MexR and NalD are pyocyanin-responsive transcriptional repressors of the efflux pump MexAB-OprM in Pseudomonas aeruginosa [0.03%]
荧光素酶报告基因检测技术研究沙雷菌属细菌毒力基因的转录调控网络
Cancan Wu,Maofeng Wang,Yumeng Xiao et al.
Cancan Wu et al.
The secondary metabolite pyocyanin (PYO) significantly enhances the antibiotic resistance of Pseudomonas aeruginosa by inducing expression of the resistance-nodulation-cell division efflux systems. However, the mechanisms underlying the ini...
Unlocking genetic potential: harnessing phage for targeted mutagenesis in phage-assisted evolution [0.03%]
释放遗传潜力：利用噬菌体进行噬菌体辅助进化的定向突变
Maryam Ali,Afnan Khan,Thandava Vanapilli Nursimulu et al.
Maryam Ali et al.
A critical challenge in DNA library genesis for evolution systems is avoiding off-target mutations, which can introduce undesirable changes elsewhere in the plasmid or even the host's genome: such mutations can allow cells or phages to prop...
Structural basis for small molecule binding to the SARS-CoV-2 nsp10-nsp14 ExoN complex [0.03%]
SARS-CoV-2 nsp10-nsp14 ExoN 复合物与小分子结合的结构基础
Frank Kozielski,Suzanne Zoë Fisher,Shumeng Ma et al.
Frank Kozielski et al.
Coronavirus outbreaks have occurred over the past 25 years with SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2) causing a global pandemic. The SARS-CoV-2 non-structural proteins 10 (nsp10) and 14 (nsp14) are considered as poten...
Cyclic tetraadenylate binding induces dimerization of protein dimers to activate a CRISPR-associated PIN nuclease [0.03%]
环状四腺苷酸结合诱导蛋白二聚体二聚化以激活CRISPR相关PIN内切酶
Fang Wang,Pengpeng Zhao,Xiaonan Bi et al.
Fang Wang et al.
Type III CRISPR-Cas systems synthesize cyclic oligoadenylates (cOAs), the second messengers that bind to the CARF (CRISPR-associated Rossman fold) sensor domain and allosterically activate the effector domain of CRISPR ancillary effectors t...
A versatile anti-CRISPR platform for opto- and chemogenetic control of CRISPR-Cas9 and Cas12 across a wide range of orthologs [0.03%]
一种通用的反CRISPR平台，可对多种同源物的CRISPR-Cas9和Cas12进行光控和化学遗传控制
Luca Brenker,Sabine Aschenbrenner,Felix Bubeck et al.
Luca Brenker et al.
CRISPR-Cas technologies have revolutionized life sciences by enabling programmable genome editing across diverse organisms. Achieving dynamic and precise control over CRISPR-Cas activity with exogenous triggers, such as light or chemical li...
Xingjian Bai,Jack F Hong,Shan Yu et al.
Xingjian Bai et al.
Plasmids are indispensable in life sciences research and therapeutics development. Currently, most labs custom-build their plasmids. As yet, no systematic data on the quality of lab-made plasmids exist. Here, we report a broad survey of pla...
Quantitative modeling of mRNA degradation reveals tempo-dependent mRNA clearance in early embryos [0.03%]
定量分析mRNA降解发现早期胚胎中转录本的清除速率取决于其存在时间
Mazal Tawil,Dina Alcalay,Pnina Greenberg et al.
Mazal Tawil et al.
As embryos transition from maternal to zygotic control, precise clearance of pre-loaded maternal mRNAs is essential for initiating new zygotic gene expression programs. Yet the kinetics of this process and how it adapts across different dev...
Trichothiodystrophy-causative pathogenic variants impair a cooperative action of TFIIH and DDX1 in R-loop processing [0.03%]
末端螺旋毛发稀疏-智力障碍综合征致病基因变异损害TFIIH与DDX1在R环处理中的协同作用
Debora Ferri,Giulia Branca,Manuela Lanzafame et al.
Debora Ferri et al.
The transcription factor IIH (TFIIH) is a key player in transcription and DNA repair by nucleotide excision repair. It is made of 10 subunits organized in core-TFIIH and CAK sub-complexes bridged by XPD. Pathogenic variants in the ERCC2/XPD...
Capturing a glycosylase reaction intermediate in DNA repair by freeze-trapping of a pH-responsive hOGG1 mutant [0.03%]
利用pH响应型hOGG1突变体的冻结捕捉捕获DNA修复过程中碱基切除修复酶氧化鸟嘌呤DNA糖基化酶反应中间态结构的研究
Masaki Unno,Masayuki Morikawa,Vladimír Sychrovský et al.
Masaki Unno et al.
The human 8-oxoguanine DNA glycosylase 1 (hOGG1) is a bifunctional DNA repair enzyme that possesses both glycosylase and AP-lyase activity. Its AP-lyase reaction mechanism had been revealed by crystallographic capturing of the intermediate ...