Cryo-EM structures reveal a conserved architecture for raiA noncoding RNA [0.03%]
低温电子显微镜结构揭示了 RaiA 非编码RNA的保守构象
Yao He,Janet Zhong,Yuan Yang et al.
Yao He et al.
RaiA motif RNA is a family of bacterial noncoding RNAs (ncRNAs) found in over 2700 bacterial species. Although its cellular abundance is comparable to that of rRNAs and tRNAs in the human pathogen Clostridioides difficile and its knockout r...
3D-super-enhancers are condensate-associated cis-regulatory communities [0.03%]
三维超级增强子是与凝聚体相关的顺式调控社区
Jie Lv,Kelsey A Maher,Li Dong et al.
Jie Lv et al.
Transcription proteins are concentrated at nuclear transcriptional condensates. These condensates contain cis-regulatory elements (CREs), including enhancers and promoters, that are thought to regulate genes in the same condensate. The role...
LysR-type regulator LrhA promotes CRISPR-Cas immunity in Escherichia coli [0.03%]
LysR型调节因子LrhA通过促进CRISPR-Cas免疫系统在大肠杆菌中的表达来抵抗噬菌体感染
Mengdie Fang,Jim Yap,Mingyue Fei et al.
Mengdie Fang et al.
The CRISPR-Cas defense system safeguards prokaryotes against foreign genetic elements. Its activity is determined by the combined effects of adaptation and interference. However, the dynamic regulation of these two processes remains not ful...
A retroelement-derived mammalian ARC protein exhibits selective RNA recognition and nucleic acid chaperone functions [0.03%]
由逆转录元件衍生的哺乳动物ARC蛋白表现出选择性RNA识别和核酸伴侣功能
Julita Gumna-Mikina,Angelika Andrzejewska-Romanowska,Maciej Antczak et al.
Julita Gumna-Mikina et al.
Activity-regulated cytoskeleton-associated protein (ARC) is an RNA-binding protein that also serves as a central hub for neuronal protein-protein interactions. It is essential for intercellular signaling and contributes to synaptic plastici...
Li Wen,Mauricio A Arias,Xinqi Fan et al.
Li Wen et al.
Nuclear speckles are membraneless organelles implicated in multiple RNA processing steps. In this work, we systematically characterize the sequence logic determining RNA localization to nuclear speckles. We find extensive similarities betwe...
Regulatory paradigm of Dscam1 stochastic alternative splicing through conserved long-range RNA structures [0.03%]
Dscam1随机选择性拼接的调控范式及其保守的长程RNA结构
Haiyang Dong,Bingbing Xu,Lili Wu et al.
Haiyang Dong et al.
Pancrustacean Dscam1 genes encode 2 000-120 000 distinct isoforms via mutually exclusive splicing; however, the underlying regulatory mechanisms are not fully understood. Here, we revealed a regulatory paradigm of stochastic Dscam1 alternat...
DeepECC: a deep learning framework for genome-wide identification and analysis of human cancer eccDNAs [0.03%]
DeepECC：一种用于全基因组鉴定和分析人类癌症eccDNA的深度学习框架
Changcheng Wang,Yisen Xu,Rufeng Li et al.
Changcheng Wang et al.
Extrachromosomal circular DNAs (eccDNAs) are closed circular DNA molecules widespread across eukaryotic cells, with emerging roles in gene regulation and tumor progression. Experimental assays remain costly and incomplete, underscoring the ...
The molecular mechanisms of the ShosTA system in mediating anti-phage defense [0.03%]
ShosTA系统介导抗噬菌体防御的分子机制
Rong Yang,Libang He,Zhuoxi Wu et al.
Rong Yang et al.
The ShosTA system, a two-component toxin-antitoxin (TA) system consisting of the ShosT and ShosA proteins, has recently been shown to mediate anti-phage defense. However, the molecular mechanisms underlying this system's role in anti-phage ...
crRNA scaffold remodeling controls CRISPR-Cas12a activity for enhanced performance [0.03%]
通过CRISPR-Cas12a活性控制实现增强性能的crRNA支架重构
Zhongzhong Wang,Yi Wu,Zixuan Wang et al.
Zhongzhong Wang et al.
CRISPR-Cas12a has transformative potential in molecular diagnostics owing to its robust signal amplification, but its sustained activity state severely limits temporal programmability and precise nuclease control in complex detection workfl...