Genetic interaction between the septation initiation network component SepM and the cell wall integrity transcription factor RlmA regulates cellobiose-responsive CAZyme expression in Aspergillus aculeatus [0.03%]
Aspergillus aculeatus中的分离启动网络成分SepM与细胞壁完整性转录因子RlmA的遗传相互作用调控鼠李糖枝孢霉中的半乳糖苷酶表达
Yui Shiga,Kazumi Sawada,Saki Kikuya et al.
Yui Shiga et al.
We previously reported that SepL and SepM, components of the septation initiation network (SIN) complex, are involved in the activation of the ManR-dependent cellobiose-responsive expression in the cellulase-producing fungus Aspergillus acu...
Characterization of a bioflocculant produced by lactic acid bacterium Leuconostoc mesenteroides NBRC 100496 [0.03%]
由乳酸菌肠膜明串珠菌NBRC 100496产生的一种生物絮凝剂的性质研究
Nozomi Hasunuma,Shigekazu Yano,Yuri Tamaki et al.
Nozomi Hasunuma et al.
Extracellular polymers produced by microorganisms are utilized as bioflocculants. In this study, bioflocculant-producing lactic acid bacteria were screened from seven strains based on the flocculation activity of their cultures. The Leucono...
Selective rewiring of Met4 ubiquitin regulation enhances S-adenosylmethionine accumulation in sake yeast [0.03%]
通过选择性重新接线Met4泛素调节机制增强清酒酵母S-腺苷甲硫氨酸积累
Shogo Kakoi,Akira Nishimura,Hiroyuki Senjyu et al.
Shogo Kakoi et al.
S-Adenosylmethionine (SAM) is a central sulfur-containing metabolite that serves as a universal methyl-group donor and a high-value molecule for food and pharmaceutical applications. In Saccharomyces cerevisiae, sulfate assimilation into me...
Biochemical and structural characterization of a guaiacol O-methyltransferase from Magnolia salicifolia [0.03%]
Magnolia salicifolia木荷树酚O-甲基转移酶的生物化学及结构研究
Kanon Goto,Shintaro Maeno,Seichi Suzuki et al.
Kanon Goto et al.
Volatile benzenoids such as veratrole and methyleugenol, together with terpenoids, constitute the characteristic floral scent of anise magnolia (Magnolia salicifolia), and the composition of these volatiles varies among natural populations ...
Anti-inflammatory activity of 3″-hydroxyisoxsuprine, a catechol-structured product of biotransformation hydroxylation, in lipopolysaccharide-stimulated RAW 264.7 macrophage cells [0.03%]
3″-羟基异氧普林通过生物转化羟基化产生的一种儿茶酚结构产物在脂多糖刺激RAW264.7巨噬细胞中的抗炎活性研究
Chien-Yu Wu,Jiumn-Yih Wu,Huei-Ju Ting et al.
Chien-Yu Wu et al.
In our previous work, we used a predicted data mining approach (PDMA) to identify biotransformable precursors. This enabled the successful synthesis of 3″-hydroxyisoxsuprine, a catechol-structured compound, from isoxsuprine hydrochloride (...
Facile inactivation of a Streptomyces global regulator using a versatile base editing plasmid for secondary metabolism perturbation across multiple species [0.03%]
一种用于多种物种次级代谢扰动的放线单胞菌全局调控因子便捷失活的碱基编辑质粒
Ryo Otsuka,Hiroya Tomita,Kentaro Miyazaki et al.
Ryo Otsuka et al.
Members of the genus Streptomyces produce a wide array of bioactive and structurally diverse secondary metabolites. However, many biosynthetic gene clusters (BGCs) remain silent (cryptic) under standard laboratory cultivation conditions, po...
Engineering of Komagataella phaffii for enhanced d-lactic acid production via deletion of a cell wall stability-related gene and optimization of cultivation conditions [0.03%]
通过删除细胞壁稳定性相关基因并优化培养条件来工程改造Komagataella phaffii以增强D-乳酸生产
Yoshifumi Inoue,Yohei Koya,Ryosuke Yamada et al.
Yoshifumi Inoue et al.
Methanol has attracted increasing attention as a sustainable one-carbon feedstock because it can be produced from carbon dioxide and hydrogen or from waste biomass. Additionally, it can be economically stored and transported as a liquid. Th...
NctA, a CBF/NF-Y family transcriptional factor, is involved in secreted proteins production in liquid and solid-state culture in Aspergillusoryzae [0.03%]
丝状真菌中CBF/NF-Y家族转录因子Ncta通过调控分泌蛋白参与液体和固体发酵中的次级代谢产物合成
Gen-Ya Arakawa,Masahiro Ogawa,Masafumi Tokuoka
Gen-Ya Arakawa
Aspergillus oryzae is an industrially important filamentous fungus used for food fermentation in Japan. It secretes amylolytic and proteolytic enzymes essential for the fermentation process. Understanding the mechanisms regulating amylolyti...
Microscale solid-state cultivation for evaluating enzyme productivity in filamentous fungi [0.03%]
丝状真菌微尺度固态培养法评估酶产量
Shimma Fujiwa,Ayaka Kinumi,Tomohiko Matsuzawa
Shimma Fujiwa
Filamentous fungi, such as Aspergillus oryzae, A. luchuensis, and Trichoderma reesei, produce many types of enzymes, including glycoside hydrolases and proteases, which are important for brewing and biotechnological applications. Solid-stat...
Near-infrared imaging-based high-content analysis for label-free assessment of internal cellular heterogeneity in spheroid [0.03%]
基于近红外成像的高内涵分析方法实现无标记评估类器官球内部细胞异质性
Ren Sakai,Kenjiro Tanaka,Miya Kawasaki et al.
Ren Sakai et al.
Non-invasive analysis of spheroid quality was essential because spheroids better recapitulated in vivo-like cell-cell and cell-extracellular matrix (ECM) interactions than two-dimensional cultures. However, routine non-invasive methods to a...