Takamasa Hashizume,Koki Baba,Naoya Matsuo et al.
Takamasa Hashizume et al.
Monoclonal antibodies (mAbs) are key therapeutics for diseases like cancer and autoimmunity. The production of mAbs relies on cell culture, in which the culture medium for high productivity and activity is essential. Despite the traditional...
Functionalized peptide hydrogel to generate human insulin-producing cells in vitro [0.03%]
用于体外生成人胰岛素产生细胞的功能化肽水凝胶
Brandhon F Flores-Ibarra,Andrea I Enríquez-Rodríguez,Kimberly P Robles-Pablos et al.
Brandhon F Flores-Ibarra et al.
Type I diabetes is a chronic disease that affects people worldwide. When insulin administration is no longer effective, transplantation of pancreatic islets represents an alternative for diabetics. However, islet grafting carries limitation...
Production and characterization of norovirus virus-like particles vaccine candidates in a genetically modified Ogataea minuta system [0.03%]
一种用于口服的诺如病毒候选疫苗颗粒型病毒样颗粒在基因改造Ogataea minuta系统中的生成和鉴定研究
Masashi Tsuda,Yuki Nakatani,Satoshi Baba et al.
Masashi Tsuda et al.
The development of an effective vaccine against noroviruses remains a major public health priority. Norovirus GII.4 capsid protein VP1 as a promising vaccine candidate was produced by the methylotrophic yeast Ogataea minuta production syste...
Spheroid size-induced apoptosis enhances osteogenic differentiation of iPS cells [0.03%]
类球体大小诱导的凋亡增强诱导多能干细胞成骨分化
Hideto Tatsumi,Hiroko Okawa,Naruephorn Vinaikosol et al.
Hideto Tatsumi et al.
Induced pluripotent stem cells (iPSCs) have significant potential for regenerative medicine, particularly for bone tissue engineering. While three-dimensional spheroid cultures enhance iPSC differentiation by better mimicking physiological ...
Establishment of a simple and novel method for contact-dependent intercellular interaction analysis using extracellular vesicles [0.03%]
建立一种使用细胞外囊泡进行接触依赖性细胞间相互作用分析的简单而新颖的方法
Rina Sakamaki,Takao Matsuba,Yasuyuki Kurihara
Rina Sakamaki
Extracellular vesicles (EVs), including exosomes, microvesicles, and apoptotic bodies, are membrane-bound vesicles secreted by cells. They play essential roles in intercellular communications and are involved in numerous physiological proce...
Adaptive laboratory evolution optimizes an engineered phosphite utilization pathway in Synechococcus elongatus PCC 7942 [0.03%]
适应性实验室进化优化了Synechococcus elongatus PCC 7942中工程化磷ites利用途径
Hiroki Murakami,Naoki Momokawa,Kei Motomura et al.
Hiroki Murakami et al.
Synthetic biology approaches enable the creation of promising chassis for practical application in various fields, though engineering of microbial metabolism often imposes a metabolic burden, potentially driving adaptive evolution during lo...
Development of dectin-1-binding peptides targeting dendritic cells for antigen delivery via ribosome display [0.03%]
基于核糖体显示技术筛选树突细胞特异性结合的Dectin-1肽拮抗剂用于抗原递呈
Yoshirou Kawaguchi,Md Shahin Sarker,Mina Yokoyama et al.
Yoshirou Kawaguchi et al.
Dendritic cells (DCs) are professional antigen-presenting cells that play a central role in initiating and shaping adaptive immune responses. Targeting antigens to DCs has emerged as a promising strategy to enhance vaccine efficacy and tail...
Nevin McCone,Masahito Hosokawa
Nevin McCone
Single-cell genomics (SCG) complements culture-independent metagenomics for accessing fungal genomes, particularly from lineages that remain uncultured. We contrast metagenomics, which excels when profiling community composition and metabol...
Zein/Fucoidan microcarriers promote myogenic differentiation via topographical cues and hydrodynamic modulation [0.03%]
苔藓虫多糖/角蛋白微载体促进成肌分化的拓扑和流体动力学调节作用
Wanli Xiong,Chengxin Ge,Botao Zhang et al.
Wanli Xiong et al.
Microcarrier surface topography and fluid shear stress (FSS) critically regulate cellular behavior. An edible zein/fucoidan microcarrier with 200 μm grooves was designed for this study. Computational fluid dynamics (CFD) simulations and ex...
Spatial, temporal, and biotic changes in the natural abundances of stable carbon and nitrogen isotopes in a biological treatment reactor [0.03%]
生物处理反应器中稳定碳氮同位素自然丰度的时空和生物变化规律
Takashi Onodera,Kengo Kubota,Akinori Iguchi et al.
Takashi Onodera et al.
The natural abundances of stable isotopes were used to determine prey-predator relationships and material flows in a biological treatment reactor for municipal wastewater. The reactor used in this study was a down-flow hanging sponge (DHS),...