Human DNA polymerase η is regulated by mutually exclusive mono-ubiquitination and mono-NEDDylation [0.03%]
人类DNA聚合酶η通过相互排斥的单泛素化和单NEDD8化受到调节
Natália C Moreno,Emilie J Korchak,Marcela T Latancia et al.
Natália C Moreno et al.
DNA polymerase eta (Pol η) is a Y-family translesion polymerase responsible for synthesizing new DNA across UV-damaged templates. It is recruited to replication forks following mono-ubiquitination of the PCNA DNA clamp. This interaction is...
A novel two-component system regulates shikimate pathway for thermal adaptation in Thermus thermophilus [0.03%]
一个新的二元系统调节热休克蛋白在嗜热栖热菌中的莽草酸途径以适应高温
Xuying Bu,Yuwen Guo,Xiangyu Tian et al.
Xuying Bu et al.
Temperature stress is a fundamental challenge for all organisms. While two-component systems (TCSs) are known to transduce environmental signals in microbes, their role in thermal sensing remains underexplored. Here, we unveil a novel therm...
High-throughput mapping of spontaneous mitotic crossover and genome instability events with sci-L3-Strand-seq [0.03%]
一种使用sci-L3-strand-seq高通量绘制自发性有丝分裂重组和基因组不稳定性事件的方法
Peter Chovanec,Trevor Ridgley,Yi Yin
Peter Chovanec
Despite the many advances in single cell genomics, detecting structural rearrangements in single cells, particularly error-free sister-chromatid exchanges, remains challenging. Here we describe sci-L3-Strand-seq, a combinatorial indexing me...
Molecular interactome of HNRNPU reveals regulatory networks in neuronal differentiation and DNA methylation [0.03%]
HNRNPU分子互作网络揭示其在神经元分化和DNA甲基化中的调控作用
Marika Oksanen,Francesca Mastropasqua,Krystyna Mazan-Mamczarz et al.
Marika Oksanen et al.
HNRNPU is an RNA-binding protein with diverse roles in transcriptional and post-transcriptional regulation. Pathogenic genetic variants of HNRNPU cause a severe neurodevelopmental disorder (NDD), but the underlying molecular mechanisms are ...
Machine learning-optimized long single-stranded DNA synthesis technology empowers high-precision diagnostic-therapeutic integration in living cells [0.03%]
机器学习优化的长单链DNA合成技术赋能活细胞精准检测治疗一体化技术
Yue Zhang,Yiming Xu,Zhihua Ding et al.
Yue Zhang et al.
While DNA nanotechnology holds transformative potential across biomedical and information storage applications, current technologies face critical limitations in synthesizing long single-stranded DNA (ssDNA) with high purity and homogeneity...
High-throughput measurement and prediction of the i-motif DNA stability landscape [0.03%]
高效测量和预测i-动机DNA稳定性景观
Chengjie Chu,Shilong Zhang,Ziyue Guan et al.
Chengjie Chu et al.
The i-motif, a cytosine-rich DNA secondary structure, exhibits pH- and temperature-dependent stability. We developed a high-throughput, five-dimensional ultraviolet melting/annealing (5DUVMA) method to systematically probe these dependencie...
Structure, function, and applications of two novel phage recombinases from extreme environments [0.03%]
极端环境下两种新型噬菌体重组酶的结构、功能及应用
Emma Tarrant,Isabel G Cormack,Charlotte E Hunter et al.
Emma Tarrant et al.
This study describes the identification and characterization of two new extremophilic phage recombinases, UvsXt and UvsXp, discovered through metagenomic analysis within the Virus-X project, and explores their potential applications in biot...
Azoospermia phenotype and scRNA-seq reveal hnRNPK as a factor essential for male germ cell development in mice [0.03%]
无精子表型和scRNA-seq揭示hnRNPK是小鼠雄性生殖细胞发育中的关键因子
Huihui Gao,Shiyu Yang,Ao Ning et al.
Huihui Gao et al.
The process of male germ cell development is a central determinant of spermatogenesis. Nevertheless, the genetic regulatory mechanisms underlying male germ cell development in mammals remain largely unclear. In this study, employing a germ ...
Scaffolding of the H4K5ac chromatin remodeling complex by lncRNA MAHAC mediates epithelial-mesenchymal transition [0.03%]
长链非编码RNA MAHAC通过搭建H4K5乙酰化染色质重塑复合物介导上皮间充质转化
Kai-Wen Hsu,Jeng-Shou Chang,Joseph Chieh-Yu Lai et al.
Kai-Wen Hsu et al.
Anchoring of a chromatin remodeler complex by long non-coding RNAs (lncRNAs) is a frequently utilized mechanism for lncRNAs to regulate gene expression. Hypoxia is a microenvironmental condition that plays a crucial role in promoting tumor ...