Single-molecule live imaging of subunit interactions and exchange within cellular regulatory complexes [0.03%]
单分子活细胞影像技术观测细胞调控复合物中亚基的相互作用和交换现象
Thomas G W Graham,Claire Dugast-Darzacq,Gina M Dailey et al.
Thomas G W Graham et al.
Cells are built from vast networks of interdependent molecular interactions. Here, we combine proximity-assisted photoactivation (PAPA) with automated fast single-molecule tracking (fSMT) to probe subunit interactions within endogenous prot...
β-catenin functions as a molecular adapter for disordered cBAF interactions [0.03%]
β-catenin作为无序cBAF相互作用的分子适配器的功能
Yuen San Chan,Qinyu Gao,Sarah A Robinson et al.
Yuen San Chan et al.
BAF (SWI/SNF) chromatin remodelers engage binding partners to generate site-specific DNA accessibility. However, the basis for interaction between BAF and divergent binding partners has remained unclear. Here, we tested the hypothesis that ...
Structural mechanism of H3K27 demethylation and crosstalk with heterochromatin markers [0.03%]
H3K27去甲基化的结构机制及其与异染色质标记的相互作用
Chien-Chu Lin,Yani Zhao,Caroline A Foley et al.
Chien-Chu Lin et al.
Histone H3 lysine 27 trimethylation (H3K27me3) is a repressive histone modification that is a hallmark of facultative heterochromatin. H3K27me3 is installed by the polycomb repressive complex 2 (PRC2) and removed by KDM6 family Jumonji C (J...
The SWI/SNF-related protein SMARCA3 is a histone H3K23 ubiquitin ligase that regulates H3K9me3 in cancer [0.03%]
SWI/SNF家族成员SMARCA3是组蛋白H3K23泛素连接酶，调控肿瘤中的H3K9三甲基化
Ifé Akano,Jakob M Hebert,Rochelle L Tiedemann et al.
Ifé Akano et al.
Histone ubiquitination is a crucial post-translational modification (PTM) regulating chromatin function, yet many histone ubiquitination sites and the enzymes that control them remain poorly understood. Here, we identify SMARCA3, a SWI/SNF-...
Axel Delamarre,Benton Bailey,Jennifer Yavid et al.
Axel Delamarre et al.
Chromatin plays a pivotal role in genome expression, maintenance, and replication. To better understand chromatin organization, we developed a proximity-tagging method to map molecules that associate in 3D space. Using this method-PCP (prox...
Structural determinants of DANGEROUS MIX 3, an alpha/beta hydrolase that triggers NLR-mediated genetic incompatibility in plants [0.03%]
危险混杂3的结构决定因素，这是一种触发植物NLR介导的遗传不相容性的双组分α/β水解酶
Wei-Lin Wan,Gijeong Kim,Nayun Kim et al.
Wei-Lin Wan et al.
Genetic incompatibility occurs when a mismatched pair of immune components triggers autoimmunity in hybrid plants. Highly diversified NLR receptors are key culprits of this genetic conflict, recognizing host proteins from different origins ...
YTHDC1 lactylation regulates its phase separation to enhance target mRNA stability and promote RCC progression [0.03%]
YTHDC1 活化赖氨酸乳酸化调控其液-液相分离以增强靶mRNA稳定性及促进肾细胞癌进展
Chenyun Dai,Yuangui Tang,Huihui Yang et al.
Chenyun Dai et al.
The hypoxic and lactate-enriched microenvironment of renal cell carcinoma (RCC) provides favorable conditions for aberrant lysine lactylation (Kla). However, the functional role and mechanistic basis of Kla in RCC progression remain elusive...
Lactylation of XLF promotes non-homologous end-joining repair and chemoresistance in cancer [0.03%]
XLF 糖基化促进肿瘤 DNA 非同源末端连接修复及耐药性
Mingpeng Jin,Bingsong Huang,Xiaoning Yang et al.
Mingpeng Jin et al.
Metabolic reprogramming and DNA damage repair are essential in tumorigenesis and chemoresistance, yet their link remains elusive. Here, we show that LDHA deficiency impairs NHEJ and class switch recombination. Additionally, glycolysis-deriv...
Dale A Ramsden,Adam J Luthman
Dale A Ramsden
Ku is associated with non-homologous end joining (NHEJ), a double-strand break repair pathway, in all kingdoms of life, but Zhu et al.1 find that it gains an essential function in human cells down-regulating innate immune responses. ...
Feiyan Liu,Yu Zhou
Feiyan Liu
How m6A in different transcript regions regulates mRNA decay remains an intriguing question. Three studies from the labs of König,1 Jaffrey,2 and Steinmetz3 uncovered translation-dependent CDS-m6A decay: m6A in the coding sequence (CDS) in...