...
A tailored phosphorothioate coordinator enables CRISPR/Cas in-situ amplification [0.03%]
一种定制型磷硫酰基配体使CRISPR/Cas能够进行原位扩增
Tiantian Yang,Man Tang,Li Xu et al.
Tiantian Yang et al.
The CRISPR/Cas system is a powerful tool for molecular diagnostics, but its reliance on linear amplification constrains sensitivity, particularly for in situ imaging. Here, we discovered that phosphorothioate (PS)-modified activators can mo...
Xiaochen Liang,Ting Zhao,Xiaoxia Dai et al.
Xiaochen Liang et al.
Nucleotide repeat expansions contribute to a number of neurological disorders. Mutations and augmented expression in fused in sarcoma (FUS) can result in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). Here...
Functional decoupling of crRNA enables customizable CRISPR diagnostics [0.03%]
功能性解耦crRNA可实现可定制的CRISPR诊断功能
Hyungbin Park,Jiyoung Yun,Kyuhan Lee et al.
Hyungbin Park et al.
One-pot CRISPR-based diagnostics have transformed nucleic acid testing, yet their design customizability remains constrained. Because target programming and cis-cleavage activity are simultaneously determined during CRISPR RNA (crRNA) desig...
Zejia Li,Chaoyang Xiong,Jin Shen et al.
Zejia Li et al.
The suppression of transposable elements in the germline is critical for safeguarding fertility. Here we identified that DAXX (death-associated protein 6), a potent transcription repressor and an H3.3 chaperone, was indispensable for silenc...