Requirements for establishment and epigenetic stability of mammalian heterochromatin [0.03%]
哺乳动物异染色质的建立与表观遗传稳定性的要求
Antonis Tatarakis,Harleen Saini,Juntao Yu et al.
Antonis Tatarakis et al.
Heterochromatic domains of DNA account for a large fraction of mammalian genomes and play critical roles in silencing transposons and genes, but the mechanisms that establish and maintain these domains are not fully understood. Here, we use...
Carson C Thoreen
Carson C Thoreen
In a recent publication in Cell, Wolin et al.1 describe a massively multiplexed variation of CLIP called SPIDR that allows the simultaneous profiling of targets of arbitrary numbers of RNA-binding proteins. Using SPIDR, they uncover unexpec...
Jenny Jin,Liam N Stiles,Brent R Stockwell
Jenny Jin
Damage to polyunsaturated fats causes ferroptotic cell death. In this issue, Deng et al.1 show that the incorporation of the fatty acid DHA into membranes is controlled by the protein UBXD8, as a natural mechanism to prevent overloading cel...
Jose Domingo Barrera-Paez,Carlos T Moraes
Jose Domingo Barrera-Paez
In this issue of Molecular Cell, Xiang et al.1 provided insights into the mechanism and structure-guided engineering of DdCBE for mitochondrial DNA base editing. More precise editing was achieved by better defining the editing window. ...
Biochemical reconstitution of sister chromatid cohesion establishment during DNA replication [0.03%]
DNA复制过程中姊妹染色单体粘连建立的生化重构
Masashi Minamino,John F X Diffley,Frank Uhlmann
Masashi Minamino
Concomitant with DNA replication, the ring-shaped cohesin complex encircles both newly synthesized sister chromatids, enabling their faithful segregation during cell divisions. Our molecular understanding of how cohesin co-entraps both repl...
Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome [0.03%]
快速耗尽UPF1揭示了NMD调节的人类转录组的动态变化
Volker Boehm,Damaris Wallmeroth,Paul O Wulf et al.
Volker Boehm et al.
The RNA helicase UPF1 shapes the transcriptome as the core factor of nonsense-mediated mRNA decay (NMD). The essential role of UPF1 in human cells has impeded efforts to delineate its directly regulated transcripts and molecular function. T...
RAD51 is chromatin enriched and targetable in BRCA1-deficient cells [0.03%]
BRCA1缺陷细胞中RAD51在染色质上富集并可以被靶向作用
Min Peng,Silviana Lee,Hitha Gopalan Nair et al.
Min Peng et al.
BRCA1 mutant cancers are homologous recombination (HR) deficient, and their sensitivity to anti-cancer therapies such as poly (ADP-ribose) polymerase (PARP) inhibitors (PARPi) has long been attributed to this defect. Accordingly, the HR mar...
α-Synuclein aggregates inhibit ESCRT-III through sequestration and collateral degradation [0.03%]
α-突触核蛋白聚集物通过吸附和旁侧降解抑制ESCRT-III功能
Cole S Sitron,Victoria A Trinkaus,Ana Galesic et al.
Cole S Sitron et al.
α-Synuclein aggregation is a hallmark of Parkinson's disease and related synucleinopathies. Extracellular α-synuclein fibrils enter naive cells via endocytosis, followed by transit into the cytoplasm to seed endogenous α-synuclein aggreg...
Structural insights into DdCBE in action enable high-precision mitochondrial DNA editing [0.03%]
DdCBE作用机制的结构见解可实现高精度线粒体DNA编辑
Jiangchao Xiang,Wenchao Xu,Jing Wu et al.
Jiangchao Xiang et al.
DddA-derived cytosine base editor (DdCBE) couples transcription activator-like effector (TALE) arrays and the double-stranded DNA (dsDNA)-specific cytidine deaminase DddA to target mitochondrial DNA (mtDNA) for editing. However, structures ...
Protection against ferroptosis through maintaining homeostasis of docosahexaenoate-containing phospholipids [0.03%]
通过维持含有二十二碳六烯酸的磷脂稳态来抵御铁死亡
Yaqin Deng,Goncalo Vale,Yonggang Liang et al.
Yaqin Deng et al.
Although polyunsaturated phospholipids are vital for cellular functions, their overaccumulation renders cells vulnerable to ferroptosis. It remains unclear how cells exposed to excess polyunsaturated fatty acids (PUFAs) prevent their over-i...