From Yeast to Therapeutics: Modeling Neurodegenerative Diseases in Saccharomyces cerevisiae [0.03%]
从酵母到治疗药物——在酿酒酵母中模拟神经退行性疾病
Jose Ribamar Ferreira-Junior,Vittoria de Lima Camandona,Mario H Barros
Jose Ribamar Ferreira-Junior
Here, we review the use of Saccharomyces cerevisiae as a powerful model organism for studying cellular processes implicated in neurodegenerative disorders, including stress responses, proteostasis impairment, and vesicle trafficking defects...
18S and 25S Exonuclease Resistant Ribosomal RNA Molecules Are Produced by 5'-End Modification During TOR Inhibition [0.03%]
靶向抑制TOR可导致rRNA 5'端修饰从而诱导产生抗核酸外切酶18S和25S rRNA分子
Miguel A Rocha,Gowda Bhavani,Jacob Fleischmann
Miguel A Rocha
Saccharomyces cerevisiae yeast cells have been shown to produce 18S and 25S ribosomal RNA molecules that are resistant to degradation by exonucleases, which require a 5' monophosphate for activity. These resistant RNA species accumulate dur...
Polyphosphate Accumulation Is Determined by Zinc and Inositol in Saccharomyces cerevisiae [0.03%]
锌和肌醇决定酿酒酵母多磷酸盐的积累
Alexander Deitert,Makarius Baier,Roy Eerlings et al.
Alexander Deitert et al.
Polyphosphate (polyP) is an intriguing polymer with diverse biological and industrial applications. Chemical polyP production is energy-intensive and limited in chain length at large-scale production. Alternatively, biological production of...
Characterization of Vector Elements and Soluble Expression of Pcv3 Cap Protein in Kluyveromyces Marxianus [0.03%]
Pcv3 Cap蛋白的克鲁维氏马克斯克雅Wei菌可溶性表达元件构建及鉴定
Jiamei Li,Xuechen Yang,Mingyue Xu et al.
Jiamei Li et al.
The yeast Kluyveromyces marxianus (K. marxianus), characterized by its thermotolerance and rapid growth, is emerging as a promising new platform organism for the production of recombinant proteins. In this study, we constructed an expressio...
Nadine Elpida Tatto,Lina Heistinger,Corinna Rebnegger et al.
Nadine Elpida Tatto et al.
The methylotrophic yeast Komagataella phaffii (formerly known as Pichia pastoris) is an essential host for biotechnological production. Here, we present the complete and annotated genome sequence of CBS 2612 T = NRRL Y-7556T, the type strai...
Effects of Backslopping on Yeast Diversity and the Volatile Profile of Tarhana [0.03%]
backslopping对Tarhana中酵母多样性和挥发性化合物的影响
Burcu Ozel,Bilal Agirman,Omer Simsek et al.
Burcu Ozel et al.
The primary challenge in tarhana production is the occurrence of spontaneous fermentation, which leads to non-standardized products. Thus, we investigated the effects of backslopping, a traditional method for inoculating fermented foods, on...
CRISPR/Cas9-Mediated Construction of a YPS Gene-Deficient Komagataella phaffii Strain for Enhanced Expression of BIAP Ⅱ [0.03%]
基于CRISPR-Cas9的酵母YPS基因敲除及毕赤氏酵母表达BIAP Ⅱ抗体增强效应研究
Haichao Li,Ping Gui,Xiao Li et al.
Haichao Li et al.
Multiple isoforms of bovine intestinal alkaline phosphatase (BIAP) have been identified, among which type Ⅱ (BIAP Ⅱ) exhibits the highest specific activity. While Komagataella phaffii has been successfully employed for the secretory expre...
Sucrose-Induced Transcriptomic Response in Ogataea polymorpha TBRC 4839 Reveals its Potential for Recombinant Protein Production [0.03%]
蔗糖响应的Ogataea polymorpha TBRC 4839基因表达谱揭示其在重组蛋白生产中的潜在应用
Somsak Likhitrattanapisal,Chitwadee Phithakrotchanakoon,Aekkachai Puseenam et al.
Somsak Likhitrattanapisal et al.
The thermotolerant yeast Ogataea polymorpha TBRC 4839 is a promising host for heterologous protein expression using sucrose and molasses as low-cost carbon sources, making it suitable for industrial applications. This study analyzed the gen...
Taxogenomic Analysis of a Novel Yeast Species, Lachancea rosae Sp. Nov. F.A., Isolated From the Wild Rose Rosa californica [0.03%]
从野生蔷薇玫瑰中分离到的一种新型酵母Lachancea rosae的税基因组分析
Yakendra Bajgain,Quinn K Langdon,Cara M Krien et al.
Yakendra Bajgain et al.
A novel Saccharomycotina yeast strain, yHQL494, was isolated from the rose hip of the wild rose Rosa californica from Castle Crags State Park, California, USA. Phylogenetic analyses of both whole genome data and the sequences from the D1/D2...
Exploiting the Geranylgeranyl-Pyrophosphate-Sensing N-Terminal Domain of HMG-CoA Reductase 2 to Regulate Farnesyl Pyrophosphate Synthase (Erg20p) for Improved Monoterpene Production in Saccharomyces cerevisiae [0.03%]
利用3-羟基-3-甲基戊二酸单酰辅酶A还原酶2的古洛糖醇-P焦磷酸盐感应N端结构域调节酿酒酵母中的法尼基焦磷酸合成酶（Erg20p）以提高单萜生产
Zeyu Lu,Samuel Evans,Liam McDonnell et al.
Zeyu Lu et al.
Dynamic downregulation of the endogenous farnesyl pyrophosphate (FPP) synthase (Erg20p) is crucial to engineer heterologous monoterpene production in the yeast Saccharomyces cerevisiae. FPP downstream metabolite geranylgeranyl pyrophosphate...