Comparison of long-term aged Japanese sake and typical aged liquors in the world [0.03%]
日本清酒长期熟成与世界传统蒸馏酒的比较研究
Ryotaro Hori,Yukio Osafune,Yuriko Ikeda et al.
Ryotaro Hori et al.
Recently, sake aged for several years to decades, called long-term aged sake, has attracted some attention. Aged liquors also exist in other countries, such as Chinese rice wine (Huangjiu) and fortified wines (Sherry, Madeira, Port). To und...
Evaluation of cellular characteristics and genome editing responses in chicken primordial germ cell lines [0.03%]
鸡原始生殖细胞系的细胞特性评估及基因组编辑反应分析
Yuya Kaneko,Yoshinori Kawabe,Ken-Ichi Nishijima et al.
Yuya Kaneko et al.
Chicken primordial germ cells (cPGCs) hold great potential for genetic modification and germ cell research in chickens. In this study, we evaluated the cellular characteristics of three cPGC lines: cPGC-1, cPGC-2, and cPGC-3. cPGC-1 and cPG...
Metabolic pathway of the rare sugar 5-keto-d-fructose in the oleaginous yeast Lipomycesstarkeyi [0.03%]
施氏假丝酵母中稀有糖类5-酮基-D-果糖的代谢路径
Yukie Noyori,Rikako Sato,Kei Takeshita et al.
Yukie Noyori et al.
Acetic acid bacteria convert environmental sugars and alcohols into acetic acid and various sugars through oxidative fermentation, resulting in the accumulation of these compounds at high concentrations in the culture medium. One such produ...
Single-cell ATP monitoring in Escherichia coli engineered for polyhydroxybutyrate production using the fluorescent biosensor QUEEN [0.03%]
利用荧光生物传感器QUEEN监测工程产聚羟基丁酸酯大肠杆菌的单细胞ATP水平
Wataru Fuji,Ayaka Kajikawa,Shin-Ichi Hachisuka et al.
Wataru Fuji et al.
Polyhydroxyalkanoates (PHAs) are polyesters accumulated by various microorganisms as intracellular inclusions. In this study, Escherichia coli was engineered to co-express PHA biosynthetic genes and the ratiometric fluorescent biosensor QUE...
Single-cell transcriptomic profiling of platelet-adherent circulating tumor cells using a microcavity-gel manipulation platform [0.03%]
利用微腔凝胶操控平台对血小板黏附的循环肿瘤细胞进行单细胞转录组分析
Tomoko Yoshino,Sakurako Sato,Marshila Kaha et al.
Tomoko Yoshino et al.
Pancreatic cancer remains one of the most lethal malignancies, with a 5-year survival rate of less than 9 %, primarily due to its aggressive metastasis and drug resistance. Circulating tumor cells (CTCs), which are key mediators of metastas...
Characterization of flavor quality in sago starch produced by different processing methods: Insights from physicochemical, metabolomic, and sensory analyses [0.03%]
不同加工方法生产的手粉的风味品质特征：来自理化、代谢组学和感官分析的见解
Giri R Barokah,Azis B Sitanggang,Eiichiro Fukusaki et al.
Giri R Barokah et al.
Sago is a high-carbohydrate, naturally gluten-free product derived from tropical palm trees, serving as an essential staple food in many regions of Southeast Asia and the Pacific Islands. The various processing methods used to produce sago ...
Development of monoclonal antibody targeting membrane protein utilizing modified Ecobody technology [0.03%]
利用改良的Ecobody技术开发针对膜蛋白的单克隆抗体
Daffa Sean Adinegoro,Teruyo Ojima-Kato,Hideo Nakano
Daffa Sean Adinegoro
This study presents a preliminary report on the development of rabbit monoclonal antibodies targeting the extracellular domain of rat G protein-coupled receptor 54 (GPR54). The extracellular domain of GPR54 (GPR54 ECD) was fused with the al...
Biosynthesis of poly(6-hydroxyhexanoate) [poly(ε-caprolactone)] using engineered polyhydroxyalkanoate synthetic system in Escherichia coli [0.03%]
工程聚羟基烷酸合成系统在大肠杆菌中合成聚（6-羟己酸）[聚（己内酯）]
Kengo Yanagawa,Shin-Ichi Hachisuka,Haruno Kusumoto et al.
Kengo Yanagawa et al.
Poly(ε-caprolactone) (PCL), also known as poly(6-hydroxyhexanoate) [P(6HHx)], is a biodegradable polyester characterized by excellent flexibility, processability, and marine degradability, making it a promising alternative to conventional ...
TNF-α-induced contractile dysfunction in three-dimensional engineered muscle [0.03%]
肿瘤坏死因子所致工程化三维肌肉组织的收缩功能障碍
Yukinori Tamura,Junpei Ishizaka,Sho Yokoyama et al.
Yukinori Tamura et al.
Three-dimensional engineered muscle (3D-EM) provides a physiologically relevant model for examining skeletal muscle function. Tumor necrosis factor-α (TNF-α), a pro-inflammatory cytokine elevated in chronic conditions such as sarcopenia a...
Identification and overexpression of genes encoding sugar alcohol transporter and metabolic enzymes for accelerated utilization in the yeast Kluyveromyces marxianus [0.03%]
在酵母马克斯克鲁维酵母中鉴定并过表达糖醇转运蛋白和代谢酶基因以加速其利用效率
Satoshi Ebe,Hitomi Nakamura,Mitsunari Matsuda et al.
Satoshi Ebe et al.
The yeast Kluyveromyces marxianus assimilates various sugars, including sorbitol and mannitol. However, the metabolic pathways for sugar utilization, including sugar transporters, remain to be elucidated. To identify these genes in this stu...