Nicotine and cotinine enhance SARS-CoV-2 entry through distinct but complementary mechanisms in human respiratory epithelial cells [0.03%]
尼古丁和可替宁通过不同的但互补的机制促进SARS-CoV-2进入人类呼吸道上皮细胞
Jiheng Xu,Huei-Wei Chan,Rui Yang et al.
Jiheng Xu et al.
Previous epidemiological studies have shown that E-cigarette and tobacco users are more likely to develop COVID-19 symptoms than non-users. To investigate the underlying mechanisms, we examined the effects of nicotine, the major component o...
Molecular basis for protection and cross-protection by human antibodies targeting the parainfluenza virus hemagglutinin-neuraminidase protein [0.03%]
人针对副流感病毒血凝素-神经氨酸酶蛋白的抗体保护和交叉保护的分子基础
Katelyn D McCaffrey,Behrouz Ghazi Esfahani,Mohamed A Elbehairy et al.
Katelyn D McCaffrey et al.
Human parainfluenza viruses (PIVs) are a leading cause of respiratory illness, particularly in vulnerable populations, where infection can lead to severe disease. Despite their clinical impact, there are currently no licensed vaccines or ef...
Coronavirus Nsp15 endoribonuclease: linking viral RNA regulation to immune evasion and viral fitness [0.03%]
冠状病毒Nsp15内切核糖核酸酶：连接病毒RNA调节、免疫逃避和病毒适应性
Xueying Liang,Xiang Chi,Xufang Deng
Xueying Liang
Coronavirus nonstructural protein 15 (Nsp15) is a conserved uridine-preferring endoribonuclease (EndoU). Studies using mouse hepatitis virus (MHV), SARS-CoV-2, and other coronaviruses have shown that Nsp15 associates with replication-transc...
Guohao Wang,Chengyan Zhou,Qingqing Zhou et al.
Guohao Wang et al.
Koch's postulates remain unachievable for most crustacean viruses due to uncultivable pathogens and chronic infection dynamics. We propose viromic Koch's postulates, a culture-independent framework, to address this limitation. The modified ...
Enhanced replication and immunogenicity of a genome-modified non-replicating vaccinia virus Tiantan strain for robust low-dose protection in mice [0.03%]
改良后的天坛株减毒痘病毒免疫原性增强，在小鼠中可实现低剂量高效保护
Jiao Ren,Shiyuan Liu,Hang Yuan et al.
Jiao Ren et al.
A non-replicating Tiantan strain-based vaccinia virus (NTV) holds significant application prospects for vaccination and gene therapy and has recently entered clinical trials as a novel and safer vaccine candidate against monkeypox. However,...
ACE2-fused nanobody targeting a cryptic RBD epitope broadly neutralizes SARS-like viruses [0.03%]
具有靶向隐蔽RBD表位的ACE2融合纳米抗体可广谱中和SARS样病毒
Weihong Zeng,Guanying Zhang,Huan Ma et al.
Weihong Zeng et al.
The SARS-CoV-2 (SARS2) and SARS-CoV (SARS1) viruses pose significant threats due to their high mutation rates and potential for cross-species transmission, which has rendered many antibodies ineffective. To counter potential future emergenc...
Nanoparticles for antiviral nucleotide analogs: approaches and analytical challenges [0.03%]
用于抗病毒核苷类似物的纳米颗粒：方法和分析挑战
Scott M Clifford,Matthew J Edwards,Woravimol Krittaphol et al.
Scott M Clifford et al.
Nucleoside and nucleotide analogs are an impactful class of antivirals, yet their efficacy is often limited by inefficient intracellular accumulation and enzymatic activation. Many analogs are administered as nucleoside prodrugs, dependent ...
Recovery of proofreading-impaired SARS-CoV-2 reveals a mutator phenotype and an ExoN activity threshold for viability [0.03%]
具有校对功能受损的SARS-CoV-2的回复突变揭示了突变器表型和外切酶活性阈值可支持其存活
Li He,Yuan-Wei Norman Su,Fushun Zhang et al.
Li He et al.
Coronaviruses (CoVs) replicate unusually large RNA genomes that necessitate proofreading by the 3'-to-5' exoribonuclease (ExoN) formed by nonstructural proteins 14 (nsp14) and 10 (nsp10). Previous studies suggested that inactivation of the ...
Siglec-1-targeted nanobodies restrict HIV-1 transmission and infection of dendritic cells [0.03%]
靶向 Siglec-1 的纳米抗体可限制 HIV-1 传播和感染树突状细胞
Shirley Man,Alsya J Affandi,Hendrik J Brink et al.
Shirley Man et al.
Human immunodeficiency virus 1 (HIV-1) remains a global health burden affecting over 39 million people worldwide, with approximately one million new infections occurring each year. Sexual transmission remains the predominant route of HIV-1 ...
AAV2 bypasses direct endosomal escape by using AAVR to access the trans-Golgi network en route to the nucleus [0.03%]
AAV2通过使用AAVR在向细胞核移动的过程中访问反式高尔基体网络来避开直接逃逸至内体的方式
Marti Cabanes-Creus,Sophia H Y Liao,Marta Pardo-Piñón et al.
Marti Cabanes-Creus et al.
Vectors based on the adeno-associated virus are widely used as delivery methods in gene therapy applications, yet understanding of the mechanisms governing its intracellular trafficking remains incomplete. Traditional models suggest that AA...