Analysis of Multiplexed Flow Cytometric Assays and Toxicogenomic Signatures for Genotoxicity Prediction: A Model Performance and Case Study Approach [0.03%]
用于遗传毒性预测的多重流式细胞仪分析和毒基因组特征的模型性能及案例研究方法学评估
Tomás Lagunas Jr,Fjodor Melnikov,Gabby Cole et al.
Tomás Lagunas Jr et al.
Genotoxicity assays play a significant role in protecting clinical trial subjects from potential increased risk of genotoxic hazard and cancer during drug development. Traditional genetic toxicology assays typically provide binary outcomes ...
Xiaowen Sun,Stephen D Dertinger,Azeddine Elhajouji et al.
Xiaowen Sun et al.
The genotoxic potential of chemicals must be evaluated in regulatory safety assessment settings, including but not limited to, the development of new pharmaceuticals, industrial chemicals, food and cosmetic ingredients, and agrochemicals. I...
Background Mutation Frequencies in TK6 and L5178Y Cells: Implications for Error-Corrected Sequencing [0.03%]
TK6和L5178Y细胞中的背景突变频率：对错误校正测序的意义
Jaime A Miranda,Azra Dad,Xuewei Qu et al.
Jaime A Miranda et al.
Several error-corrected sequencing (ECS) methods can detect ultralow-frequency mutations and support mutagenicity assessments. While ECS can be applied to any DNA-containing sample, spontaneous mutations that accumulate in immortalized cell...
Transferability, Reproducibility and Sensitivity of Mutation Quantification by Duplex Sequencing [0.03%]
双联测序的突变定量的可移植性、可重复性和敏感度
Shaofei Zhang,Barbara L Parsons,Devon Fitzgerald et al.
Shaofei Zhang et al.
Duplex Sequencing (DS) is an ultra-accurate, error-corrected next generation sequencing (ecNGS) technology for mutation analysis. A working group (WG) within Health and Environmental Sciences Institute's Genetic Toxicology Technical Committ...
Identification of Hot and Cold Regions for Mutagenesis in the Escherichia coli tdk Gene: A Catalog of Mutational Sites [0.03%]
埃希氏大肠杆菌tdk基因突变热区和冷区的鉴定：突变位点目录
Katherine Douglas,Dana Sorensen,Arnav Saud et al.
Katherine Douglas et al.
We constructed a catalog of mutational sites in the tdk gene of Escherichia coli that consists of 378 different base pair substitutions at 245 different sites (base pairs). This allows us to examine the tendency of different sub-regions of ...
Assessment of the Genotoxic Hazard of Estuarine Sediments Using an Integrative Approach With LacZ Plasmid-Based Transgenic Mice [0.03%]
采用LacZ质粒转基因鼠综合评价河口底泥的遗传毒性危险性
Miguel Pinto,Joana Sacadura,Pedro M Costa et al.
Miguel Pinto et al.
Under the influence of multiple anthropogenic pressures, from industrial to agricultural activities, estuaries have long been regarded as particularly sensitive ecosystems to contamination. The present study aimed at investigating the genot...
Comparative Analysis of TA102 and WP2 uvrA(pKM101) Strains in Detecting Nitrosamine Mutagens in the Enhanced Ames Test [0.03%]
改良Ames试验中用TA102和WP2(uvrA(pkm101))菌株检测亚硝胺致突变物质的比较分析研究
Rushikesh M Shukla,Darshan T Valani,Chetan K Kajavadara et al.
Rushikesh M Shukla et al.
The Ames test is a fundamental assay for evaluating chemical mutagenicity, particularly for nitrosamines, which are widespread environmental and pharmaceutical contaminants. To improve sensitivity, regulatory agencies have endorsed the enha...
Ghazal Darfarin,Janice Pluth
Ghazal Darfarin
The mitochondria (mt) and nucleus engage in a dynamic bidirectional communication to maintain cellular homeostasis, regulating energy production, stress response, and cell fate. Anterograde signaling directs mt function, while retrograde si...
Application of Biological Modifiers to a Multiplexed, Human Cell-Based DNA Damage Assay Provides Mechanistic Information on Genotoxicity and Molecular Targets [0.03%]
生物调节物在人细胞DNA损伤检测中的应用可提供有关致突变性和分子靶标的机制信息
Steven M Bryce,Svetlana L Avlasevich,Adam Conrad et al.
Steven M Bryce et al.
This laboratory has reported that the combined use of In Vitro MicroFlow and MultiFlow assays provides information regarding chemicals' genotoxic mode of action (MoA). In an effort to go beyond MoA assessments, we incorporated a panel of bi...
Fertilized Avian Egg Fetal Liver Assays for Assessing DNA Damaging Potential of Chemicals: A Comparative Analysis With In Vitro and In Vivo Genotoxicity Assays and Rodent Carcinogenicity [0.03%]
用于评估化学品DNA损伤潜力的受精禽蛋胎儿肝测定：与体外和体内基因毒性试验及啮齿动物致癌性进行比较分析
Tetyana Kobets,Gary M Williams
Tetyana Kobets
The ability to produce direct DNA damage (genotoxicity), which underlies the carcinogenicity of various chemicals, is typically evaluated in a regulatory-approved battery of in vitro tests with potential in vivo follow-up. Growing concerns ...