Identification of DeuA, an Aspergillus oryzae-derived deuterolysin-like metalloprotease, as the predominant thermostable protease in soy sauce [0.03%]
从酱油曲霉中鉴定到DeuA，一种新型的耐热金属蛋白酶
Taro Matsuoka,Ken Oda,Kazuhiro Iwashita et al.
Taro Matsuoka et al.
The thermostable proteases present in soy sauce can degrade proteins in processed foods, such as boiled eggs and fish cakes, leading to undesirable textural changes in the product. In this study, we identified DeuA, a deuterolysin-like meta...
Reprogramming the flavo-encapsulin from Thermotoga maritima to a novel protein nanocage platform for potent photodynamic therapy [0.03%]
大马铃薯海洋栖热菌的着色剂包氯素重构为新型蛋白纳米笼平台用于光动力治疗
Ryutaro Nakamura,Mamoru Nonaka,Fumihiko Nakamura et al.
Ryutaro Nakamura et al.
Encapsulins are protein nanocages consisting of a single monomer protein and have gained increasing interest as a platform for diverse biological nanotools due to their ease of production and robust stability. We found that the Thermotoga m...
Structure-guided engineering of substrate specificity in yeast d-aspartate oxidase [0.03%]
基于结构的工程改造酵母D-天冬氨酸氧化酶底物特异性
Sota Zaitsu,Daiki Imanishi,Shouji Takahashi
Sota Zaitsu
Selective quantification of individual d-amino acids is crucial for functional studies and biomarker development. Enzymatic assays enable convenient and rapid detection of d-amino acids. d-Amino acid oxidases (DAAOs) and d-aspartate oxidase...
Fetal bovine serum concentration-dependent differentiation of multi-layered myoblasts [0.03%]
浓度依赖性的牛血清诱导多层肌前体细胞分化
Eiji Nagamori,Hiroki Nagatani,Koichi Sugita et al.
Eiji Nagamori et al.
Skeletal muscle tissue engineering has gained significant attention for applications in drug screening and regenerative medicine. Among the available approaches for engineering skeletal muscle tissue, lamination of cell sheets is particular...
Metabolomics-based investigation of compounds associated with sensory profiles of strawberries [0.03%]
基于代谢组学的草莓风味化合物研究
Yuri Yoshiyama,Taichiro Fujimura,Masahiro Furuno et al.
Yuri Yoshiyama et al.
Strawberry cultivars have recently been diversified, and breeding objectives generally focus on production characteristics, as well as those to attract consumers. Strawberry cultivar selection and breeding rely heavily on human sensory eval...
Utilization of biocatalysts in an artificial photosynthesis system: A biotechnological approach towards sustainable development [0.03%]
生物催化剂在人工光合作系统中的应用——面向可持续发展的生物技术方法论研究
Harshita Shringi,Ressin Varghese,Andrews Nirmala Grace et al.
Harshita Shringi et al.
Harnessing solar energy more efficiently and sustainably remains a key challenge in advancing renewable, bio-based production systems. Artificial photosynthesis tends to mimic natural photosynthesis by using catalytic systems and semiconduc...
Mineralization-driven cellular dynamics and tissue remodeling in scaffold-free MC3T3-E1 spheroids [0.03%]
无支架MC3T3-E1细胞球状体中矿化驱动的细胞动力学及组织重塑现象研究
Jeonghyun Kim,Ryotaro Ikebe,Eijiro Maeda et al.
Jeonghyun Kim et al.
Bone mineralization is a critical process in bone tissue formation and remodeling, yet the underlying mechanisms in three-dimensional (3D) cellular environments remain unclear. In this study, we fabricated scaffold-free spheroids using mous...
Synthetic transactivator-promoter systems for exogenous gene expression in Chlamydomonas reinhardtii [0.03%]
用于绿藻衣原体重组基因表达的合成反式激活因子-启动子系统
Yoshinori Kawabe,Tatsuki Akiyama,Kokoro Miyazoe et al.
Yoshinori Kawabe et al.
Chlamydomonas reinhardtii, which is a unicellular photosynthetic eukaryote, has long served as a model microalga for fundamental biological research and biotechnological applications. Recently, it has attracted attention as a promising biol...
Occasional production by genome-wide deletion through removal of linear plasmid harboring tap-tpg gene pair in Streptomycesrochei [0.03%]
大环内酯类抗生素轮枝菌素的产生：轮枝菌Tap-Tpg基因对失活导致的群体感应缺陷所致
Mingge Zhang,Yosi Nindita,Amirudin Akhmad Fauzi et al.
Mingge Zhang et al.
Streptomyces rochei 7434AN4 carries three linear plasmids, pSLA2-L (211 kb), pSLA2-M (113 kb), and pSLA2-S (18 kb). Genome project of this strain revealed that the linear chromosome consists of 8.36 Mb in size and carries more than 35 biosy...
Enhancing sensory quality of heat-not-burn tobacco via bioaugmentation with Cytobacillus oceanisediminis C4 and amylase [0.03%]
海洋来源的Cytobacillus oceanisediminis C4和淀粉酶生物强化加工可加热不燃烧烟草感官品质研究
Li-Yuan Zhang,Ying Ning,Bin-Bin Hu et al.
Li-Yuan Zhang et al.
Tobacco fermentation is a crucial process for improving tobacco quality by reducing undesirable substances and enhancing aroma characteristics. In this study, an enzyme-microbe combined fermentation strategy was established and evaluated. C...