Size fractionated NET-Seq reveals a conserved architecture of transcription units around yeast genes [0.03%]
大小分级的NET-Seq揭示了酵母基因周围的转录单元的保守结构
Shidong Xi,Tania Nguyen,Struan Murray et al.
Shidong Xi et al.
Genomes from yeast to humans are subject to pervasive transcription. A single round of pervasive transcription is sufficient to alter local chromatin conformation, nucleosome dynamics and gene expression, but is hard to distinguish from bac...
Mariona Nadal-Ribelles,Carme Solé,Eulalia de Nadal et al.
Mariona Nadal-Ribelles et al.
The field of single-cell omics has transformed our understanding of biological processes and is constantly advancing both experimentally and computationally. One of the most significant developments is the ability to measure the transcripto...
Implication of polymerase recycling for nascent transcript quantification by live cell imaging [0.03%]
聚合酶循环对活细胞成像定量新生转录本的含义
Olivia Kindongo,Guillaume Lieb,Benjamin Skaggs et al.
Olivia Kindongo et al.
Transcription enables the production of RNA from a DNA template. Due to the highly dynamic nature of transcription, live-cell imaging methods play a crucial role in measuring the kinetics of this process. For instance, transcriptional burst...
MiniRead: A simple and inexpensive do-it-yourself device for multiple analyses of micro-organism growth kinetics [0.03%]
迷你阅读：一个简单且经济的自制设备，用于微生物生长动力学的多种分析
Matthieu Falque,Aurélie Bourgais,Fabrice Dumas et al.
Matthieu Falque et al.
Fitness in micro-organisms can be proxied by growth parameters on different media and/or temperatures. This is achieved by measuring optical density at 600 nm using a spectrophotometer, which measures the effect of absorbance and side scatt...
Identification of heparin-binding proteins expressed on Trichosporon asahii cell surface [0.03%]
糙皮侧孢表面表达的肝素结合蛋白的鉴定
Tomoe Ichikawa,Yuka Ikeda,Jumpei Sadanaga et al.
Tomoe Ichikawa et al.
Trichosporon asahii is a pathogenic yeast that cause trichosporonosis. T. asahii exhibits several colony morphologies, such as white (W)- or off-white (O)-type, which may affect virulence. In this study, we compared the expression pattern o...
An ultra high-throughput, massively multiplexable, single-cell RNA-seq platform in yeasts [0.03%]
一种用于酵母的超高效、高度多重化的单细胞RNA测序平台
Leandra Brettner,Rachel Eder,Kara Schmidlin et al.
Leandra Brettner et al.
Yeasts are naturally diverse, genetically tractable, and easy to grow such that researchers can investigate any number of genotypes, environments, or interactions thereof. However, studies of yeast transcriptomes have been limited by the pr...
Budding yeast as an ideal model for elucidating the role of N6-methyladenosine in regulating gene expression [0.03%]
芽状酵母作为阐明N6-甲基腺苷在调控基因表达中的作用的理想模型
Waleed S Albihlal,Wei Yee Chan,Folkert J van Werven
Waleed S Albihlal
N6-methyladenosine (m6A) is a highly abundant and evolutionarily conserved messenger RNA (mRNA) modification. This modification is installed on RRACH motifs on mRNAs by a hetero-multimeric holoenzyme known as m6A methyltransferase complex (...
Baptiste Piguet,Jonathan Houseley
Baptiste Piguet
Transcription presents challenges to genome stability both directly, by altering genome topology and exposing single-stranded DNA to chemical insults and nucleases, and indirectly by introducing obstacles to the DNA replication machinery. S...
Schizosaccharomyces versatilis represents a distinct evolutionary lineage of fission yeast [0.03%]
Schizosaccharomyces versatilis 代表了裂殖酵母的一个独特的进化谱系
Graham J Etherington,Elisa Gomez Gil,Wilfried Haerty et al.
Graham J Etherington et al.
The fission yeast species Schizosaccharomyces japonicus is currently divided into two varieties-S. japonicus var. japonicus and S. japonicus var. versatilis. Here we examine the var. versatilis isolate CBS5679. The CBS5679 genome shows 88% ...
Nakazawaea atacamensis f.a., sp. nov. a novel nonconventional fermentative ascomycetous yeast species from the Atacama Desert [0.03%]
纳卡萨韦属阿塔卡马氏新种非常规发酵子囊酵母菌种来自于阿塔卡马沙漠
Macarena Araya,Pablo Villarreal,Tomás Moyano et al.
Macarena Araya et al.
In this study, we describe Nakazawaea atacamensis f. a., sp. nov., a novel species obtained from Neltuma chilensis plant samples in Chile's hyperarid Atacama Desert. In total, three strains of N. atacamensis were obtained from independent N...