An optimized protocol for identifying S-acylated proteins in Dictyostelium discoideum [0.03%]
优化的Dictyostelium discoideum中识别S-酰化蛋白质协议
Samer A Owiar,William David Kim,Robert Joseph Huber
Samer A Owiar
S-palmitoylation is a reversible post-translational modification that adds palmitic acid onto cysteine residues of proteins through the formation of a thioester bond. The reaction is catalyzed by protein acyl transferases (PATs) and reverse...
Fan Du,Maotian Wang,Siyu Ji
Fan Du
Caspases are well recognized and studied as the central executioners of apoptosis, while recent studies have unraveled their diverse roles beyond cell apoptotic function.One emerging area of interest is non-apoptotic caspase activity as a p...
Eric Hawrylyshyn,Marcia Ferraz,Biankha Chan et al.
Eric Hawrylyshyn et al.
Somatic cell-based reproductive technologies (SCRTs) offer a promising approach for preserving the genetic diversity of threatened species. SCRTs require the nuclear reprogramming of a donor somatic cell, which has low success rates (1-5%) ...
Terpenes as Modulators of Nociceptive Signaling: Behavioral and Molecular Insights from Caenorhabditis elegans [0.03%]
来源于秀丽隐杆线虫的行为和分子研究揭示单萜通过调节痛觉信号传递止痛的新机制
Kaoutar Benkhraba,Fatma Boujenoui,Jesus David Castaño et al.
Kaoutar Benkhraba et al.
Terpenes such as Limonene (LIMO) and β-Caryophyllene (BC) have demonstrated pain-modulating properties, potentially through interactions with the TRPV1 receptor. This study examines the antinociceptive effects of four terpenes derived from...
Jessica Semmelrock,Hans-Joachim Wieden
Jessica Semmelrock
The non-canonical translation factor OBG-like ATPase 1 (OLA1) is conserved across all domains of life. OLA1 has been studied for decades by many groups, often under different names such GBP45, GTBP9, DOC45, PTD004, EngD, YyaF, Ybr025c, and ...
Acyl-protein thioesterase 1 (LYPLA1) activity promotes the growth of MDA-MB-468 triple-negative breast cancer cells [0.03%]
酰基蛋白质硫酯酶1(LYPLA1)活性可促进三阴性乳腺癌MDA-MB-468细胞的生长
Michael Salsaa,Mahtab Tavasoli,Haggag S Zein et al.
Michael Salsaa et al.
Protein S-acylation is a lipid-based, often reversible post-translational modification that can regulate many aspects of protein behavior, including subcellular localization, proteininteractions, and activity. Emerging evidence has identifi...
Characterization of IFN-γ mediated cellular responses in the HMC3 cell line [0.03%]
干扰γ诱导的小胶质细胞分化的HMC3细胞系中的炎症反应的表征
Esmé Sylvia Marina Franck,Jasmyne Angela Storm,Jueqin Lu et al.
Esmé Sylvia Marina Franck et al.
Microglia, the primary immune cells of the brain parenchyma, play critical roles in neurodevelopment and homeostasis. The human microglia clone 3 (HMC3) cell line has long been used to explore microglia dynamics. However, recent evidence su...
Structural Determinants for the Monomeric and Phospholipid-Transport P4B-ATPase via comprehensive in silico analysis of P4-ATPase structures [0.03%]
基于P4-ATP酶结构的全面计算分析确定单体和磷脂转运P4B-ATP酶的结构决定因素
Kadambari Vijay Sai,Danny Farhat,Sai Anvithaa Sundar Rajan et al.
Kadambari Vijay Sai et al.
The P4-ATPase family of phospholipid flippases plays a critical role in maintenance of membrane asymmetry and cellular protein traffic and eukaryotic homeostasis. Several structures of these phospholipid flippases have been resolved, along ...
Design and Biological Evaluation of Novel Biphenyl-Dihydropyrimidinone Derivatives with Selective Anticancer and Non-Genotoxic Profiles [0.03%]
一类新型苯并二氢嘧啶酮衍生物的设计、合成及其抗肿瘤活性与遗传毒性评价研究
Mehmet Fidan,Arif Ayar,Aliye Gediz Ertürk et al.
Mehmet Fidan et al.
This study reports the p-toluenesulfonic acid-catalyzed synthesis of three novel 3,4-dihydropyrimidin-2(1H)-one derivatives (4a = PMe, 4b = PPh, 4c = PNO₂) based on a 1,1'-biphenyl, and the evaluation of their cytotoxic, genotoxic, antioxi...
AXIN1-Dependent Regulation of Lung Macrophage MHC II Expression Shapes CD4⁺ T Cell Responses during BCG Infection [0.03%]
AXIN1依赖的肺巨噬细胞MHCII表达调节在BCG感染期间塑形CD4+T细胞反应
Xiaoling Liang,Ruoying Chen,Yuerong Zeng et al.
Xiaoling Liang et al.
Tuberculosis (TB), caused by Mycobacterium tuberculosis (M. tb), continues to be the second leading cause of infectious disease-related mortality globally, surpassed only by COVID-19. The Wnt/β-catenin signaling pathway is involved in regu...